The Journal of General Physiology
Scientifica: Experts in Electrophysiology
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article
Right arrow Full Text
Right arrow Full Text (PDF, 403K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Narita, K.
Right arrow Articles by Kuba, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Narita, K.
Right arrow Articles by Kuba, K.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J. Gen. Physiol., Volume 112, Number 5, November 1, 1998 593-609

A Ca2+-induced Ca2+ Release Mechanism Involved in Asynchronous Exocytosis at Frog Motor Nerve Terminals

K. Narita,§ T. Akita,* M. Osanai,parallel T. Shirasaki,Dagger H. Kijima,** and K. Kuba*Dagger

From the * Department of Physiology, School of Medicine, Nagoya University, Showa-ku, Nagoya 466-8550, Japan; Dagger  Department of Physiology, Saga Medical School, Saga 849-8501, Japan; § Department of Physiology, Kawasaki Medical School, Kurashiki 701-0192, Japan; parallel  Department of Pharmacology, Tokyo Medical and Dental University, School of Medicine, Tokyo 113-8519, Japan;  Department of Physiology, Kansai Medical University, Moriguchi 570-0074, Japan; and ** Department of Physics, School of Science, Nagoya University, Nagoya 464-8602, Japan

The extent to which Ca2+-induced Ca2+ release (CICR) affects transmitter release is unknown. Continuous nerve stimulation (20-50 Hz) caused slow transient increases in miniature end-plate potential (MEPP) frequency (MEPP-hump) and intracellular free Ca2+ ([Ca2+]i) in presynaptic terminals (Ca2+-hump) in frog skeletal muscles over a period of minutes in a low Ca2+, high Mg2+ solution. Mn2+ quenched Indo-1 and Fura-2 fluorescence, thus indicating that stimulation was accompanied by opening of voltage-dependent Ca2+ channels. MEPP-hump depended on extracellular Ca2+ (0.05-0.2 mM) and stimulation frequency. Both the Ca2+- and MEPP-humps were blocked by 8-(N,N-diethylamino)octyl3,4,5-trimethoxybenzoate hydrochloride (TMB-8), ryanodine, and thapsigargin, but enhanced by CN-. Thus, Ca2+-hump is generated by the activation of CICR via ryanodine receptors by Ca2+ entry, producing MEPP-hump. A short interruption of tetanus (<1 min) during MEPP-hump quickly reduced MEPP frequency to a level attained under the effect of TMB-8 or thapsigargin, while resuming tetanus swiftly raised MEPP frequency to the previous or higher level. Thus, the steady/equilibrium condition balancing CICR and Ca2+ clearance occurs in nerve terminals with slow changes toward a greater activation of CICR (priming) during the rising phase of MEPP-hump and toward a smaller activation during the decay phase. A short pause applied after the end of MEPP- or Ca2+-hump affected little MEPP frequency or [Ca2+]i, but caused a quick increase (faster than MEPP- or Ca2+-hump) after the pause, whose magnitude increased with an increase in pause duration (<1 min), suggesting that Ca2+ entry-dependent inactivation, but not depriming process, explains the decay of the humps. The depriming process was seen by giving a much longer pause (>1 min). Thus, ryanodine receptors in frog motor nerve terminals are endowed with Ca2+ entry-dependent slow priming and fast inactivation mechanisms, as well as Ca2+ entry-dependent activation, and involved in asynchronous exocytosis. Physiological significance of CICR in presynaptic terminals was discussed.

Key words: intracellular calciumCa2+ influxryanodine receptortransmitter release


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Proc. Natl. Acad. Sci. USAHome page
T. Kenet, R. C. Froemke, C. E. Schreiner, I. N. Pessah, and M. M. Merzenich
Perinatal exposure to a noncoplanar polychlorinated biphenyl alters tonotopy, receptive fields, and plasticity in rat primary auditory cortex
PNAS, May 1, 2007; 104(18): 7646 - 7651.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
J. Talbot, J. N. Barrett, E. F. Barrett, and G. David
Stimulation-induced changes in NADH fluorescence and mitochondrial membrane potential in lizard motor nerve terminals
J. Physiol., March 15, 2007; 579(3): 783 - 798.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
S. Zissimopoulos, D. J. West, A. J. Williams, and F. A. Lai
Ryanodine receptor interaction with the SNARE-associated protein snapin
J. Cell Sci., June 1, 2006; 119(11): 2386 - 2397.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
G. R. J. Gordon and J. S. Bains
Noradrenaline Triggers Multivesicular Release at Glutamatergic Synapses in the Hypothalamus
J. Neurosci., December 7, 2005; 25(49): 11385 - 11395.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
K. Ouyang, H. Zheng, X. Qin, C. Zhang, D. Yang, X. Wang, C. Wu, Z. Zhou, and H. Cheng
Ca2+ sparks and secretion in dorsal root ganglion neurons
PNAS, August 23, 2005; 102(34): 12259 - 12264.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
Q. Liu, B. Chen, M. Yankova, D. K. Morest, E. Maryon, A. R. Hand, M. L. Nonet, and Z.-W. Wang
Presynaptic Ryanodine Receptors Are Required for Normal Quantal Size at the Caenorhabditis elegans Neuromuscular Junction
J. Neurosci., July 20, 2005; 25(29): 6745 - 6754.
[Abstract] [Full Text] [PDF]


Home page
Physiol. Rev.Home page
A. Verkhratsky
Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons
Physiol Rev, January 1, 2005; 85(1): 201 - 279.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
V. K. Unni, S. S. Zakharenko, L. Zablow, A. J. DeCostanzo, and S. A. Siegelbaum
Calcium Release from Presynaptic Ryanodine-Sensitive Stores Is Required for Long-Term Depression at Hippocampal CA3-CA3 Pyramidal Neuron Synapses
J. Neurosci., October 27, 2004; 24(43): 9612 - 9622.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
L. Oliveira, M. A. Timoteo, and P. Correia-de-Sa
Tetanic depression is overcome by tonic adenosine A2A receptor facilitation of L-type Ca2+ influx into rat motor nerve terminals
J. Physiol., October 1, 2004; 560(1): 157 - 168.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
R. Conti, Y. P. Tan, and I. Llano
Action Potential-Evoked and Ryanodine-Sensitive Spontaneous Ca2+ Transients at the Presynaptic Terminal of a Developing CNS Inhibitory Synapse
J. Neurosci., August 4, 2004; 24(31): 6946 - 6957.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
E. S. Wachman, R. E. Poage, J. R. Stiles, D. L. Farkas, and S. D. Meriney
Spatial Distribution of Calcium Entry Evoked by Single Action Potentials within the Presynaptic Active Zone
J. Neurosci., March 24, 2004; 24(12): 2877 - 2885.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
V. De Crescenzo, R. ZhuGe, C. Velazquez-Marrero, L. M. Lifshitz, E. Custer, J. Carmichael, F. A. Lai, R. A. Tuft, K. E. Fogarty, J. R. Lemos, et al.
Ca2+ Syntillas, Miniature Ca2+ Release Events in Terminals of Hypothalamic Neurons, Are Increased in Frequency by Depolarization in the Absence of Ca2+ Influx
J. Neurosci., February 4, 2004; 24(5): 1226 - 1235.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
M. Galante and A. Marty
Presynaptic Ryanodine-Sensitive Calcium Stores Contribute to Evoked Neurotransmitter Release at the Basket Cell-Purkinje Cell Synapse
J. Neurosci., December 3, 2003; 23(35): 11229 - 11234.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
J. Piriz, M. D. Rosato Siri, R. Pagani, and O. D. Uchitel
Nifedipine-Mediated Mobilization of Intracellular Calcium Stores Increases Spontaneous Neurotransmitter Release at Neonatal Rat Motor Nerve Terminals
J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 658 - 663.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
G. David and E. F Barrett
Mitochondrial Ca2+ uptake prevents desynchronization of quantal release and minimizes depletion during repetitive stimulation of mouse motor nerve terminals
J. Physiol., April 15, 2003; 548(2): 425 - 438.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
K. Yamaguchi, M. Tanaka, A. Mizoguchi, Y. Hirata, H. Ishizaki, K. Kaneko, J. Miyoshi, and Y. Takai
A GDP/GTP exchange protein for the Rab3 small G protein family up-regulates a postdocking step of synaptic exocytosis in central synapses
PNAS, October 29, 2002; 99(22): 14536 - 14541.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. Filipek, B. Jastrzebska, M. Nowotny, K. Kwiatkowska, M. Hetman, L. Surmacz, E. Wyroba, and J. Kuznicki
Ca2+-dependent Translocation of the Calcyclin-binding Protein in Neurons and Neuroblastoma NB-2a Cells
J. Biol. Chem., May 31, 2002; 277(23): 21103 - 21109.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
S. Suzuki, M. Osanai, N. Mitsumoto, T. Akita, K. Narita, H. Kijima, and K. Kuba
Ca2+-Dependent Ca2+ Clearance Via Mitochondrial Uptake and Plasmalemmal Extrusion in Frog Motor Nerve Terminals
J Neurophysiol, April 1, 2002; 87(4): 1816 - 1823.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
F.-M. Lu and K. Kuba
Synchronous and Asynchronous Exocytosis Induced by Subthreshold High K+ at Cs+-Loaded Terminals of Rat Hippocampal Neurons
J Neurophysiol, March 1, 2002; 87(3): 1222 - 1233.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
A. G. Carter, K. E. Vogt, K. A. Foster, and W. G. Regehr
Assessing the Role of Calcium-Induced Calcium Release in Short-Term Presynaptic Plasticity at Excitatory Central Synapses
J. Neurosci., January 1, 2002; 22(1): 21 - 28.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
G. Kang, O. G Chepurny, and G. G Holz
cAMP-regulated guanine nucleotide exchange factor II (Epac2) mediates Ca2+-induced Ca2+ release in INS-1 pancreatic {beta}-cells
J. Physiol., October 15, 2001; 536(2): 375 - 385.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
A. Castonguay and R. Robitaille
Differential Regulation of Transmitter Release by Presynaptic and Glial Ca2+ Internal Stores at the Neuromuscular Synapse
J. Neurosci., March 15, 2001; 21(6): 1911 - 1922.
[Abstract] [Full Text] [PDF]


Home page
J. Neurophysiol.Home page
J. K. Angleson and W. J. Betz
Intraterminal Ca2+ and Spontaneous Transmitter Release at the Frog Neuromuscular Junction
J Neurophysiol, January 1, 2001; 85(1): 287 - 294.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
C. W. Tsang, D. B. Elrick, and M. P. Charlton
alpha -Latrotoxin Releases Calcium in Frog Motor Nerve Terminals
J. Neurosci., December 1, 2000; 20(23): 8685 - 8692.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
G. David
Mitochondrial Clearance of Cytosolic Ca2+ in Stimulated Lizard Motor Nerve Terminals Proceeds without Progressive Elevation of Mitochondrial Matrix [Ca2+]
J. Neurosci., September 1, 1999; 19(17): 7495 - 7506.
[Abstract] [Full Text] [PDF]



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents