|
|
|
|
|
|
|
||
Article |
The effects of sulfhydryl reduction/oxidation on the gating of large-conductance, Ca2+-activated K+ (maxi-K) channels were examined in excised patches from tracheal myocytes. Channel activity was modified by sulfhydryl redox agents applied to the cytosolic surface, but not the extracellular surface, of membrane patches. Sulfhydryl reducing agents dithiothreitol, β-mercaptoethanol, and GSH augmented, whereas sulfhydryl oxidizing agents diamide, thimerosal, and 2,2'-dithiodipyridine inhibited, channel activity in a concentration-dependent manner. Channel stimulation by reduction and inhibition by oxidation persisted following washout of the compounds, but the effects of reduction were reversed by subsequent oxidation, and vice versa. The thiol-specific reagents N-ethylmaleimide and (2-aminoethyl)methanethiosulfonate inhibited channel activity and prevented the effect of subsequent sulfhydryl oxidation. Measurements of macroscopic currents in inside-out patches indicate that reduction only shifted the voltage/nPo relationship without an effect on the maximum conductance of the patch, suggesting that the increase in nPo following reduction did not result from recruitment of more functional channels but rather from changes of channel gating. We conclude that redox modulation of cysteine thiol groups, which probably involves thiol/disulfide exchange, alters maxi-K channel gating, and that this modulation likely affects channel activity under physiological conditions.
Key Words: KCa channels • sulfhydryl • disulfide • thiol
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Hou, S. H. Heinemann, and T. Hoshi Modulation of BKCa Channel Gating by Endogenous Signaling Molecules Physiology, February 1, 2009; 24(1): 26 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Rogers, W. M. Chilian, I. N. Bratz, R. M. Bryan Jr., and G. M. Dick H2O2 activates redox- and 4-aminopyridine-sensitive Kv channels in coronary vascular smooth muscle Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1404 - H1411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Stowe, M. Aldakkak, A. K. S. Camara, M. L. Riess, A. Heinen, S. G. Varadarajan, and M.-T. Jiang Cardiac mitochondrial preconditioning by Big Ca2+-sensitive K+ channel opening requires superoxide radical generation Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H434 - H440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Zagorac, K. Yamaura, C. Zhang, R. J. Roman, and D. R. Harder The Effect of Superoxide Anion on Autoregulation of Cerebral Blood Flow Stroke, December 1, 2005; 36(12): 2589 - 2594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Xi, S. Y. Cheranov, and J. H. Jaggar Mitochondria-Derived Reactive Oxygen Species Dilate Cerebral Arteries by Activating Ca2+ Sparks Circ. Res., August 19, 2005; 97(4): 354 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Gutterman, H. Miura, and Y. Liu Redox Modulation of Vascular Tone: Focus of Potassium Channel Mechanisms of Dilation Arterioscler Thromb Vasc Biol, April 1, 2005; 25(4): 671 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Simon, D. Varela, A. L. Eguiguren, L. F. Diaz, F. Sala, and A. Stutzin Hydroxyl radical activation of a Ca2+-sensitive nonselective cation channel involved in epithelial cell necrosis Am J Physiol Cell Physiol, October 1, 2004; 287(4): C963 - C970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. C. Santarelli, J. Chen, S. H. Heinemann, and T. Hoshi The {beta}1 Subunit Enhances Oxidative Regulation of Large-Conductance Calcium-activated K+ Channels J. Gen. Physiol., September 27, 2004; 124(4): 357 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Miller, C. D. Tulbert, and D. W. Busija Rosuvastatin treatment reverses impaired coronary artery vasodilation in fructose-fed, insulin-resistant rats Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R157 - R160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Prasad and R. K. Goyal Differential modulation of voltage-dependent K+ currents in colonic smooth muscle by oxidants Am J Physiol Cell Physiol, March 1, 2004; 286(3): C671 - C682. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Kohler, A. Hills, and M. R. Blatt Control of Guard Cell Ion Channels by Hydrogen Peroxide and Abscisic Acid Indicates Their Action through Alternate Signaling Pathways Plant Physiology, February 1, 2003; 131(2): 385 - 388. [Full Text] [PDF]
