The Journal of General Physiology
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 1 May 2000. doi:10.1085/jgp.115.5.533
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 220K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grichtchenko, I. I.
Right arrow Articles by Boron, W. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grichtchenko, I. I.
Right arrow Articles by Boron, W. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© The Rockefeller University Press, 0022-1295/2000//533/ $5.00
Journal of General Physiology, Volume 115, Number 5, 2000

Original Article

Extracellular Hco3– Dependence of Electrogenic Na/Hco3 Cotransporters Cloned from Salamander and Rat Kidney

Irina I. Grichtchenkoa, Michael F. Romeroa, and Walter F. Borona

a From the Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520
Department of Cellular and Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520.203-785-4951

walter.boron{at}yale.edu

We studied the extracellular [HCOABSTRACT 3 –] dependence of two renal clones of the electrogenic Na/HCO3 cotransporter (NBC) heterologously expressed in Xenopus oocytes. We used microelectrodes to measure the change in membrane potential ({Delta}Vm) elicited by the NBC cloned from the kidney of the salamander Ambystoma tigrinum (akNBC) and by the NBC cloned from the kidney of rat (rkNBC). We used a two-electrode voltage clamp to measure the change in current ({Delta}I) elicited by rkNBC. Briefly exposing an NBC-expressing oocyte to HCOABSTRACT 3 –/CO2 (0.33–99 mM HCOABSTRACT 3–, pHo 7.5) elicited an immediate, DIDS (4,4-diisothiocyanatostilbene-2,2-disulfonic acid)-sensitive and Na+-dependent hyperpolarization (or outward current). In {Delta}Vm experiments, the apparent Km for HCOABSTRACT 3– of akNBC (10.6 mM) and rkNBC (10.8 mM) were similar. However, under voltage-clamp conditions, the apparent Km for HCOABSTRACT 3– of rkNBC was less (6.5 mM). Because it has been reported that SOABSTRACT 3=/HSO ABSTRACT 3– stimulates Na/HCO3 cotransport in renal membrane vesicles (a result that supports the existence of a COABSTRACT 3= binding site with which SOABSTRACT 3= interacts), we examined the effect of SOABSTRACT 3=/HSO ABSTRACT 3– on rkNBC. In voltage-clamp studies, we found that neither 33 mM SOABSTRACT 4= nor 33 mM SOABSTRACT 3 =/HSOABSTRACT 3– substantially affects the apparent Km for HCO ABSTRACT 3–. We also used microelectrodes to monitor intracellular pH (pHi) while exposing rkNBC-expressing oocytes to 3.3 mM HCOABSTRACT 3 –/0.5% CO2. We found that SO ABSTRACT 3=/HSOABSTRACT 3 – did not significantly affect the DIDS-sensitive component of the pHi recovery from the initial CO2 -induced acidification. We also monitored the rkNBC current while simultaneously varying [CO2]o, pHo, and [COABSTRACT 3=]o at a fixed [HCOABSTRACT 3–]o of 33 mM. A Michaelis-Menten equation poorly fitted the data expressed as current versus [COABSTRACT 3=]o . However, a pH titration curve nicely fitted the data expressed as current versus pHo. Thus, rkNBC expressed in Xenopus oocytes does not appear to interact with SOABSTRACT 3 =, HSOABSTRACT 3–, or COABSTRACT 3=.

Key Words: Xenopus oocytes • intracellular pH • extracellular pH • sulfite • carbonate

© 2000 The Rockefeller University Press


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. S. Yang, E. Kim, S. Lee, H. J. Park, D. S. Cooper, I. Rajbhandari, and I. Choi
Mutation of Aspartate 555 of the Sodium/Bicarbonate Transporter SLC4A4/NBCe1 Induces Chloride Transport
J. Biol. Chem., June 5, 2009; 284(23): 15970 - 15979.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. N. Chernova, A. K. Stewart, P. N. Barry, M. L. Jennings, and S. L. Alper
Mouse Ae1 E699Q mediates SO42-i/aniono exchange with [SO42-]i-dependent reversal of wild-type pHo sensitivity
Am J Physiol Cell Physiol, August 1, 2008; 295(2): C302 - C312.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Lu and W. F. Boron
Reversible and irreversible interactions of DIDS with the human electrogenic Na/HCO3 cotransporter NBCe1-A: role of lysines in the KKMIK motif of TM5
Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1787 - C1798.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
S. D. McAlear, X. Liu, J. B. Williams, C. M. McNicholas-Bevensee, and M. O. Bevensee
Electrogenic Na/HCO3 Cotransporter (NBCe1) Variants Expressed in Xenopus Oocytes: Functional Comparison and Roles of the Amino and Carboxy Termini
J. Gen. Physiol., May 30, 2006; 127(6): 639 - 658.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Dinour, M.-H. Chang, J.-i. Satoh, B. L. Smith, N. Angle, A. Knecht, I. Serban, E. J. Holtzman, and M. F. Romero
A Novel Missense Mutation in the Sodium Bicarbonate Cotransporter (NBCe1/SLC4A4) Causes Proximal Tubular Acidosis and Glaucoma through Ion Transport Defects
J. Biol. Chem., December 10, 2004; 279(50): 52238 - 52246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. B. Kintner, G. Su, B. Lenart, A. J. Ballard, J. W. Meyer, L. L. Ng, G. E. Shull, and D. Sun
Increased tolerance to oxygen and glucose deprivation in astrocytes from Na+/H+ exchanger isoform 1 null mice
Am J Physiol Cell Physiol, July 1, 2004; 287(1): C12 - C21.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
E. Odgaard, J. K. Jakobsen, S. Frische, J. Praetorius, S. Nielsen, C. Aalkjaer, and J. Leipziger
Basolateral Na+-dependent HCO3- transporter NBCn1-mediated HCO3- influx in rat medullary thick ascending limb
J. Physiol., February 15, 2004; 555(1): 205 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
I. Choi, L. Hu, J. D. Rojas, B. M. Schmitt, and W. F. Boron
Role of glycosylation in the renal electrogenic Na+-HCO-3 cotransporter (NBCe1)
Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1199 - F1206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. Hirata, T. Kaneko, T. Ono, T. Nakazato, N. Furukawa, S. Hasegawa, S. Wakabayashi, M. Shigekawa, M.-H. Chang, M. F. Romero, et al.
Mechanism of acid adaptation of a fish living in a pH 3.5 lake
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2003; 284(5): R1199 - R1212.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Jiang, I. I. Grichtchenko, W. F. Boron, and P. S. Aronson
Specificity of Anion Exchange Mediated by Mouse Slc26a6
J. Biol. Chem., September 6, 2002; 277(37): 33963 - 33967.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. I Grichtchenko, I. Choi, X. Zhong, P. Bray-Ward, J. M. Russell, and W. F. Boron
Cloning, Characterization, and Chromosomal Mapping of a Human Electroneutral Na+-driven Cl-HCO3 Exchanger
J. Biol. Chem., March 9, 2001; 276(11): 8358 - 8363.
[Abstract] [Full Text] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents