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The Journal of General Physiology, Vol 92, 685-711, Copyright © 1988 by The Rockefeller University Press
ARTICLES |
JR Sachs
Department of Medicine, State University of New York, Stony Brook 11794.
K influx into resealed human red cell ghosts increases when the ghosts are swollen. The influx demonstrates properties similar to volume- sensitive K fluxes present in other cells. The influx is, for the most part, insensitive to the nature of the major intracellular cation and therefore is not a K-K exchange. The influx is much greater when the major anion is Cl than when the major anion is NO3; Cl stimulates the flux and, at constant Cl, NO3 inhibits it. Increase in the influx rate is rapid when shrunken ghosts are swollen or when NO3 is replaced by Cl. The volume-sensitive K influx requires intracellular MgATP at low concentrations, and ATP cannot be replaced by nonhydrolyzable ATP analogues. The volume-sensitive influx is inhibited by Mg2+ and by high concentrations of vanadate, but is stimulated by low concentrations of vanadate. It is not modified by cAMP, the removal of Ca2+ by EGTA, substances that activate protein kinase C, or by inhibition of phosphatidylinositol kinase. The influx is inhibited by neomycin and by trifluoperazine.
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  J R Sachs and D W Martin Role of polyamine structure in inhibition of K+-Cl- cotransport in human red cell ghosts J. Physiol., November 1, 1999; 520(3): 723 - 735. [Abstract] [Full Text] [PDF]
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