|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 100, 89-114, Copyright © 1992 by The Rockefeller University Press
ARTICLES |
HF Clemo, JJ Feher and CM Baumgarten
Department of Physiology, Medical College of Virginia, Richmond 23298- 0551.
Previously we showed that atrial natriuretic factor (ANF) decreases cardiac cell volume by inhibiting ion uptake by Na+/K+/2Cl- cotransport. Digital video microscopy was used to study the role of guanosine 3',5'-monophosphate (cGMP) in this process in rabbit ventricular myocytes. Each cell served as its own control, and relative cell volumes (volume(test)/volume(control)) were determined. Exposure to 10 microM 8-bromo-cGMP (8-Br-cGMP) reversibly decreased cell volume to 0.892 +/- 0.007; the ED50 was 0.77 +/- 0.33 microM. Activating guanylate cyclase with 100 microM sodium nitroprusside also decreased cell volume to 0.889 +/- 0.009. In contrast, 8-bromo-adenosine 3',5'- monophosphate (8-Br-AMP; 0.01-100 microM) neither altered cell volume directly nor modified the response to 8-Br-cGMP. The idea that cGMP decreases cell volume by inhibiting Na+/K+/2Cl- cotransport was tested by blocking the cotransporter with 10 microM bumetanide (BUM) and removing the transported ions. After BUM treatment, 10 microM 8-Br-cGMP failed to decrease cell volume. Replacement of Na+ with N-methyl-D- glucamine or Cl- with methanesulfonate also prevented 8-Br-cGMP from shrinking cells. The data suggest that 8-Br-cGMP, like ANF, decreases ventricular cell volume by inhibiting Na+/K+/2Cl-cotransport. Evidence that ANF modulates cell volume via cGMP was also obtained. Pretreatment with 10 microM 8-Br-cGMP prevented the effect of 1 microM ANF on cell volume, and ANF suppressed 8-Br-cGMP-induced cell shrinkage. Inhibiting guanylate cyclase with the quinolinedione LY83583 (10 microM) diminished ANF-induced cell shrinkage, and inhibiting cGMP-specific phosphodiesterase with M&B22948 (Zaprinast; 100 microM) amplified the volume decrease caused by a low dose of ANF (0.01 microM) approximately fivefold. In contrast, neither 100 microM 8-Br-cAMP nor 50 microM forskolin affected the response to ANF. The effects of ANF, LY83583, and M&B29948 on cGMP levels in isolated ventricular myocytes were confirmed by 125I-cGMP radioimmunoassay. These data argue that ANF shrinks cardiac cells by increasing intracellular cGMP, thereby inhibiting Na+/K+/2Cl- cotransport. Basal cGMP levels also appear to modulate cell volume.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. L. del Rio, T. A. Dawson, B. D. Clymer, D. J. Paterson, and G. E. Billman Effects of acute vagal nerve stimulation on the early passive electrical changes induced by myocardial ischaemia in dogs: heart rate-mediated attenuation Exp Physiol, August 1, 2008; 93(8): 931 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. William, E. J. Hamilton, A. Garcia, H. Bundgaard, K. K. M. Chia, G. A. Figtree, and H. H. Rasmussen Natriuretic peptides stimulate the cardiac sodium pump via NPR-C-coupled NOS activation Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1067 - C1073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kogler, P. Schott, K. Toischer, H. Milting, P. N. Van, M. Kohlhaas, C. Grebe, A. Kassner, E. Domeier, N. Teucher, et al. Relevance of Brain Natriuretic Peptide in Preload-Dependent Regulation of Cardiac Sarcoplasmic Reticulum Ca2+ ATPase Expression Circulation, June 13, 2006; 113(23): 2724 - 2732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. O. Andersen, T. Skomedal, M. Enger, A. Fidjeland, T. Brattelid, F. O. Levy, and J.