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¹ Biophysics Sector, International School for Advanced Studies, Trieste 34014, Italy
² INFM Unit, International School for Advanced Studies, Trieste 34014, Italy
³ Department of Physiology, Kazan Medical University, Kazan 420012, Russia
⁴ Department of Biophysics, Kazan State University, Kazan 42008, Russia

Address correspondence to A. Nistri, Biophysics Sector, International School for Advanced Studies, via Beirut 4, Trieste 34014, Italy. Fax (39) 040-3787528; E-mail: nistri{at}sissa.it

The mode of action of extracellular protons on ATP-gated P2X₂ receptors remains controversial as either enhancement or depression of ATP-mediated currents has been reported. By investigating, at different pH, the electrophysiological effect of ATP on P2X₂ receptors and complementing it with receptor modelling, the present study suggests a unified mechanism for both potentiation and inactivation of ATP receptors by protons. Our experiments on patch-clamped PC12 cells showed that, on the same cell, mild acidification potentiated currents induced by low ATP concentrations (<0.1 mM) and attenuated responses to high ATP concentrations (>1 mM) with emergence of current fading and rebound. To clarify the nature of the ATP/H⁺ interaction, we used the Ding and Sachs's "loop" receptor model which best describes the behavior of such receptors with two open states linked via one inactivated state. No effects by protons could be ascribed to H⁺-mediated open channel block. However, by assuming that protons facilitated binding of ATP to resting as well as open receptors, the model could closely replicate H⁺-induced potentiation of currents evoked by low ATP doses plus fading and rebound induced by high ATP doses. The latter phenomenon was due to receptor transition to the inactive state. The present data suggest that the high concentration of protons released with ATP (and catecholamines) from secretory vesicles may allow a dual action of H⁺ on P2X₂ receptors. This condition might also occur on P2X₂ receptors of central neurons exposed to low pH during ischemia.

Key Words: pH • potentiation • fading and rebound • modelling • purinergic

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