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1 Homomeric Glycine Receptor as a Function of the Number of Agonist Molecules Bound
Address correspondence to Marco Beato at his current address: Department of Pharmacology, University College London, Gower St., London WC1E 6BT. Tel.: (44) 20-7679 3765; Fax (44) 20-7679 7298; E-mail m.beato{at}ucl.ac.uk
The functional properties of rat homomeric 
1 glycine receptors were investigated using whole-cell and outside-out recording from human embryonic kidney cells transfected with rat 1 subunit cDNA. Whole-cell dose-response curves gave EC50 estimates between 30 and 120 µM and a Hill slope of 
3.3. Single channel recordings were obtained by steady-state application of glycine (0.3, 1, or 10 µM) to outside-out patches. Single channel conductances were mostly 60–90 pS, but smaller conductances of 40 pS were also seen (10% of the events) with a relative frequency that did not depend on agonist concentration. The time constants of the apparent open time distributions did not vary with agonist concentration, but short events were more frequent at low glycine concentrations. There was also evidence of a previously missed short-lived open state that was more common at lower glycine concentrations. The time constants for the different components of the burst length distributions were found to have similar values at different concentrations. Nevertheless, the mean burst length increased with increasing glycine. This was because the relative area of each burst-length component was concentration dependent and short bursts were favored at lower glycine concentrations. Durations of adjacent open and shut times were found to be strongly (negatively) correlated. Additionally, long bursts were made up of longer than average openings separated by short gaps, whereas short bursts usually consisted of single isolated short openings. The most plausible explanation for these findings is that long bursts are generated when a higher proportion of the five potential agonist binding sites on the receptor is occupied by glycine. On the basis of the concentration dependence and the intraburst structure we provide a preliminary kinetic scheme for the activation of the homomeric glycine receptor, in which any number of glycine molecules from one to five can open the channel, although not with equal efficiency.
Key Words: single channel • patch clamp • HEK • recombinant receptors • homomeric receptors
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