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ARTICLE |
Subunits in the Ca2+-activated K+ (BK) Channel
High conductance, calcium- and voltage-activated potassium (BK, MaxiK) channels are widely expressed in mammals. In some tissues, the biophysical properties of BK channels are highly affected by coexpression of regulatory (ß) subunits. The most remarkable effects of ß1 and ß2 subunits are an increase of the calcium sensitivity and the slow down of channel kinetics. However, the detailed characteristics of channels formed by
and ß1 or ß2 are dissimilar, the most remarkable difference being a reduction of the voltage sensitivity in the presence of ß1 but not ß2. Here we reveal the molecular regions in these ß subunits that determine their differential functional coupling with the pore-forming -subunit. We made chimeric constructs between ß1 and ß2 subunits, and BK channels formed by and chimeric ß subunits were expressed in Xenopus laevis oocytes. The electrophysiological characteristics of the resulting channels were determined using the patch clamp technique. Chimeric exchange of the different regions of the ß1 and ß2 subunits demonstrates that the NH3 and COOH termini are the most relevant regions in defining the behavior of either subunit. This strongly suggests that the intracellular domains are crucial for the fine tuning of the effects of these ß subunits. Moreover, the intracellular domains of ß1 are responsible for the reduction of the BK channel voltage dependence. This agrees with previous studies that suggested the intracellular regions of the -subunit to be the target of the modulation by the ß1-subunit.
P. Orio's present address is the Instituto de Neurociencias de Alicante, Universidad Miguel Hernandez-CSIC, 03550 Sant Joan d'Alacant, Spain.
P. Rojas's present address is the Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110
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