The Journal of General Physiology
89 NORTH - Illumination Systems for Fluorescence Imaging
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 1387K)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JGP
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cala, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cala, P. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

The Journal of General Physiology, Vol 76, Issue 6 683-708, Copyright © 1980 by Society of General Physiologists

JOURNAL ARTICLE

Volume regulation by Amphiuma red blood cells. The membrane potential and its implications regarding the nature of the ion-flux pathways

P. M. Cala
Department of Human Physiology, University of California School of Medicine, Davis 95616, USA.

After osmotic perturbation, the red blood cells of Amphiuma exhibited a volume-regulatory response that returned cell volume back to or toward control values. After osmotic swelling, cell-volume regulation (regulatory volume decrease; RVD) resulted from net cellular loss of K, Cl, and osmotically obliged H2O. In contrast, the volume-regulatory response to osmotic shrinkage (regulatory volume increase; RVI) was characterized by net cellular uptake of Na, Cl, and H2O. The net K and Na fluxes characteristic of RVD and RVI are increased by 1-2 orders of magnitude above those observed in studies of volume-static control cells. The cell membrane potential of volume-regulating and volume-static cells was measured by impalement with glass microelectrodes. The information gained from the electrical and ion-flux studies led to the conclusion that the ion fluxes responsible for cell-volume regulation proceed via electrically silent pathways. Furthermore, it was observed that Na fluxes during RVI were profoundly sensitive to medium [HCO3] and that during RVI the medium becomes more acid, whereas alkaline shifts in the suspension medium accompany RVD. The experimental observations are explained by a model featuring obligatorily coupled alkali metal-H and Cl-HCO3 exchangers. The anion- and cation-exchange pathways are separate and distinct yet functionally coupled via the net flux of H. As a result of the operation of such pathways, net alkali metal, Cl, and H2O fluxes proceed in the same direction, whereas H and HCO3 fluxes are cyclic. Data also are presented that suggest that the ion-flux pathways responsible for cell-volume regulation are not activated by changes in cell volume per se but by some event associated with osmotic perturbation, such as changes in intracellular pH.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Ortiz-Acevedo, R. R. Rigor, H. M. Maldonado, and P. M. Cala
Coordinated control of volume regulatory Na+/H+ and K+/H+ exchange pathways in Amphiuma red blood cells
Am J Physiol Cell Physiol, March 1, 2010; 298(3): C510 - C520.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. K. Hoffmann, I. H. Lambert, and S. F. Pedersen
Physiology of Cell Volume Regulation in Vertebrates
Physiol Rev, January 1, 2009; 89(1): 193 - 277.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. H. Herok, T. J. Millar, P. J. Anderton, and D. K. Martin
Role of Chloride Channels in Regulating the Volume of Acinar Cells of the Rabbit Superior Lacrimal Gland
Invest. Ophthalmol. Vis. Sci., December 1, 2008; 49(12): 5517 - 5525.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Ortiz-Acevedo, R. R. Rigor, H. M. Maldonado, and P. M. Cala
Activation of Na+/H+ and K+/H+ exchange by calyculin A in Amphiuma tridactylum red blood cells: implications for the control of volume-induced ion flux activity
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1316 - C1325.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. F. Pedersen, S. A. King, E. B. Nygaard, R. R. Rigor, and P. M. Cala
NHE1 Inhibition by Amiloride- and Benzoylguanidine-type Compounds: INHIBITOR BINDING LOCI DEDUCED FROM CHIMERAS OF NHE1 HOMOLOGUES WITH ENDOGENOUS DIFFERENCES IN INHIBITOR SENSITIVITY
J. Biol. Chem., July 6, 2007; 282(27): 19716 - 19727.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
K. Kristensen, P. Koldkjaer, M. Berenbrink, and T. Wang
Oxygen-sensitive regulatory volume increase and Na transport in red blood cells from the cane toad, Bufo marinus
J. Exp. Biol., July 1, 2007; 210(13): 2290 - 2299.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Miyazaki, A. Shiozaki, N. Niisato, and Y. Marunaka
Physiological significance of hypotonicity-induced regulatory volume decrease: reduction in intracellular Cl- concentration acting as an intracellular signaling
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1411 - F1417.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Rivera, L. De Franceschi, L. L. Peters, P. Gascard, N. Mohandas, and C. Brugnara
Effect of complete protein 4.1R deficiency on ion transport properties of murine erythrocytes
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C880 - C886.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. F. Pedersen, M. E. O'Donnell, S. E. Anderson, and P. M. Cala
Physiology and pathophysiology of Na+/H+ exchange and Na+-K+-2Cl- cotransport in the heart, brain, and blood
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R1 - R25.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. F. Hoffman and J. P. Geibel
Fluorescent imaging of Cl- in Amphiuma red blood cells: How the nuclear exclusion of Cl- affects the plasma membrane potential
PNAS, January 18, 2005; 102(3): 921 - 926.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. F. Pedersen, S. A. King, R. R. Rigor, Z. Zhuang, J. M. Warren, and P. M. Cala
Molecular cloning of NHE1 from winter flounder RBCs: activation by osmotic shrinkage, cAMP, and calyculin A
Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1561 - C1576.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
O. Aharonovitz, A. Kapus, K. Szaszi, N. Coady-Osberg, T. Jancelewicz, J. Orlowski, and S. Grinstein
Modulation of Na+/H+ exchange activity by Cl{-}
Am J Physiol Cell Physiol, July 1, 2001; 281(1): C133 - C141.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. A. McLean, S. Zia, F. A. Gorin, and P. M. Cala
Cloning and expression of the Na+/H+ exchanger from Amphiuma RBCs: resemblance to mammalian NHE1
Am J Physiol Cell Physiol, May 1, 1999; 276(5): C1025 - C1037.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
E. M. Hogan, B. A. Davis, and W. F. Boron
Intracellular Cl- Dependence of Na-H Exchange in Barnacle Muscle Fibers under Normotonic and Hypertonic Conditions
J. Gen. Physiol., November 1, 1997; 110(5): 629 - 639.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. Fisher, B. Persson, and K. Spring
Epithelial cell volume regulation: bicarbonate dependence
Science, December 18, 1981; 214(4527): 1357 - 1359.
[Abstract] [PDF]


  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents