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J. Gen. Physiol.,
Volume 111, Number 6, June 1, 1998 741-749
From the Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254
SliK, a K+ channel encoded by the Streptomyces KcsA gene, was expressed, purified, and reconstituted
in liposomes. A concentrative 86Rb+ flux assay was used to assess the ion transport properties of SliK. SliK-mediated ionic flux shows strong selectivity for K+ over Na+ and is inhibited by micromolar concentrations of Ba2+,
mirroring the basic permeation characteristic of eukaryotic K+ channels studied by electrophysiological methods.
86Rb+ uptake kinetics and equilibrium measurements also demonstrate that the purified protein is fully active.
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