-B. Osnes {alpha}1-AR-mediated activation of NKCC in rat cardiomyocytes involves ERK-dependent phosphorylation of the cotransporter Am J Physiol Heart Circ Physiol, April 1, 2004; 286(4): H1354 - H1360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M Bers, W. H Barry, and S. Despa Intracellular Na+ regulation in cardiac myocytes Cardiovasc Res, March 15, 2003; 57(4): 897 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ramasamy, J. A. Payne, J. Whang, S. R. Bergmann, and S. Schaefer Protection of ischemic myocardium in diabetics by inhibition of electroneutral Na+-K+-2Cl{-} cotransporter Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H515 - H522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Imai, B. Jiang, and A. J Pappano Mechanism for muscarinic inhibition of ICa(L) is determined by the path for elevating cyclic AMP in cardiac myocytes Cardiovasc Res, August 1, 2001; 51(2): 331 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kishimoto, K. Rossi, and D. L. Garbers A genetic model provides evidence that the receptor for atrial natriuretic peptide (guanylyl cyclase-A) inhibits cardiac ventricular myocyte hypertrophy PNAS, February 8, 2001; (2001) 51625598. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. d. P. Gomez and E. Nasi Light Transduction in Invertebrate Hyperpolarizing Photoreceptors: Possible Involvement of a Go-Regulated Guanylate Cyclase J. Neurosci., July 15, 2000; 20(14): 5254 - 5263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. Lamb and T. J. Barna Endothelium modulates anion channel-dependent aortic contractions to iodide Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1527 - H1536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Hume, D. Duan, M. L. Collier, J. Yamazaki, and B. Horowitz Anion Transport in Heart Physiol Rev, January 1, 2000; 80(1): 31 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Clemo, B. S. Stambler, and C. M. Baumgarten Swelling-Activated Chloride Current Is Persistently Activated in Ventricular Myocytes From Dogs With Tachycardia-Induced Congestive Heart Failure Circ. Res., February 5, 1999; 84(2): 157 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. O. Andersen, M. Enger, G. H. Thoresen, T. Skomedal, and J.-B. Osnes alpha 1-Adrenergic activation of myocardial Na-K-2Cl cotransport involving mitogen-activated protein kinase Am J Physiol Heart Circ Physiol, August 1, 1998; 275(2): H641 - H652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Clemo, B. S. Stambler, and C. M. Baumgarten Persistent Activation of a Swelling-Activated Cation Current in Ventricular Myocytes From Dogs With Tachycardia-Induced Congestive Heart Failure Circ. Res., July 27, 1998; 83(2): 147 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. LANG, G. L. BUSCH, M. RITTER, H. VOLKL, S. WALDEGGER, E. GULBINS, and D. HAUSSINGER Functional Significance of Cell Volume Regulatory Mechanisms Physiol Rev, January 1, 1998; 78(1): 247 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Mihailidou, K. A. Buhagiar, and H. H. Rasmussen Na+ influx and Na+-K+ pump activation during short-term exposure of cardiac myocytes to aldosterone Am J Physiol Cell Physiol, January 1, 1998; 274(1): C175 - C181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Clemo and C. M. Baumgarten Swelling-activated Gd3+-sensitive Cation Current and Cell Volume Regulation in Rabbit Ventricular Myocytes J. Gen. Physiol., September 1, 1997; 110(3): 297 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Doyle, S. K. Ambler, J. Upshaw-Earley, A. Bastawrous, G. E. Goings, and E. Page Type B Atrial Natriuretic Peptide Receptor in Cardiac Myocyte Caveolae Circ. Res., July 19, 1997; 81(1): 86 - 91. [Abstract] [Full Text]
|
|
|
|
|
|
Z. Wang, T. Mitsuiye, S. A. Rees, and A. Noma Regulatory Volume Decrease of Cardiac Myocytes Induced by {beta}-Adrenergic Activation of the Cl- Channel in Guinea Pig J. Gen. Physiol., July 1, 1997; 110(1): 73 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Clemo and C. M. Baumgarten cGMP and Atrial Natriuretic Factor Regulate Cell Volume of Rabbit Atrial Myocytes Circ. Res., October 1, 1995; 77(4): 741 - 749. [Abstract] [Full Text]
|
|
|
|
|
|
I. Kishimoto, K. Rossi, and D. L. Garbers A genetic model provides evidence that the receptor for atrial natriuretic peptide (guanylyl cyclase-A) inhibits cardiac ventricular myocyte hypertrophy PNAS, February 27, 2001; 98(5): 2703 - 2706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-R. Li, M. Zhang, L. S. Satin, and C. M. Baumgarten Biphasic effects of cell volume on excitation-contraction coupling in rabbit ventricular myocytes Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1270 - H1277. [Abstract] [Full Text] [PDF]
|
|
|
|
