|
|
|
|
|
|
|
||
The Journal of General Physiology, Vol 83, 875-899, Copyright © 1984 by The Rockefeller University Press
ARTICLES |
BA Hughes, SS Miller and TE Machen
A modified version of a capacitance probe technique has been used to measure fluid transport across the isolated retinal pigment epithelium (RPE)-choroid of the bullfrog. The accuracy of this measurement is 0.5- 1.0 nl/min. Experiments carried out in the absence of external osmotic or hydrostatic gradients show that the RPE-choroid transports fluid from the retinal to the choroid side of the tissue at a rate of approximately 10 nl/min (4-6 microliters/cm2 X h). Net fluid absorption (Jv) was abolished within 10 min by the mitochondrial uncoupler 2,4- dinitrophenol. It was also inhibited (70%) by the removal of bicarbonate from the bulk solutions bathing the tissue. Ouabain caused a slow decrease in Jv (no effect at 10 min, 70% at 3 h), which indicates that RPE fluid transport is not directly coupled to the activity of the Na-K pump located at the apical membrane of this epithelium. In contrast to ouabain, cyclic AMP (cAMP) produced a quick decrease in Jv (84% within 5 min). Radioisotope experiments in the open circuit show that cAMP stimulated secretory fluxes of Na and Cl, which accounted for the observed cAMP-induced decrease in Jv. The direction of net fluid absorption, the magnitudes of the net ionic fluxes in the open circuit, and the dependence of Jv on external bicarbonate concentration strongly suggest that fluid absorption is generated primarily by the active absorption of bicarbonate.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Adijanto, T. Banzon, S. Jalickee, N. S. Wang, and S. S. Miller CO2-induced ion and fluid transport in human retinal pigment epithelium J. Gen. Physiol., May 25, 2009; 133(6): 603 - 622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Reigada, W. Lu, and C. H. Mitchell Glutamate acts at NMDA receptors on fresh bovine and on cultured human retinal pigment epithelial cells to trigger release of ATP J. Physiol., September 15, 2006; 575(3): 707 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Strauss The Retinal Pigment Epithelium in Visual Function Physiol Rev, July 1, 2005; 85(3): 845 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Fischmeister and H. C. Hartzell Volume sensitivity of the bestrophin family of chloride channels J. Physiol., January 15, 2005; 562(2): 477 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Reigada and C. H. Mitchell Release of ATP from retinal pigment epithelial cells involves both CFTR and vesicular transport Am J Physiol Cell Physiol, January 1, 2005; 288(1): C132 - C140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Irokawa, M. E. Krouse, N. S. Joo, J. V. Wu, and J. J. Wine A "virtual gland" method for quantifying epithelial fluid secretion Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L784 - L793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Blaug, J. Rymer, S. Jalickee, and S. S. Miller P2 purinoceptors regulate calcium-activated chloride and fluid transport in 31EG4 mammary epithelia Am J Physiol Cell Physiol, April 1, 2003; 284(4): C897 - C909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Maminishkis, S. Jalickee, S. A. Blaug, J. Rymer, B. R. Yerxa, W. M. Peterson, and S. S. Miller The P2Y2 Receptor Agonist INS37217 Stimulates RPE Fluid Transport In Vitro and Retinal Reattachment in Rat Invest. Ophthalmol. Vis. Sci., November 1, 2002; 43(11): 3555 - 3566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Blaug, K. Hybiske, J. Cohn, G. L. Firestone, T. E. Machen, and S. S. Miller ENaC- and CFTR-dependent ion and fluid transport in mammary epithelia Am J Physiol Cell Physiol, August 1, 2001; 281(2): C633 - C648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rymer, S. S. Miller, and J. L. Edelman Epinephrine-Induced Increases in [Ca2+]in and KCl-Coupled Fluid Absorption in Bovine RPE Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1921 - 1929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-F. Arndt, A. Sari, M. Ferre, E. Parrat, D. Courtas, J. De Seze, J.-C. Hache, and R. Matran Electrophysiological Effects of Corticosteroids on the Retinal Pigment Epithelium Invest. Ophthalmol. Vis. Sci., February 1, 2001; 42(2): 472 - 475. [Abstract] [Full Text]
|
|
|
|
|
|
N. K. Wills, T. Weng, L. Mo, H. L. Hellmich, A. Yu, T. Wang, S. Buchheit, and B. F. Godley Chloride Channel Expression in Cultured Human Fetal RPE Cells: Response to Oxidative Stress Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4247 - 4255. [Abstract] [Full Text]
|
|
|
|
|
|
S. N. Uyekubo, H. Fischer, A. Maminishkis, B. Illek, S. S. Miller, and J. H. Widdicombe cAMP-dependent absorption of chloride across airway epithelium Am J Physiol Lung Cell Mol Physiol, December 1, 1998; 275(6): L1219 - L1227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C King-Smith, P Chen, D Garcia, H Rey, and B Burnside Calcium-independent regulation of pigment granule aggregation and dispersion in teleost retinal pigment epithelial cells J. Cell Sci., January 1, 1996; 109(1): 33 - 43. [Abstract] [PDF]
|
|
|
|
 |
|
 M. F. Marmor and X.-Y. Yao The Metabolic Dependency of Retinal Adhesion in Rabbit and Primate Arch Ophthalmol, February 1, 1995; 113(2): 232 - 238. [Abstract] [PDF]
|
|
|
|
 |
|
 C Jiang, W. Finkbeiner, J. Widdicombe, P. McCray Jr, and S. Miller Altered fluid transport across airway epithelium in cystic fibrosis Science, October 15, 1993; 262(5132): 424 - 427. [Abstract] [PDF]
|
|
|
|
|
|
M. F. Marmor and A. Negi Pharmacologic Modification of Subretinal Fluid Absorption in the Rabbit Eye Arch Ophthalmol, November 1, 1986; 104(11): 1674 - 1677. [Abstract] [PDF]
|
|
|
|
|
|
S. Tsuboi and J. E. Pederson Experimental Retinal Detachment: XI. Furosemide-Inhibitable Fluid Absorption Across Retinal Pigment Epithelium In Vivo Arch Ophthalmol, April 1, 1986; 104(4): 602 - 603. [Abstract] [PDF]
|
|
|
|
|
|
S. Tsuboi and J. E. Pederson Experimental Retinal Detachment: X. Effect of Acetazolamide on Vitreous Fluorescein Disappearance Arch Ophthalmol, October 1, 1985; 103(10): 1557 - 1558. [Abstract] [PDF]
|
|
|
|