|
|
|
|
 |
|
 D. Leszkiewicz and E. Aizenman A role for the redox site in the modulation of the NMDA receptor by light J. Physiol., December 1, 2002; 545(2): 435 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kloek, I. van Ark, N. Bloksma, F. De Clerck, F. P. Nijkamp, and G. Folkerts Glutathione and other low-molecular-weight thiols relax guinea pig trachea ex vivo: interactions with nitric oxide? Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L403 - L408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Erxleben, A. L. Everhart, C. Romeo, H. Florance, M. B. Bauer, D. A. Alcorta, S. Rossie, M. J. Shipston, and D. L. Armstrong Interacting Effects of N-terminal Variation and Strex Exon Splicing on slo Potassium Channel Regulation by Calcium, Phosphorylation, and Oxidation J. Biol. Chem., July 19, 2002; 277(30): 27045 - 27052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E Armstrong and W. M Roberts Rapidly inactivating and non-inactivating calcium-activated potassium currents in frog saccular hair cells J. Physiol., October 1, 2001; 536(1): 49 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-H. Zeng, J.-P. Ding, X.-M. Xia, and C. J. Lingle Gating Properties Conferred on Bk Channels by the {beta}3b Auxiliary Subunit in the Absence of Its Nh2- and Cooh Termini J. Gen. Physiol., June 1, 2001; 117(6): 607 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. D. Tang, H. Daggett, M. Hanner, M. L. Garcia, O. B. McManus, N. Brot, H. Weissbach, S. H. Heinemann, and T. Hoshi Oxidative Regulation of Large Conductance Calcium-Activated Potassium Channels J. Gen. Physiol., March 1, 2001; 117(3): 253 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hoshi and S. H Heinemann Regulation of cell function by methionine oxidation and reduction J. Physiol., February 15, 2001; 531(1): 1 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. Walters, M. Covarrubias, and J. S. Ellingson Potent inhibition of the aortic smooth muscle maxi-K channel by clinical doses of ethanol Am J Physiol Cell Physiol, October 1, 2000; 279(4): C1107 - C1115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R J Lang, J R Harvey, G J McPhee, and M F Klemm Nitric oxide and thiol reagent modulation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeci J. Physiol., June 1, 2000; 525(2): 363 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Brzezinska, D. Gebremedhin, W. M. Chilian, B. Kalyanaraman, and S. J. Elliott Peroxynitrite reversibly inhibits Ca2+-activated K+ channels in rat cerebral artery smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H1883 - H1890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. da Silva-Santos and J. Assreuy Long-Lasting Changes of Rat Blood Pressure to Vasoconstrictors and Vasodilators Induced by Nitric Oxide Donor Infusion: Involvement of Potassium Channels J. Pharmacol. Exp. Ther., July 1, 1999; 290(1): 380 - 387. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Abderrahmane, D. Salvail, M. Dumoulin, J. Garon, A. Cadieux, and E. Rousseau Direct Activation of KCa Channel in Airway Smooth Muscle by Nitric Oxide: Involvement of a Nitrothiosylation Mechanism? Am. J. Respir. Cell Mol. Biol., September 1, 1998; 19(3): 485 - 497. [Abstract] [Full Text]
|
|
|
|
|
|
J. Church, K. A Baxter, and J. G McLarnon pH modulation of Ca2+ responses and a Ca2+-dependent K+ channel in cultured rat hippocampal neurones J. Physiol., August 15, 1998; 511(1): 119 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Soto, C. Gonzalez, E. Lissi, C. Vergara, and R. Latorre Ca2+-activated K+ channel inhibition by reactive oxygen species Am J Physiol Cell Physiol, March 1, 2002; 282(3): C461 - C471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, K. Terata, N. J. Rusch, and D. D. Gutterman High Glucose Impairs Voltage-Gated K+ Channel Current in Rat Small Coronary Arteries Circ. Res., July 20, 2001; 89(2): 146 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Riesco-Fagundo, M. T. Perez-Garcia, C. Gonzalez, and J. R. Lopez-Lopez O2 Modulates Large-Conductance Ca2+-Dependent K+ Channels of Rat Chemoreceptor Cells by a Membrane-Restricted and CO-Sensitive Mechanism Circ. Res., August 31, 2001; 89(5): 430 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
