|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 103, 125-147, Copyright © 1994 by The Rockefeller University Press
ARTICLES |
J Garcia, T Tanabe and KG Beam
Department of Physiology, Colorado State University, Fort Collins 80523.
In both skeletal and cardiac muscle, the dihydropyridine (DHP) receptor is a critical element in excitation-contraction (e-c) coupling. However, the mechanism for calcium release is completely different in these muscles. In cardiac muscle the DHP receptor functions as a rapidly-activated calcium channel and the influx of calcium through this channel induces calcium release from the sarcoplasmic reticulum (SR). In contrast, in skeletal muscle the DHP receptor functions as a voltage sensor and as a slowly-activating calcium channel; in this case, the voltage sensor controls SR calcium release. It has been previously demonstrated that injection of dysgenic myotubes with cDNA (pCAC6) encoding the skeletal muscle DHP receptor restores the slow calcium current and skeletal type e-c coupling that does not require entry of external calcium (Tanabe, Beam, Powell, and Numa. 1988. Nature. 336:134-139). Furthermore, injection of cDNA (pCARD1) encoding the cardiac DHP receptor produces rapidly activating calcium current and cardiac type e-c coupling that does require calcium entry (Tanabe, Mikami, Numa, and Beam. 1990. Nature. 344:451-453). In this paper, we have studied the voltage dependence of, and the relationship between, charge movement, calcium transients, and calcium current in normal skeletal muscle cells in culture. In addition, we injected pCAC6 or pCARD1 into the nuclei of dysgenic myotubes and studied the relationship between the restored events and compared them with those of the normal cells. Charge movement and calcium currents were recorded with the whole cell patch-clamp technique. Calcium transients were measured with Fluo-3 introduced through the patch pipette. The kinetics and voltage dependence of the charge movement, calcium transients, and calcium current in dysgenic myotubes expressing pCAC6 were qualitatively similar to the ones elicited in normal myotubes: the calcium transient displayed a sigmoidal dependence on voltage and was still present after the addition of 0.5 mM Cd2+ + 0.1 mM La3+. In contrast, the calcium transient in dysgenic myotubes expressing pCARD1 followed the amplitude of the calcium current and thus showed a bell shaped dependence on voltage. In addition, the transient had a slower rate of rise than in pCAC6-injected myotubes and was abolished completely by the addition of Cd2+ + La3+.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. A. Bannister, S. Papadopoulos, C. S. Haarmann, and K. G. Beam Effects of inserting fluorescent proteins into the {alpha}1S II-III loop: insights into excitation-contraction coupling J. Gen. Physiol., June 29, 2009; 134(1): 35 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schredelseker, A. Dayal, T. Schwerte, C. Franzini-Armstrong, and M. Grabner Proper Restoration of Excitation-Contraction Coupling in the Dihydropyridine Receptor {beta}1-null Zebrafish Relaxed Is an Exclusive Function of the {beta}1a Subunit J. Biol. Chem., January 9, 2009; 284(2): 1242 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Bannister, M. Grabner, and K. G. Beam The {alpha}1S III-IV Loop Influences 1,4-Dihydropyridine Receptor Gating but Is Not Directly Involved in Excitation-Contraction Coupling Interactions with the Type 1 Ryanodine Receptor J. Biol. Chem., August 22, 2008; 283(34): 23217 - 23223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Garcia, T. Nabhani, and J. Garcia The calcium channel {alpha}2/{delta}1 subunit is involved in extracellular signalling J. Physiol., February 1, 2008; 586(3): 727 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Lorenzon and K. G. Beam Accessibility of Targeted DHPR Sites to Streptavidin and Functional Effects of Binding on EC Coupling J. Gen. Physiol., September 24, 2007; 130(4): 379 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Esposito, E. Germinario, M. Zanin, P. T. Palade, R. Betto, and D. Danieli-Betto Isoform switching in myofibrillar and excitation-contraction coupling proteins contributes to diminished contractile function in regenerating rat soleus muscle J Appl Physiol, April 1, 2007; 102(4): 1640 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Goonasekera, S. R. W. Chen, and R. T. Dirksen Reconstitution of local Ca2+ signaling between cardiac L-type Ca2+ channels and ryanodine receptors: insights into regulation by FKBP12.6 Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1476 - C1484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Szappanos, S. Smida-Rezgui, J. Cseri, C. Simut, J.