The Journal of General Physiology
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The Journal of General Physiology, Vol 24, 459-465, Copyright © 1941 by The Rockefeller University Press

ARTICLE

A NEW METHOD FOR THE STUDY OF DIFFUSION OF BIOLOGICALLY ACTIVE MATERIAL

Jaques Bourdillon 1

1 From the Laboratories of the International Health Division of The Rockefeller Foundation, New York

A simple diffusion apparatus has been described in which a layer of solution is allowed to diffuse upward into a layer of solvent. Accurate sampling is performed at various heights and the concentration of the samples is determined.

The method has been illustrated with a determination of the diffusion constant of crystalline catalase, which was found to be 3.1 x 10–7 cm.2/sec. at 4°C. The method should be especially suited to the study of biological substances endowed with specific activity and which cannot be obtained in pure solution.

 Submitted on October 22, 1940


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