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The Journal of General Physiology, Vol 13, 469-476, Copyright © 1930 by The Rockefeller University Press


ARTICLE

PROTEIN COAGULATION AND ITS REVERSAL

THE PREPARATION OF INSOLUBLE GLOBIN, SOLUBLE GLOBIN AND HEME



M. L. Anson 1 and A. E. Mirsky 1

1 From the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J., and the Hospital of The Rockefeller Institute for Medical Research, New York

1. By a procedure involving the use of acid acetone hemoglobin may be rapidly separated into a precipitate of denatured globin and an acetone solution of heme.

2. By neutralization procedures the denatured globin may be largely converted into a soluble, apparently native form which can combine with heme to form hemoglobin.

3. The heme may be obtained in acetone-free, slightly alkaline solution without the use of strong alkali which may modify the heme.

Accepted on January 20, 1930


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