The Journal of General Physiology
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The Journal of General Physiology, Vol 77, 549-570, Copyright © 1981 by The Rockefeller University Press

ARTICLES

Osmotic water permeability of human red cells

TC Terwilliger and AK Solomon

The osmotic water permeability of human red cells has been reexamined with a stopped-flow device and a new perturbation technique. Small osmotic gradients are used to minimize the systematic error caused by nonlinearities in the relationship between cell volume and light scattering. Corrections are then made for residual systematic error. Our results show that the hydraulic conductivity, Lp, is essentially independent of the direction of water flow and of osmolality in the range 184-365 mosM. the mean value of Lp obtained obtained was 1.8 +/- 0.1 (SEM) X 10-11 cm3 dyne -1 s-1.
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