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The Journal of General Physiology, Vol 92, 145-159, Copyright © 1988 by The Rockefeller University Press
ARTICLES |
R Horn and A Marty
Laboratoire de Neurobiologie, Ecole Normale Superieure, Paris, France.
A new method is described as an alternative to whole-cell recording in order to prevent "wash-out" of the muscarinic response to acetylcholine (ACh) in rat lacrimal gland cells. The membrane of a cell-attached patch is permeabilized by nystatin in the patch pipette, thus providing electrical continuity between the pipette and the cytoplasm of the cell without the loss or alteration of cytoplasmic compounds necessary for the maintenance of the response to ACh. With normal whole-cell recording in these cells, the response to ACh, seen as the activation of Ca-activated K and Cl currents, lasts for approximately 5 min. With the nystatin method, the response is not diminished after 1 h. Nystatin, applied extracellularly, is shown to cause a rapid and reversible increase of membrane conductance to cations. In the absence of wash-out, we were able to obtain dose-response curves for the effect of ACh on Ca-activated K currents. An increase of [ACh] caused an increase in the K current, with apparent saturation at concentrations above approximately 1 microM ACh. The delay between ACh application and the activation of K current was inversely related to [ACh] and reached a minimum value of 0.7-1.0 s at high [ACh].
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D. Gall, C. Roussel, I. Susa, E. D'Angelo, P. Rossi, B. Bearzatto, M. C. Galas, D. Blum, S. Schurmans, and S. N. Schiffmann Altered Neuronal Excitability in Cerebellar Granule Cells of Mice Lacking Calretinin J. Neurosci., October 15, 2003; 23(28): 9320 - 9327. [Abstract] [Full Text] [PDF] |
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T. Sickmann and C. Alzheimer Short-Term Desensitization of G-Protein-Activated, Inwardly Rectifying K+ (GIRK) Currents in Pyramidal Neurons of Rat Neocortex J Neurophysiol, October 1, 2003; 90(4): 2494 - 2503. [Abstract] [Full Text] [PDF] |
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S. Ben-Tabou De-Leon, E. Blotnick, and I. Nussinovitch Effects of osmotic swelling on voltage-gated calcium channel currents in rat anterior pituitary cells Am J Physiol Cell Physiol, October 1, 2003; 285(4): C840 - C852. [Abstract] [Full Text] [PDF] |
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G. M Herrera, M. J Pozo, P. Zvara, G. V Petkov, C. T Bond, J. P Adelman, and M. T Nelson Urinary bladder instability induced by selective suppression of the murine small conductance calcium-activated potassium (SK3) channel J. Physiol., September 15, 2003; 551(3): 893 - 903. [Abstract] [Full Text] [PDF] |
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M. Zhang, I. M Fearon, H. Zhong, and C. A Nurse Presynaptic modulation of rat arterial chemoreceptor function by 5-HT: role of K+ channel inhibition via protein kinase C J. Physiol., September 15, 2003; 551(3): 825 - 842. [Abstract] [Full Text] [PDF] |
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C. C. Hegg, D. Greenwood, W. Huang, P. Han, and M. T. Lucero Activation of Purinergic Receptor Subtypes Modulates Odor Sensitivity J. Neurosci., September 10, 2003; 23(23): 8291 - 8301. [Abstract] [Full Text] [PDF] |
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J. C. Holt, M. Lioudyno, and P. S. Guth A Pharmacologically Distinct Nicotinic ACh Receptor Is Found in a Subset of Frog Semicircular Canal Hair Cells J Neurophysiol, September 1, 2003; 90(3): 1526 - 1536. [Abstract] [Full Text] [PDF] |
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F. Beker, M. Weber, R. H. A. Fink, and D. J. Adams Muscarinic and Nicotinic ACh Receptor Activation Differentially Mobilize Ca2+ in Rat Intracardiac Ganglion Neurons J Neurophysiol, September 1, 2003; 90(3): 1956 - 1964. [Abstract] [Full Text] [PDF] |
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M A Hollywood, S. Woolsey, I K Walsh, P F Keane, N G McHale, and K D Thornbury T- and L-type Ca2+ Currents in Freshly Dispersed Smooth Muscle Cells from the Human Proximal Urethra J. Physiol., August 1, 2003; 550(3): 753 - 764. [Abstract] [Full Text] [PDF] |
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X.-P. Chu, X.-M. Zhu, W.-L. Wei, G.-H. Li, R. P Simon, J. F MacDonald, and Z.-G. Xiong Acidosis decreases low Ca2+ -induced neuronal excitation by inhibiting the activity of calcium-sensing cation channels in cultured mouse hippocampal neurons J. Physiol., July 15, 2003; 550(2): 385 - 399. [Abstract] [Full Text] [PDF] |
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M. T. Lin, D. A. Hessinger, W. J. Pearce, and L. D. Longo Developmental differences in Ca2+-activated K+ channel activity in ovine basilar artery Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H701 - H709. [Abstract] [Full Text] [PDF] |
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H. Bao, W. H. Wong, J. M. Goldberg, and R. A. Eatock Voltage-Gated Calcium Channel Currents in Type I and Type II Hair Cells Isolated From the Rat Crista J Neurophysiol, July 1, 2003; 90(1): 155 - 164. [Abstract] [Full Text] [PDF] |
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K. S Thorneloe and M. T Nelson Properties and molecular basis of the mouse urinary bladder voltage-gated K+ current J. Physiol., May 15, 2003; 549(1): 65 - 74. [Abstract] [Full Text] [PDF] |
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C. E. Armstrong-Gold and F. Rieke Bandpass Filtering at the Rod to Second-Order Cell Synapse in Salamander (Ambystoma tigrinum) Retina J. Neurosci., May 1, 2003; 23(9): 3796 - 3806. [Abstract] [Full Text] [PDF] |
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C.-X. Fan, X.-K. Chen, C. Zhang, L.-X. Wang, K.-L. Duan, L.-L. He, Y. Cao, S.-Y. Liu, M.-N. Zhong, C. Ulens, et al. A Novel Conotoxin from Conus betulinus, kappa -BtX, Unique in Cysteine Pattern and in Function as a Specific BK Channel Modulator J. Biol. Chem., April 4, 2003; 278(15): 12624 - 12633. [Abstract] [Full Text] [PDF] |
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S. Watanabe, T. Inoue, and Y. Kirino Contribution of Excitatory Chloride Conductance in the Determination of the Direction of Traveling Waves in an Olfactory Center J. Neurosci., April 1, 2003; 23(7): 2932 - 2938. [Abstract] [Full Text] [PDF] |
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X.-L. Lou, X. Yu, X.-K. Chen, K.-L. Duan, L.-M. He, A.-L. Qu, T. Xu, and Z. Zhou Na+ channel inactivation: a comparative study between pancreatic islet {beta}-cells and adrenal chromaffin cells in rat J. Physiol., April 1, 2003; 548(1): 191 - 202. [Abstract] [Full Text] [PDF] |
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H. Ishibashi, M. Umezu, I.-S. Jang, Y. Ito, and N. Akaike {alpha}1-Adrenoceptor-activated cation currents in neurones acutely isolated from rat cardiac parasympathetic ganglia J. Physiol., April 1, 2003; 548(1): 111 - 120. [Abstract] [Full Text] [PDF] |
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S. J. Liu, R. H. Kennedy, M. H. Creer, and J. McHowat Alterations in Ca2+ cycling by lysoplasmenylcholine in adult rabbit ventricular myocytes Am J Physiol Cell Physiol, April 1, 2003; 284(4): C826 - C838. [Abstract] [Full Text] [PDF] |
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S. G. Straub, J. Mulvaney-Musa, H. Yajima, G. A. Weiland, and G. W.G. Sharp Stimulation of Insulin Secretion by Denatonium, One of the Most Bitter-Tasting Substances Known Diabetes, February 1, 2003; 52(2): 356 - 364. [Abstract] [Full Text] [PDF] |
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