-M. Sabatier, M. De Waard, L. Kovacs, L. Csernoch, and M. Ronjat Differential effects of maurocalcine on Ca2+ release events and depolarization-induced Ca2+ release in rat skeletal muscle J. Physiol., June 15, 2005; 565(3): 843 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Lorenzon, C. S. Haarmann, E. E. Norris, S. Papadopoulos, and K. G. Beam Metabolic Biotinylation as a Probe of Supramolecular Structure of the Triad Junction in Skeletal Muscle J. Biol. Chem., October 15, 2004; 279(42): 44057 - 44064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Payne, Z. Zheng, E. Gonzalez, Z.-M. Wang, M. L. Messi, and O. Delbono External Ca2+-dependent excitation-contraction coupling in a population of ageing mouse skeletal muscle fibres J. Physiol., October 1, 2004; 560(1): 137 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kugler, R. G. Weiss, B. E. Flucher, and M. Grabner Structural Requirements of the Dihydropyridine Receptor {alpha}1S II-III Loop for Skeletal-type Excitation-Contraction Coupling J. Biol. Chem., February 6, 2004; 279(6): 4721 - 4728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Alden and J. Garcia Dissociation of charge movement from calcium release and calcium current in skeletal myotubes by gabapentin Am J Physiol Cell Physiol, September 1, 2002; 283(3): C941 - C949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. El Oakley, O. C. Ooi, A. Bongso, and M. H. Yacoub Myocyte transplantation for myocardial repair: a few good cells can mend a broken heart Ann. Thorac. Surg., May 1, 2001; 71(5): 1724 - 1733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B Dietze, J Henke, H M Eichinger, F Lehmann-Horn, and W Melzer Malignant hyperthermia mutation Arg615Cys in the porcine ryanodine receptor alters voltage dependence of Ca2+ release J. Physiol., August 1, 2000; 526(3): 507 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Avila and R. T. Dirksen Functional Impact of the Ryanodine Receptor on the Skeletal Muscle L-Type Ca2+ Channel J. Gen. Physiol., April 1, 2000; 115(4): 467 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Dirksen and K. G. Beam Role of Calcium Permeation in Dihydropyridine Receptor Function: Insights into Channel Gating and Excitation-Contraction Coupling J. Gen. Physiol., September 1, 1999; 114(3): 393 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-M. Wang, M. Laura Messi, M. Renganathan, and O. Delbono Insulin-like growth factor-1 enhances rat skeletal muscle charge movement and L-type Ca2+ channel gene expression J. Physiol., April 15, 1999; 516(2): 331 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Harasztosi, I Sipos, L Kovacs, and W Melzer Kinetics of inactivation and restoration from inactivation of the L-type calcium current in human myotubes J. Physiol., April 1, 1999; 516(1): 129 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A W Trafford and D A Eisner Another trigger for the heartbeat J. Physiol., November 15, 1998; 513(1): 1 - 1. [Full Text] [PDF]
|
|
|
|
|
|
B Dietze, F Bertocchini, V Barone, A Struk, V Sorrentino, and W Melzer Voltage-controlled Ca2+ release in normal and ryanodine receptor type 3 (RyR3)-deficient mouse myotubes J. Physiol., November 15, 1998; 513(1): 3 - 9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nakai, T. Tanabe, T. Konno, B. Adams, and K. G. Beam Localization in the II-III Loop of the Dihydropyridine Receptor of a Sequence Critical for Excitation-Contraction Coupling J. Biol. Chem., September 25, 1998; 273(39): 24983 - 24986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Nakai, T. Ogura, F. Protasi, C. Franzini-Armstrong, P. D. Allen, and K. G. Beam Functional nonequality of the cardiac and skeletal ryanodine receptors PNAS, February 4, 1997; 94(3): 1019 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. El-Hayek, B. Antoniu, J. Wang, S. L. Hamilton, and N. Ikemoto Identification of Calcium Release-triggering and Blocking Regions of the II-III Loop of the Skeletal Muscle Dihydropyridine Receptor J. Biol. Chem., September 22, 1995; 270(38): 22116 - 22118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Oakley, N. J. Brand, P. B. Burton, M. C. McMullen, G. B. Adams, M. C. Poznansky, P. J. Barton, and M. H. Yacoub Efficiency of a high-titer retroviral vector for gene transfer into skeletal myoblasts J. Thorac. Cardiovasc. Surg., January 1, 1994; 115(1): 1 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Avila, K. M. S. O'Connell, L. A. Groom, and R. T. Dirksen Ca2+ Release through Ryanodine Receptors Regulates Skeletal Muscle L-type Ca2+ Channel Expression J. Biol. Chem., May 18, 2001; 276(21): 17732 - 17738. [Abstract] [Full Text] [PDF]
|
|
|
|
