|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 76, Issue 6 751-762, Copyright © 1980 by Society of General Physiologists
JOURNAL ARTICLE |
S. Nakajima and A. Gilai
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
Isolated single (Xenopus) muscle fibers were stained with a non-permeant potential-probing dye, merocyanine rhodanine (WW375) or merocyanine oxazolone (NK2367). When the fiber was massively stimulated, an absorption change (wave a), which seemed to reflect the action potential, occurred. Simultaneous recording of optical changes and intracellular action potentials revealed that the time-course of wave a was slower than the action potential: the peak of wave a was attained at 1 ms, and the peak of action potential was reached at 0.5 ms after the stimulation. This difference suggests that wave a represents the potential changes of the whole tubular membrane and the surface membrane, whereas the action potential represents a surface potential change. This idea was substantiated by recording absorption signals preferentially from the surface membrane by recording the absorption changes at the edge of the fiber. Wave a obtained by this method was as quick as the intracellular action potential. The value of radial conduction velocity of action potential along the T system, calculated by comparing the action potential with wave a, was 6.4 cm/s at 24.5 degrees C, in fair agreement with Gonzalez-Serratos (1971. J. Physiol. [Lond.]. 212:777-799). The shape of wave a suggests the existence of an access delay (a conduction delay at the orifice of the T system) of 130 microseconds.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. P. Cairns, A. J. Taberner, and D. S. Loiselle Changes of surface and t-tubular membrane excitability during fatigue with repeated tetani in isolated mouse fast- and slow-twitch muscle J Appl Physiol, January 1, 2009; 106(1): 101 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Cairns, E. R. Chin, and J.-M. Renaud Stimulation pulse characteristics and electrode configuration determine site of excitation in isolated mammalian skeletal muscle: implications for fatigue J Appl Physiol, July 1, 2007; 103(1): 359 - 368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. Head, H. H. Patel, D. M. Roth, N. C. Lai, I. R. Niesman, M. G. Farquhar, and P. A. Insel G-protein-coupled Receptor Signaling Components Localize in Both Sarcolemmal and Intracellular Caveolin-3-associated Microdomains in Adult Cardiac Myocytes J. Biol. Chem., September 2, 2005; 280(35): 31036 - 31044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G S Posterino, G D Lamb, and D G Stephenson Twitch and tetanic force responses and longitudinal propagation of action potentials in skinned skeletal muscle fibres of the rat J. Physiol., August 15, 2000; 527(1): 131 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Soeller and M. B. Cannell Examination of the Transverse Tubular System in Living Cardiac Rat Myocytes by 2-Photon Microscopy and Digital Image–Processing Techniques Circ. Res., February 19, 1999; 84(3): 266 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.M. Baylor and S. Hollingworth Model of Sarcomeric Ca2+ Movements, Including ATP Ca2+ Binding and Diffusion, during Activation of Frog Skeletal Muscle J. Gen. Physiol., September 1, 1998; 112(3): 297 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
