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The Journal of General Physiology, Vol 92, 145-159, Copyright © 1988 by The Rockefeller University Press


ARTICLES

Muscarinic activation of ionic currents measured by a new whole-cell recording method

R Horn and A Marty
Laboratoire de Neurobiologie, Ecole Normale Superieure, Paris, France.

A new method is described as an alternative to whole-cell recording in order to prevent "wash-out" of the muscarinic response to acetylcholine (ACh) in rat lacrimal gland cells. The membrane of a cell-attached patch is permeabilized by nystatin in the patch pipette, thus providing electrical continuity between the pipette and the cytoplasm of the cell without the loss or alteration of cytoplasmic compounds necessary for the maintenance of the response to ACh. With normal whole-cell recording in these cells, the response to ACh, seen as the activation of Ca-activated K and Cl currents, lasts for approximately 5 min. With the nystatin method, the response is not diminished after 1 h. Nystatin, applied extracellularly, is shown to cause a rapid and reversible increase of membrane conductance to cations. In the absence of wash-out, we were able to obtain dose-response curves for the effect of ACh on Ca-activated K currents. An increase of [ACh] caused an increase in the K current, with apparent saturation at concentrations above approximately 1 microM ACh. The delay between ACh application and the activation of K current was inversely related to [ACh] and reached a minimum value of 0.7-1.0 s at high [ACh].
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Am. J. Physiol. Cell Physiol.Home page
J. E. Bradley, U. A. Anderson, S. M. Woolsey, K. D. Thornbury, N. G. McHale, and M. A. Hollywood
Characterization of T-type calcium current and its contribution to electrical activity in rabbit urethra
Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1078 - C1088.
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J. Physiol.Home page
M. Craven, G. P. Sergeant, M. A. Hollywood, N. G. McHale, and K. D. Thornbury
Modulation of spontaneous Ca2+-activated Cl- currents in the rabbit corpus cavernosum by the nitric oxide-cGMP pathway
J. Physiol., April 15, 2004; 556(2): 495 - 506.
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Am. J. Physiol. Renal Physiol.Home page
S. H. Boese, O. Aziz, N. L. Simmons, and M. A. Gray
Kinetics and regulation of a Ca2+-activated Cl- conductance in mouse renal inner medullary collecting duct cells
Am J Physiol Renal Physiol, April 1, 2004; 286(4): F682 - F692.
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Circ. Res.Home page
M. E. Diaz, S. C. O'Neill, and D. A. Eisner
Sarcoplasmic Reticulum Calcium Content Fluctuation Is the Key to Cardiac Alternans
Circ. Res., March 19, 2004; 94(5): 650 - 656.
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J. Physiol.Home page
S. A. Locknar, K. L. Barstow, J. D. Tompkins, L. A. Merriam, and R. L. Parsons
Calcium-induced calcium release regulates action potential generation in guinea-pig sympathetic neurones
J. Physiol., March 15, 2004; 555(3): 627 - 635.
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JGPHome page
A. Priel and S. D. Silberberg
Mechanism of Ivermectin Facilitation of Human P2X4 Receptor Channels
J. Gen. Physiol., February 23, 2004; 123(3): 281 - 293.
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J. Neurosci.Home page
G. Cheron, D. Gall, L. Servais, B. Dan, R. Maex, and S. N. Schiffmann
Inactivation of Calcium-Binding Protein Genes Induces 160 Hz Oscillations in the Cerebellar Cortex of Alert Mice
J. Neurosci., January 14, 2004; 24(2): 434 - 441.
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J. Neurophysiol.Home page
S. C. Lee, Y. Hayashida, and A. T. Ishida
Availability of Low-Threshold Ca2+ Current in Retinal Ganglion Cells
