The Journal of General Physiology
CrossRef
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 576K)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Colclasure, G. C.
Right arrow Articles by Parker, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Colclasure, G. C.
Right arrow Articles by Parker, J. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

The Journal of General Physiology, Vol 100, 1-10, Copyright © 1992 by The Rockefeller University Press

ARTICLES

Cytosolic protein concentration is the primary volume signal for swelling-induced [K-Cl] cotransport in dog red cells

GC Colclasure and JC Parker
Department of Medicine, University of North Carolina, Chapel Hill 27514.

Chloride-dependent K transport ([K-Cl] cotransport) in dog red cells is activated by cell swelling. Whether the volume signal is generated by a change in cell configuration or by the dilution of some cytosolic constituent is not known. To differentiate between these two alternatives we prepared resealed ghosts that, compared with intact red cells, had the same surface area and similar hemoglobin concentration, but a greatly diminished volume. Swelling-induced [K-Cl] cotransport was activated in the ghosts at a volume (20 fl) well below the activation volume for intact cells (70 fl), but at a similar hemoglobin concentration (30-35 g dry solids per 100 g wet weight). Ghosts made to contain 40% albumin and 60% hemoglobin showed activation of [K-Cl] cotransport at a concentration of cell solids similar to intact cells or ghosts containing only hemoglobin. [K-Cl] cotransport in the resealed ghosts became quiescent at a dry solid concentration close to that at which shrinkage-induced Na/H exchange became activated. These results support the notion that the primary volume sensor in dog red cells is cytosolic protein concentration. We speculate that macromolecular crowding is the mechanism by which cells initiate responses to volume perturbation.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
C. H. Joiner, R. Kirk Rettig, M. Jiang, M. Risinger, and R. S. Franco
Urea stimulation of KCl cotransport induces abnormal volume reduction in sickle reticulocytes
Blood, February 15, 2007; 109(4): 1728 - 1735.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Kang, J. Han, and D. Kim
Mechanism of inhibition of TREK-2 (K2P10.1) by the Gq-coupled M3 muscarinic receptor
Am J Physiol Cell Physiol, October 1, 2006; 291(4): C649 - C656.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. P. Minton
How can biochemical reactions within cells differ from those in test tubes?
J. Cell Sci., July 15, 2006; 119(14): 2863 - 2869.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. Kang, C. Choe, and D. Kim
Thermosensitivity of the two-pore domain K+ channels TREK-2 and TRAAK
J. Physiol., April 1, 2005; 564(1): 103 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hermoso, P. Olivero, R. Torres, A. Riveros, A. F. G. Quest, and A. Stutzin
Cell Volume Regulation in Response to Hypotonicity Is Impaired in HeLa Cells Expressing a Protein Kinase C {alpha} Mutant Lacking Kinase Activity
J. Biol. Chem., April 23, 2004; 279(17): 17681 - 17689.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
D. Kim and A. Pleumsamran
Cytoplasmic Unsaturated Free Fatty Acids Inhibit Atp-Dependent Gating of the G Protein-Gated K+ Channel
J. Gen. Physiol., March 1, 2000; 115(3): 287 - 304.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Guizouarn and R. Motais
Swelling activation of transport pathways in erythrocytes: effects of Cl-, ionic strength, and volume changes
Am J Physiol Cell Physiol, January 1, 1999; 276(1): C210 - C220.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. A. Hernandez and E. Cristina
Modeling cell volume regulation in nonexcitable cells: the roles of the Na+ pump and of cotransport systems
Am J Physiol Cell Physiol, October 1, 1998; 275(4): C1067 - C1080.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
F. LANG, G. L. BUSCH, M. RITTER, H. VOLKL, S. WALDEGGER, E. GULBINS, and D. HAUSSINGER
Functional Significance of Cell Volume Regulatory Mechanisms
Physiol Rev, January 1, 1998; 78(1): 247 - 306.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. S. Franco, H. Thompson, M. Palascak, and C. H. Joiner
The Formation of Transferrin Receptor-Positive Sickle Reticulocytes With Intermediate Density Is Not Determined by Fetal Hemoglobin Content
Blood, October 15, 1997; 90(8): 3195 - 3203.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Bang, Y. Kim, and D. Kim
TREK-2, a New Member of the Mechanosensitive Tandem-pore K+ Channel Family
J. Biol. Chem., June 2, 2000; 275(23): 17412 - 17419.
[Abstract] [Full Text] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents