|
|
|
|
|
|
|
||
Article |
To study the mechanism by which Ca2+, which enters during the odor response, is extruded during response recovery, recordings were made from isolated frog olfactory receptor cells using the suction pipette technique, while superfusing the olfactory cilia with solutions of modified ionic composition. When external Na+ was substituted with another cation, the response to odor was greatly prolonged. This prolongation of the response was similar irrespective of whether Na+ was replaced with Li+, which permeates the cyclic nucleotide-gated conductance, or choline, which does not. The prolonged current was greatly reduced by exposure to 300 µM niflumic acid, a blocker of the calcium-activated chloride channel, indicating that it is carried by this conductance, and abolished if Ca2+ was omitted from the external solution, demonstrating that Ca2+ influx is required for its generation. When the cilia were exposed to Na+-free solution after odor stimulation, the recovery of the response to a second stimulus from the adaptation induced by the first was greatly reduced. We conclude that a Na+-dependent Ca2+ extrusion mechanism is present in frog olfactory cilia and that it serves as the main mechanism that returns cytoplasmic Ca2+ concentration to basal levels after stimulation and mediates the normally rapid recovery of the odor response and the restoration of sensitivity after adaptation.
Key Words: olfactory receptor • calcium • adaptation
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Dando Examining the neglected side of calcium regulation in taste cells J. Physiol., December 1, 2009; 587(23): 5523 - 5524. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Waldeck, K. Vocke, N. Ungerer, S. Frings, and F. Mohrlen Activation and desensitization of the olfactory cAMP-gated transduction channel: identification of functional modules J. Gen. Physiol., November 16, 2009; 134(5): 397 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Saidu, S.D. Weeraratne, M. Valentine, R. Delay, and J. L. Van Houten Role of Plasma Membrane Calcium ATPases in Calcium Clearance from Olfactory Sensory Neurons Chem Senses, May 1, 2009; 34(4): 349 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pezier, Y. V. Bobkov, and B. W. Ache The Na+/Ca2+ Exchanger Inhibitor, KB-R7943, Blocks a Nonselective Cation Channel Implicated in Chemosensory Transduction J Neurophysiol, March 1, 2009; 101(3): 1151 - 1159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Kleene The Electrochemical Basis of Odor Transduction in Vertebrate Olfactory Cilia Chem Senses, November 1, 2008; 33(9): 839 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reisert, K.-W. Yau, and F. L. Margolis Olfactory marker protein modulates the cAMP kinetics of the odour-induced response in cilia of mouse olfactory receptor neurons J. Physiol., December 15, 2007; 585(3): 731 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Antolin and H. R. Matthews The effect of external sodium concentration on sodium-calcium exchange in frog olfactory receptor cells J. Physiol., June 1, 2007; 581(2): 495 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pezier, A. Acquistapace, M. Renou, J.-P. Rospars, and P. Lucas Ca2+ Stabilizes the Membrane Potential of Moth Olfactory Receptor Neurons at Rest and Is Essential for Their Fast Repolarization Chem Senses, May 1, 2007; 32(4): 305 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Weiler, S. Deutsch, and R. Apfelbach Combined Behavioral and c-Fos Studies Elucidate the Vital Role of Sodium for Odor Detection Chem Senses, September 1, 2006; 31(7): 641 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Boccaccio, L. Lagostena, V. Hagen, and A. Menini Fast Adaptation in Mouse Olfactory Sensory Neurons Does Not Require the Activity of Phosphodiesterase J. Gen. Physiol., July 31, 2006; 128(2): 171 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kaneko, F. Mohrlen, and S. Frings Calmodulin Contributes to Gating Control in Olfactory Calcium-activated Chloride Channels J. Gen. Physiol., May 30, 2006; 127(6): 737 - 748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Dougherty, G. A. Wright, and A. C. Yew Computational model of the cAMP-mediated sensory response and calcium-dependent adaptation in vertebrate olfactory receptor neurons PNAS, July 26, 2005; 102(30): 10415 - 10420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bhandawat, J. Reisert, and K.-W. Yau Elementary Response of Olfactory Receptor Neurons to Odorants Science, June 24, 2005; 308(5730): 1931 - 1934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Baryshnikov, O. A. Rogachevskaja, and S. S. Kolesnikov Calcium Signaling Mediated by P2Y Receptors in Mouse Taste Cells J Neurophysiol, November 1, 2003; 90(5): 3283 - 3294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Delgado, M. V. Saavedra, O. Schmachtenberg, J. Sierralta, and J. Bacigalupo Presence of Ca2+-Dependent K+ Channels in Chemosensory Cilia Support a Role in Odor Transduction J Neurophysiol, September 1, 2003; 90(3): 2022 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reisert, P. J. Bauer, K.-W. Yau, and S. Frings The Ca-activated Cl Channel and its Control in Rat Olfactory Receptor Neurons J. Gen. Physiol., August 25, 2003; 122(3): 349 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. B. Kaupp and R. Seifert Cyclic Nucleotide-Gated Ion Channels Physiol Rev, July 1, 2002; 82(3): 769 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takeuchi and T. Kurahashi Photolysis of caged cyclic AMP in the ciliary cytoplasm of the newt olfactory receptor cell J. Physiol., June 15, 2002; 541(3): 825 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Cinelli, D. Wang, P. Chen, W. Liu, and M. Halpern Calcium Transients in the Garter Snake Vomeronasal Organ J Neurophysiol, March 1, 2002; 87(3): 1449 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reisert and H. R Matthews Simultaneous recording of receptor current and intraciliary Ca2+ concentration in salamander olfactory receptor cells J. Physiol., September 15, 2001; 535(3): 637 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reisert and H. R Matthews Responses to prolonged odour stimulation in frog olfactory receptor cells J. Physiol., July 1, 2001; 534(1): 179 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kaneko, T. Nakamura, and B. Lindemann Noninvasive measurement of chloride concentration in rat olfactory receptor cells with use of a fluorescent dye Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1387 - C1393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reisert and H. R Matthews Response properties of isolated mouse olfactory receptor cells J. Physiol., January 1, 2001; 530(1): 113 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zufall and T. Leinders-Zufall The Cellular and Molecular Basis of Odor Adaptation Chem Senses, August 1, 2000; 25(4): 473 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Danaceau and M. T. Lucero Electrogenic Na+/Ca2+ Exchange: A Novel Amplification Step in Squid Olfactory Transduction J. Gen. Physiol., June 1, 2000; 115(6): 759 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zufall, T. Leinders-Zufall, and C. A. Greer Amplification of Odor-Induced Ca2+ Transients by Store-Operated Ca2+ Release and Its Role in Olfactory Signal Transduction J Neurophysiol, January 1, 2000; 83(1): 501 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reisert and H R Matthews Adaptation of the odour-induced response in frog olfactory receptor cells J. Physiol., September 15, 1999; 519(3): 801 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Blaustein and W. J. Lederer Sodium/Calcium Exchange: Its Physiological Implications Physiol Rev, July 1, 1999; 79(3): 763 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. E. Dionne New Kid on the Block: A Role for the Na/Ca Exchanger in Odor Transduction J. Gen. Physiol., November 1, 1998; 112(5): 527 - 528. [Full Text] [PDF]
|
|
|
|
 |
|
 T. Leinders-Zufall, M. Ma, and F. Zufall Impaired Odor Adaptation in Olfactory Receptor Neurons after Inhibition of Ca2+/Calmodulin Kinase II J. Neurosci., July 15, 1999; 19(14): RC19 - RC19. [Abstract] [Full Text] [PDF]
|
|
|
|