J Neurophysiol, December 1, 2003; 90(6): 3888 - 3901.
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J. Neurophysiol.Home page
L. Catacuzzeno, B. Fioretti, and F. Franciolini
Voltage-Gated Outward K Currents in Frog Saccular Hair Cells
J Neurophysiol, December 1, 2003; 90(6): 3688 - 3701.
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J. Physiol.Home page
M. Fernandez-Velasco, N. Goren, G. Benito, J. Blanco-Rivero, L. Bosca, and C. Delgado
Regional distribution of hyperpolarization-activated current (If) and hyperpolarization-activated cyclic nucleotide-gated channel mRNA expression in ventricular cells from control and hypertrophied rat hearts
J. Physiol., December 1, 2003; 553(2): 395 - 405.
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CirculationHome page
M. L. Walker, X. Wan, G. E. Kirsch, and D. S. Rosenbaum
Hysteresis Effect Implicates Calcium Cycling as a Mechanism of Repolarization Alternans
Circulation, November 25, 2003; 108(21): 2704 - 2709.
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J. Neurosci.Home page
J. Verweij, E. P. Hornstein, and J. L. Schnapf
Surround Antagonism in Macaque Cone Photoreceptors
J. Neurosci., November 12, 2003; 23(32): 10249 - 10257.
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J. Neurophysiol.Home page
R. Tyzio, A. Ivanov, C. Bernard, G. L. Holmes, Y. Ben-Ari, and R. Khazipov
Membrane Potential of CA3 Hippocampal Pyramidal Cells During Postnatal Development
J Neurophysiol, November 1, 2003; 90(5): 2964 - 2972.
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J. Neurosci.Home page
D. Gall, C. Roussel, I. Susa, E. D'Angelo, P. Rossi, B. Bearzatto, M. C. Galas, D. Blum, S. Schurmans, and S. N. Schiffmann
Altered Neuronal Excitability in Cerebellar Granule Cells of Mice Lacking Calretinin
J. Neurosci., October 15, 2003; 23(28): 9320 - 9327.
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J. Neurophysiol.Home page
T. Sickmann and C. Alzheimer
Short-Term Desensitization of G-Protein-Activated, Inwardly Rectifying K+ (GIRK) Currents in Pyramidal Neurons of Rat Neocortex
J Neurophysiol, October 1, 2003; 90(4): 2494 - 2503.
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Am. J. Physiol. Cell Physiol.Home page
S. Ben-Tabou De-Leon, E. Blotnick, and I. Nussinovitch
Effects of osmotic swelling on voltage-gated calcium channel currents in rat anterior pituitary cells
Am J Physiol Cell Physiol, October 1, 2003; 285(4): C840 - C852.
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J. Physiol.Home page
G. M Herrera, M. J Pozo, P. Zvara, G. V Petkov, C. T Bond, J. P Adelman, and M. T Nelson
Urinary bladder instability induced by selective suppression of the murine small conductance calcium-activated potassium (SK3) channel
J. Physiol., September 15, 2003; 551(3): 893 - 903.
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J. Physiol.Home page
M. Zhang, I. M Fearon, H. Zhong, and C. A Nurse
Presynaptic modulation of rat arterial chemoreceptor function by 5-HT: role of K+ channel inhibition via protein kinase C
J. Physiol., September 15, 2003; 551(3): 825 - 842.
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J. Neurosci.Home page
C. C. Hegg, D. Greenwood, W. Huang, P. Han, and M. T. Lucero
Activation of Purinergic Receptor Subtypes Modulates Odor Sensitivity
J. Neurosci., September 10, 2003; 23(23): 8291 - 8301.
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J. Neurophysiol.Home page
J. C. Holt, M. Lioudyno, and P. S. Guth
A Pharmacologically Distinct Nicotinic ACh Receptor Is Found in a Subset of Frog Semicircular Canal Hair Cells
J Neurophysiol, September 1, 2003; 90(3): 1526 - 1536.
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J. Neurophysiol.Home page
F. Beker, M. Weber, R. H. A. Fink, and D. J. Adams
Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca2+ in Rat Intracardiac Ganglion Neurons
J Neurophysiol, September 1, 2003; 90(3): 1956 - 1964.
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J. Physiol.Home page
M A Hollywood, S. Woolsey, I K Walsh, P F Keane, N G McHale, and K D Thornbury
T- and L-type Ca2+ Currents in Freshly Dispersed Smooth Muscle Cells from the Human Proximal Urethra
J. Physiol., August 1, 2003; 550(3): 753 - 764.
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J. Physiol.Home page
X.-P. Chu, X.-M. Zhu, W.-L. Wei, G.-H. Li, R. P Simon, J. F MacDonald, and Z.-G. Xiong
Acidosis decreases low Ca2+ -induced neuronal excitation by inhibiting the activity of calcium-sensing cation channels in cultured mouse hippocampal neurons
J. Physiol., July 15, 2003; 550(2): 385 - 399.
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Am. J. Physiol. Heart Circ. Physiol.Home page
M. T. Lin, D. A. Hessinger, W. J. Pearce, and L. D. Longo
Developmental differences in Ca2+-activated K+ channel activity in ovine basilar artery
Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H701 - H709.
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J. Neurophysiol.Home page
H. Bao, W. H. Wong, J. M. Goldberg, and R. A. Eatock
Voltage-Gated Calcium Channel Currents in Type I and Type II Hair Cells Isolated From the Rat Crista
J Neurophysiol, July 1, 2003; 90(1): 155 - 164.
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J. Physiol.Home page
K. S Thorneloe and M. T Nelson
Properties and molecular basis of the mouse urinary bladder voltage-gated K+ current
J. Physiol., May 15, 2003; 549(1): 65 - 74.
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J. Neurosci.Home page
C. E. Armstrong-Gold and F. Rieke
Bandpass Filtering at the Rod to Second-Order Cell Synapse in Salamander (Ambystoma tigrinum) Retina
J. Neurosci., May 1, 2003; 23(9): 3796 - 3806.
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J. Biol. Chem.Home page
C.-X. Fan, X.-K. Chen, C. Zhang, L.-X. Wang, K.-L. Duan, L.-L. He, Y. Cao, S.-Y. Liu, M.-N. Zhong, C. Ulens, et al.
A Novel Conotoxin from Conus betulinus, kappa -BtX, Unique in Cysteine Pattern and in Function as a Specific BK Channel Modulator
J. Biol. Chem., April 4, 2003; 278(15): 12624 - 12633.
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J. Neurosci.Home page
S. Watanabe, T. Inoue, and Y. Kirino
Contribution of Excitatory Chloride Conductance in the Determination of the Direction of Traveling Waves in an Olfactory Center
J. Neurosci., April 1, 2003; 23(7): 2932 - 2938.
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J. Physiol.Home page
X.-L. Lou, X. Yu, X.-K. Chen, K.-L. Duan, L.-M. He, A.-L. Qu, T. Xu, and Z. Zhou
Na+ channel inactivation: a comparative study between pancreatic islet {beta}-cells and adrenal chromaffin cells in rat
J. Physiol., April 1, 2003; 548(1): 191 - 202.
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J. Physiol.Home page
H. Ishibashi, M. Umezu, I.-S. Jang, Y. Ito, and N. Akaike
{alpha}1-Adrenoceptor-activated cation currents in neurones acutely isolated from rat cardiac parasympathetic ganglia
J. Physiol., April 1, 2003; 548(1): 111 - 120.
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Am. J. Physiol. Cell Physiol.Home page
S. J. Liu, R. H. Kennedy, M. H. Creer, and J. McHowat
Alterations in Ca2+ cycling by lysoplasmenylcholine in adult rabbit ventricular myocytes
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C826 - C838.
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DiabetesHome page
S. G. Straub, J. Mulvaney-Musa, H. Yajima, G. A. Weiland, and G. W.G. Sharp
Stimulation of Insulin Secretion by Denatonium, One of the Most Bitter-Tasting Substances Known
Diabetes, February 1, 2003; 52(2): 356 - 364.
[Abstract] [Full Text] [PDF]



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