The Journal of General Physiology
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 246K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sudlow, L. C.
Right arrow Articles by Gillette, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sudlow, L. C.
Right arrow Articles by Gillette, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1295/1997//243/ $5.00
Journal of General Physiology, Volume 110, Number 3, 1997

Article

Cyclic AMP Levels, Adenylyl Cyclase Activity, and Their Stimulation by Serotonin Quantif ied in Intact Neurons

Leland C. Sudlow and Rhanor Gillette

From the Department of Molecular and Integrative Physiology and the Neuroscience Program, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801

In molluscan central neurons that express cAMP-gated Na+ current (INa,cAMP), estimates of the cAMP binding affinity of the channels have suggested that effective native intracellular cAMP concentrations should be much higher than characteristic of most cells. Using neurons of the marine opisthobranch snail Pleurobranchaea californica, we applied theory and conventional voltage clamp techniques to use INa,cAMP to report basal levels of endogenous cAMP and adenylyl cyclase, and their stimulation by serotonin. Measurements were calibrated to iontophoretic cAMP injection currents to enable expression of the data in molar terms. In 30 neurons, serotonin stimulated on average a 23-fold increase in submembrane [cAMP], effected largely by an 18-fold increase in adenylyl cyclase activity. Serotonin stimulation of adenylyl cyclase and [cAMP] was inversely proportional to cells' resting adenylyl cyclase activity. Average cAMP concentration at the membrane rose from 3.6 to 27.6 µM, levels consistent with the expected cAMP dissociation constants of the INa,cAMP channels. These measures confirm the functional character of INa,cAMP in the context of high levels of native cAMP. Methods similar to those employed here might be used to establish critical characters of cyclic nucleotide metabolism in the many cells of invertebrates and vertebrates that are being found to express ion currents gated by direct binding of cyclic nucleotides.

Key Words: Pleurobranchaea • cyclic nucleotide–gated cation current • protein kinase A • H current

Address correspondence to Dr. L.C. Sudlow, Department of Molecular and Integrative Physiology, University of Illinois, Urbana-Champaign, 524 Burrill Hall, 407 S. Goodwin Ave., Urbana, IL 61801. Fax: 217-333-1133; E-mail rhanor{at}uiuc.edu. Address reprint requests to Dr. R. Gillette, Department of Molecular and Integrative Physiology, University of Illinois, Urbana-Champaign, 524 Burrill Hall, 407 S. Goodwin Ave., Urbana, IL 61801.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
V. A. Straub, J. Grant, M. O'Shea, and P. R. Benjamin
Modulation of Serotonergic Neurotransmission by Nitric Oxide
J Neurophysiol, February 1, 2007; 97(2): 1088 - 1099.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
N. G. Hatcher, L. C. Sudlow, L. L. Moroz, and R. Gillette
Nitric Oxide Potentiates cAMP-Gated Cation Current in Feeding Neurons of Pleurobranchaea californica Independent of cAMP and cGMP Signaling Pathways
J Neurophysiol, May 1, 2006; 95(5): 3219 - 3227.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
D. G. Bichet
Lithium, Cyclic AMP Signaling, A-Kinase Anchoring Proteins, and Aquaporin-2
J. Am. Soc. Nephrol., April 1, 2006; 17(4): 920 - 922.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. H. Schwartz
The many dimensions of cAMP signaling
PNAS, November 20, 2001; 98(24): 13482 - 13484.
[Full Text] [PDF]

Home page
 JGPHome page
T. C. Rich, K. A. Fagan, H. Nakata, J. Schaack, D. M.F. Cooper, and J. W. Karpen
Cyclic Nucleotide-Gated Channels Colocalize with Adenylyl Cyclase in Regions of Restricted Camp Diffusion
J. Gen. Physiol., August 1, 2000; 116(2): 147 - 162.
[Abstract] [Full Text] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents