Despite extensive use in clinical practice, the mechanisms of propofol action on sodium channels are not fully understood. Yang et al. incorporate complementary biophysical approaches (electrophysiology and molecular dynamics simulations) to demonstrate that propofol inhibits two prokaryotic voltage-gated sodium channels, NaChBac and NavMs, by modulating both activation and inactivation gating.

The fast block to polyspermy is achieved in Xenopus laevis eggs by fertilization-induced depolarization. Wozniak et al. show that fertilization activates a signaling cascade involving phospholipase C, IP₃, and intracellular Ca²⁺ release, which induces depolarization via Ca²⁺-activated Cl⁻ efflux.

HCN channels generate rhythmic firing patterns in the brain and heart. Alvarez-Baron et al. identify key amino acids responsible for functional differences between cAMP-sensitive and insensitive HCN isoforms, revealing their role in communication between the nucleotide-binding domain and the pore.