The Journal of General Physiology
Cell MicroControls
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The Journal of General Physiology, Vol 68, 197-210, Copyright © 1976 by The Rockefeller University Press

ARTICLES

Biochemical isolation and physiological identification of the egg- laying hormone in Aplysia californica

S Arch, P Earley and T Smock

It has been determined that the bag cells of Aplysia californica produce two polypeptide species that comigrate on electrophoretic gels containing sodium dodecyl sulfate. By this separation procedure both species can be assigned a molecular weight of approximately 6,000. One of these molecules has an Rf of 0.65 on alkaline discontinuous electrophoresis gels, an isoelectric point at pH 4.8, a gel filtration molecular weight of approximately 12,000, and has no known biological function. The other does not enter alkaline disk gels, has an isoelectric point at approximately pH 9.3, shows a gel filtration molecular weight consistent with that determined by SDS gel electrophoresis, and is the egg-laying hormone.
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P Brownell and E Mayeri
Prolonged inhibition of neurons by neuroendocrine cells in Aplysia
Science, April 27, 1979; 204(4391): 417 - 420.
[Abstract] [PDF]

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HAROLD. M. PINSKER and F. E. DUDEK
Bag Cell Control of Egg Laying in Freely Behaving Aplysia
Science, July 29, 1977; 197(4302): 490 - 493.
[Abstract] [PDF]


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