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¹ Center for Neuroscience and Department of Neurology, University of California, Davis, CA 95616
² St. Vincent's Institute, Fitzroy, Victoria 3065, Australia

Correspondence to Tsung-Yu Chen: tycchen{at}ucdavis.edu

The CLC-1 Cl^– channel is abundantly expressed on the plasma membrane of muscle cells, and the membrane potential of muscle cells is largely controlled by the activity of this Cl^– channel. Previous studies showed that low intracellular pH increases the overall open probability of recombinant CLC-1 channels in various expression systems. Low intracellular pH, however, is known to inhibit the Cl^– conductance on the native muscle membrane, contradicting the findings from the recombinant CLC-1 channels in expressed systems. Here we show that in the presence of physiological concentrations of ATP, reduction of the intracellular pH indeed inhibits the expressed CLC-1, mostly by decreasing the open probability of the common gate of the channel.

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