Newest Articles
Spatiotemporal dynamics of calcium in dendritic spinesDendritic spines can have numerous different shapes, but how this affects the function of these neuronal subcompartments is unclear. Bell et al. develop a mathematical model that reveals how the size and shape of both the spine head and spine apparatus impact local calcium dynamics.
Ion-selective microelectrodes in voltage-clamped muscleMany cellular processes depend on the movement of ions across membranes, but current electrophysiological methods can only measure movements that generate an electrical current. Heiny et al. describe a new method to measure dynamic changes of intracellular ion concentration resulting from either electroneutral or electrogenic ion fluxes under voltage or current clamp.
The transport cycle of GlyT1 and GlyT2Erdem et al. compare the kinetics of the SLC6 family glycine transporters GlyT1 and GlyT2. Though the two transporters are rate-limited by distinct reaction steps, they both display high transport capacity, with the kinetics of GlyT1 sufficient to supply extracellular glycine to the NMDA receptor.
Myosin orientation in muscleHigh-resolution structural information is invaluable for understanding muscle function. Savich et al. use bifunctional spin labeling to determine the orientation of the myosin lever arm in muscle fibers at high resolution under ambient conditions, augmenting previous insights obtained from fluorescence and EM.
PI3K regulation of heart rate and IFHeart rate is set by the specialized tissue of the sinoatrial node. Lin et al. demonstrate a novel role for phosphoinositide 3-kinase in regulating cardiac pacemaking currents independently of the autonomic nervous system, a finding with relevance for diabetes, heart disease, and cancer.
Phosphoinositide dependence of TPC3Among the three two-pore channels (TPCs), TPC1 and TPC2 are selectively activated by PI(3,5)P2, while TPC3 has been considered not to respond to any PIP2s. Shimomura and Kubo find that TPC3 responds to both PI(3,5)P2 and PI(3,4)P2, but not to PI(4,5)P2.
Binding sites for CFTR potentiatorsIdentification of the binding sites for small molecules that alleviate gating defects in CFTR would assist rational drug design for the treatment of cystic fibrosis. Yeh et al. identify two potential binding sites for prototypical CFTR potentiators at the interface of CFTR’s two transmembrane domains.
Current Issue
July 1, 2019
Volume 151, No. 7
Submit your Research
Reviews & Opinions
- In Memoriam: David C. Gadsby, PhD
Andersen et al. commemorate the life of the eminent physiologist, David Gadsby.
- A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy
Taraska reviews the imaging methods that are being used to understand the structure of the plasma membrane at the molecular level.
Most Read
Advertisement
JGP study develops mathematical model that describes how calcium signaling could be influenced by spine geometry and ultrastructure.