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The Journal of General Physiology, Vol 103, 249-278, Copyright © 1994 by The Rockefeller University Press
ARTICLES |
T Hoshi, WN Zagotta and RW Aldrich
Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, California 94305.
Kinetics of single voltage-dependent Shaker potassium channels expressed in Xenopus oocytes were studied in the absence of fast N-type inactivation. Comparison of the single-channel first latency distribution and the time course of the ensemble average current showed that the activation time course and its voltage dependence are largely determined by the transitions before first opening. The open dwell time data are consistent with a single kinetically distinguishable open state. Once the channel opens, it can enter at least two closed states which are not traversed frequently during the activation process. The rate constants for the transitions among these closed states and the open state are nearly voltage-independent at depolarized voltages (> - 30 mV). During the deactivation process at more negative voltages, the channel can close directly to a closed state in the activation pathway in a voltage-dependent fashion.
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|
|
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|
|
|
|
|
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|
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|
|
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J. Zheng and F. J. Sigworth Selectivity Changes during Activation of Mutant Shaker Potassium Channels J. Gen. Physiol., August 1, 1997; 110(2): 101 - 117. [Abstract] [Full Text] [PDF]
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C.-C. Shieh, K. G. Klemic, and G. E. Kirsch Role of Transmembrane Segment S5 on Gating of Voltage-dependent K+ Channels J. Gen. Physiol., June 1, 1997; 109(6): 767 - 778. [Abstract] [Full Text] [PDF]
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S. Kellenberger, J. W. West, T. Scheuer, and W. A. Catterall Molecular Analysis of the Putative Inactivation Particle in the Inactivation Gate of Brain Type IIA Na+ Channels J. Gen. Physiol., May 1, 1997; 109(5): 589 - 605. [Abstract] [Full Text] [PDF]
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C. Mathes, J. J.C. Rosenthal, C. M. Armstrong, and W. F. Gilly Fast Inactivation of Delayed Rectifier K Conductance in Squid Giant Axon and Its Cell Bodies J. Gen. Physiol., April 1, 1997; 109(4): 435 - 448. [Abstract] [Full Text] [PDF]
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V. Avdonin, E. F. Shibata, and T. Hoshi Dihydropyridine Action on Voltage-dependent Potassium Channels Expressed in Xenopus Oocytes J. Gen. Physiol., February 1, 1997; 109(2): 169 - 180. [Abstract] [Full Text] [PDF]
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