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Original Article

Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655

Address correspondence to Michael J. Sanderson, Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655. Fax: (508) 856-5997; E-mail: michael.sanderson{at}umassmed.edu

	ABSTRACT

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The Ca²⁺ signaling and contractility of airway smooth muscle cells (SMCs) were investigated with confocal microscopy in murine lung slices (75-µm thick) that maintained the in situ organization of the airways and the contractility of the SMCs for at least 5 d. 10–500 nM acetylcholine (ACH) induced a contraction of the airway lumen and a transient increase in [Ca²⁺]_i in individual SMCs that subsequently declined to initiate multiple intracellular Ca²⁺ oscillations. These Ca²⁺ oscillations spread as Ca²⁺ waves through the SMCs at 48 µm/s. The magnitude of the airway contraction, the initial Ca²⁺ transient, and the frequency of the subsequent Ca²⁺ oscillations were all concentration-dependent. In a Ca²⁺-free solution, ACH induced a similar Ca²⁺ response, except that the Ca²⁺ oscillations ceased after 1–1.5 min. Incubation with thapsigargin, xestospongin, or ryanodine inhibited the ACH-induced Ca²⁺ signaling. A comparison of airway contraction with the ACH-induced Ca²⁺ response of the SMCs revealed that the onset of airway contraction correlated with the initial Ca²⁺ transient, and that sustained airway contraction correlated with the occurrence of the Ca²⁺ oscillations. Buffering intracellular Ca²⁺ with BAPTA prohibited Ca²⁺ signaling and airway contraction, indicating a Ca²⁺-dependent pathway. Cessation of the Ca²⁺ oscillations, induced by ACH-esterase, halothane, or the absence of extracellular Ca²⁺ resulted in a relaxation of the airway. The concentration dependence of the airway contraction matched the concentration dependence of the increased frequency of the Ca²⁺ oscillations. These results indicate that Ca²⁺ oscillations, induced by ACH in murine bronchial SMCs, are generated by Ca²⁺ release from the SR involving IP₃- and ryanodine receptors, and are required to maintain airway contraction.

Key Words: asthma • hyper-reactivity • confocal microscopy • Ca²⁺ oscillations • frequency modulation

	INTRODUCTION

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Our understanding of hyper-reactivity associated with asthma, which is the narrowing of airways induced by smooth muscle cell (SMC)^* contraction, is assimilated from investigations of either whole airways (e.g., lung function tests or isolated airways) or from investigations of isolated single SMCs. Although studies with whole airways provide data about airway contraction, they provide little information regarding the underlying biological processes. By contrast, studies of isolated SMCs provide information regarding the cellular processes, but little information regarding the functional properties of the airway. The use of isolated SMCs has some additional drawbacks, including the tendency of the cells to de-differentiate and to lose contractility. For example, the response of cultured airway SMCs to stimulation via the muscarinic receptor subtype M3 that mediates SMC contraction by activating PLC and increasing intracellular Ca²⁺ concentration ([Ca²⁺]_i) is reduced (Widdop et al., 1993; Hall and Kotlikoff, 1995).

To address some of these problems, we have refined the experimental use of lung slices. Lung slices previously have been used to study a variety of lung responses including bronchial contractility (Dandurand et al., 1993; Martin et al., 1996, 2000a,b, 2001; Salerno et al., 1996; Minshall et al., 1997; Duguet et al., 2000), vascular responses (Held et al., 1999), mucociliary function (Kurosawa et al., 1995), and allergic responses mediated by inflammatory cells (Dandurand et al., 1994; Wohlsen et al., 2001). Although most studies used video microscopy to document changes in airway size, the thickness of the slices precluded the observation of single cells. For example, Placke and Fisher (1987) and Dandurand et al. (1993) cut slices by hand that were 1,000–2,000-µm and 500–1,000-µm thick, respectively. Although Martin et al. (1996) reduced the slice thickness to 250 µm using a tissue slicer, these slices remained unsuitable for single cell studies. With the aid of a vibratome tissue slicer, we have further reduced the slice thickness to 75 µm and, using confocal microscopy, we were able to study individual cells in the lung slice.

Agonist-induced increases in [Ca²⁺]_i frequently results in the stimulation of intracellular Ca²⁺ oscillations, and the idea arising from this correlation is that frequency-modulated Ca²⁺ signals regulate cellular activities (for reviews see Berridge et al., 2000; Bootman et al., 2001). In isolated tracheal SMCs, acetylcholine (ACH) has been found to induce an initial Ca²⁺ transient that was associated with an increase in SMC tension (Shieh et al., 1991). In the continued presence of ACH, this transient Ca²⁺ increase appeared to be followed by a reduced, but sustained, plateau in [Ca²⁺]_i that was sufficient for the maintenance of tension. Subsequent studies have revealed that the ACH-induced Ca²⁺ transient was actually followed by Ca²⁺ oscillations (Liu and Farley, 1996; Sims et al., 1996; Prakash et al., 1997, 2000; Sieck et al., 1997). However, these studies were performed on isolated tracheal SMCs, and the relationship of these Ca²⁺ transients and Ca²⁺ oscillations to airway caliber was unknown. Therefore, the correlation of the Ca²⁺ signaling in bronchial SMCs to airway contraction was the primary aim of the present study.

Cellular mechanisms of agonist-induced Ca²⁺ signaling in SMCs involve G-protein mediated activation of PLC, which hydrolyses phosphatidylinositol 4,5 bisphosphate to form diacylglycerol and inositol 1,4,5 trisphosphate (IP₃). IP₃ binds to specific receptors (IP₃R) within the SR and releases Ca²⁺ from the SR (for review see Bootman et al., 2001). However, the contribution of CICR from the SR via RYR and Ca²⁺ influx across the cell membrane varies among SMCs with respect to cell type and species (Savineau and Marthan, 2000). Therefore, the present study is also focused on the mechanisms involved in ACH-induced Ca²⁺ oscillations in murine SMCs of airways distal to the trachea.

In this study, we present a unique system using thin lung slices and confocal microscopy to study both the cellular Ca²⁺ signaling events in airway SMCs and the impact that this has on airway contraction. From our data, we suggest that ACH-induced Ca²⁺ oscillations in airway SMCs are generated by Ca²⁺ release from the SR involving IP₃R and RYR and that these Ca²⁺ oscillations are responsible for sustained SMC contraction and the associated reduced airway caliber. We also hypothesize that the magnitude of the sustained contraction may be regulated by the frequency of the Ca²⁺ oscillations.

	MATERIALS AND METHODS

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Materials
Cell culture reagents and plasticware were obtained from Invitrogen Life Technologies. Other reagents were obtained from Sigma-Aldrich unless otherwise stated. In most cases, the Hanks' balanced salt solution (sHBSS) was supplemented with 25 mMHEPES buffer but lacked phenol red, unless otherwise stated.

Lung Slices
To cut thin lung slices, it was first necessary to stiffen and stabilize the soft lung tissue. This was achieved by inflating the lungs with liquid agarose that was subsequently induced to gel by reducing the temperature. A solution of 4% agarose (type VII-A: low gelling temperature) in distilled water was prepared at 60°C, cooled to 37°C, and mixed with 2x concentrated sHBSS with phenol red to give a 2% agarose-sHBSS solution at 37°C. Male BALB/C inbred strain mice (42–77 d old; Charles River Breeding Labs) were killed by intraperitoneal injection (12 ml/kg bodyweight Nembutal). The fur was rinsed with ethanol and the trachea was exposed. Sternotomy was performed and the chest wall was removed. Care was taken not to injure the lungs. To fill the lungs with agarose, the trachea was cannulated using an intravenous catheter system (model 20G Intima; Becton Dickinson). The inner needle was removed and the outer intravenous tube was secured with suture thread (Dexon II, 4–0; Davis and Geck). To allow the air trapped in the intravenous tube to escape, the tube was initially clamped next to the trachea and an unattached 20-gauge needle was inserted into the tube, distal to the clamp. Once the tube was filled with agarose-sHBSS using a syringe, the clamp was removed and the lungs were slowly inflated with agarose-sHBSS (1 ml). To prevent premature gelling of the agarose, the mouse and associated equipment were kept warm with a heat lamp. Subsequently, 0.1–0.2 ml of air was injected to flush the agarose-sHBSS out of the airways. The intravenous tube was clamped again to prevent leakage. The lungs were rinsed with 4°C sHBSS and the mouse was placed at 4°C for 15 min. The lungs were removed, placed in 4°C sHBSS, and cooled for an additional 15 min to ensure the complete gelling of the agarose within the lungs. After isolating single lung lobes, slices of 75-µm thickness were cut at 4°C in sHBSS with a tissue slicer (model EMS-4000; Electron Microscopy Sciences). Up to eight slices were placed in one petri dish and cultured by floating them in 2 ml of DMEM supplemented with 10% FBS and antibiotics and antimycotics at 37°C in 10% CO₂. The actual slice thickness was measured using a position servo controller (model LVPZT E-662; Physik Instrumente) mounted on a confocal microscope.

Immunohistochemistry
Slices were washed in sHBSS, fixed for 5 min in 100% acetone, and washed in sHBSS containing 10% FBS. Antibodies were diluted 1:100 in sHBSS with 10% FBS. The slices were incubated for 1 h at room temperature with monoclonal mouse anti–-smooth muscle actin (product number A-2547; Sigma-Aldrich) or monoclonal mouse anti–pan cytokeratin (product number C-2562) antibodies. Slices were washed in sHBSS with 10% FBS, incubated for 1 h at room temperature with FITC-conjugated anti–mouse IgG (1:100 in sHBSS with 10% FBS), embedded in anti-fade solution (SlowFade; Molecular Probes), and inspected on a confocal microscope.

Measurement of Airway Contraction or Relaxation
Experiments were performed on lung slices containing airways cut in cross-section. Care was taken to select airways with a lumen that was free of agarose and completely lined by epithelial cells showing ciliary activity. The slices were placed on cover glasses within a custom made Plexiglas chamber, held in position by a nylon mesh (CMN-300-B; Small Parts Inc.) and observed with an inverted microscope (model Diaphot 300; Nikon) using a 20x objective. The bathing solution for all experiments was sHBSS. Phase-contrast images were recorded using a digital high speed CCD camera (model TM-6710; Pulnix America), a digital camera interface ("Road Runner"; BitFlow Inc.) and image acquisition software ("Video Savant"; IO Industries Inc.). Frames were captured in time-lapse (1 frame/s), stored in TIF stacks of several hundred frames, and analyzed on a Pentium III computer using the image analysis software "Scion" (Scion Corporation; free download: www.scioncorp.com). After calibrating the image dimensions and setting an appropriate threshold to distinguish the lumen from the surrounding tissue, the area of the airway lumen was measured with respect to time by pixel summing. To emulate a line-scanning analysis of airway contraction, a single row of pixels, aligned across the lumen of the airway, was extracted from each frame within the image stack and aligned sequentially into a single image. Each analysis was performed using custom written macros.

Measurements of Intracellular Free Ca²⁺ Concentration
Slices were loaded for 1 h at room temperature with 20 µM Oregon green (Molecular Probes) in sHBSS containing 0.2% pluronic (Pluronic F-127; Calbiochem), 100 µM sulfobromophthalein, and 3 mg/ml ascorbic acid. After loading, the slices were incubated for at least 30 min in sHBSS containing 100 µM sulfobromophthalein and 3 mg/ml ascorbic acid to allow for complete dye deesterification. The bath solution for all experiments was sHBSS containing 3 mg/ml ascorbic acid (antioxidant to reduce bleaching).

Confocal microscopy was performed with a custom-built microscope (Sanderson and Parker, 2002) based on an inverted microscope. Briefly, the 488-nm line from an argon laser is scanned across the specimen with two oscillating mirrors (for X- and Y-scan). The resultant fluorescence (>510 nm) is detected by a photomultiplier tube. A video frame capture board digitizes the photomultiplier signal to form an image that is recorded to hard disc using the recording software "Video Savant." Images were recorded in time-lapse (1 or 4 frames/s, 420 pixels x 400 lines) or at high speed (60 frames/s, 420 pixels x 200 lines) for the detection of rapid changes. Regions of interest (ROI) of 10 x 10 pixels were defined in single SMCs. Average fluorescence intensities of an ROI were obtained, frame-by-frame, using a custom written macro. Bleach correction was calculated from the bleaching rate during a period of 15–30 s before the addition of drugs. Raw data were corrected with the bleach correction before a rolling average of three frames was calculated. Final fluorescence values were expressed as a fluorescence ratio (F/F₀) normalized to the initial fluorescence (F₀). Because the confocal system was not capable of recording transmitted phase-contrast images simultaneously with confocal fluorescence images, airway contraction was analyzed by measuring the area of the part of the airway lumen that was visible in the confocal images.

Drug Application
ACH chloride, ACH-esterase, and halothane were dissolved in sHBSS. A 10x solution was diluted to the final concentration by addition to the volume of the bath solution (e.g., 10 µl of 10^-5 M ACH was added to 90 µl bath solution for a final concentration of 10^-6 M ACH). BAPTA-AM, xestospongin (Calbiochem), ryanodine, and thapsigargin were dissolved in DMSO and diluted in sHBSS to the final concentrations used.

Statistics
Statistical analysis was performed using the one way repeated measurements analysis of variance (all pair-wise multiple comparison procedures = Student-Newman-Keuls method). A P value of P < 0.05 was considered statistically significant.

	RESULTS

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Lung Slice Morphology and Properties
Agarose-filled murine lungs were cut into slices with a thickness of 73.3 ± 22.8 µm (mean ± SD, n = 10) using a vibratome tissue slicer. The slices were maintained by floating them in culture medium to prevent adhesion to the dish and were found, as judged by conventional phase-contrast microscopy, to retain the normal morphological appearance and arrangement of the alveoli, blood vessels, and airways. Airways, cut in cross-section, could be easily identified and distinguished from blood vessels and alveoli based on a number of criteria. In contrast to blood vessels, the lumens of the airways were devoid of blood cells and were lined with cuboidal epithelial cells (ECs) that had clearly visible beating cilia (Fig. 1). The airways were relatively thick-walled in comparison to the numerous alveoli that formed thin-walled structures surrounding air spaces. Because of the extensive branching of the respiratory tract, airways were frequently cut at oblique angles. In the extreme case, a longitudinal section of an airway could be obtained. However, the same criteria, as described above, were applied to identify these airways.

View larger version (77K): [in this window] [in a new window]   FIGURE 1. Phase-contrast images of a lung slice, 75-µm thick, cut from agarose-filled murine lungs. (A) A low magnification image of a single airway in the lung slice. The airway is cut in cross-section and has a clearly visible lumen (AL). Several adjacent thin-walled alveoli (AV) are also evident. (B) A higher magnification view of the area outlined by the rectangle in A showing the epithelial cells (EC) that line the airway lumen. (C) A higher magnification of the area outlined by the rectangle in B showing the cilia (Ci) of the epithelial cells facing toward the airway lumen. Bars: (A) 50 µm; (B) 20 µm; (C) 8 µm.

View larger version (78K): [in this window] [in a new window]   FIGURE 2. Confocal microscopy images of immunocytochemical stainings of airways in lung slices using FITC-conjugated secondary antibodies. (A) The wall of an airway, cut in cross-section, after staining with anti–-actin antibodies. Several smooth muscle cells (SMC) stained brightly, show an elongated fusiform cell shape, and are in close spatial proximity to epithelial cells (EC) and airway lumen (AL). (B) A slice stained with anti–pan cytokeratin antibodies showing the typical cobblestone-like appearance of epithelial cells. In this case, the plane of the section has passed obliquely through the airway to provide near longitudinal section at the top left. Bars: 15 µm.

Morphology and contractility remained stable for at least 5 d in culture. Ciliary activity showed no apparent change for at least 10 d. After 10 d (without a change of culture medium), contractility decreased markedly and ciliary activity began to slow. As a result, experiments were performed only on slices 2–5 d old.

For the first few days after slice preparation, spontaneous airway contractions could be randomly observed (Fig. 3, before the addition of ACH). Unfortunately, a full systematic investigation of this phenomenon was hindered by its unpredictability. In lung slices of some mice, spontaneous contractions occurred in many airways, whereas in slices from other mice, no spontaneous activity could be observed at all. Similarly, the contractions ranged from "twitches" of parts of the airway wall without substantial change in airway lumen to contractions of the whole circumference, resulting in reduction of airway lumen of up to 50%. However, confocal microscopy did reveal that the spontaneous contractions occurred simultaneously with Ca²⁺ transients in SMCs. Whereas exposure to ACH-esterase (n = 4) or apyrase (n = 3) did not stop the contractions, exposure to a Ca²⁺-free solution immediately stopped the spontaneous contractions (n = 4; unpublished data). These results suggest that an influx of external Ca²⁺ may play a role in the mechanisms responsible for spontaneous contractions. A detailed analysis of this phenomenon is beyond the scope of this work and will be the focus of future studies.

View larger version (80K): [in this window] [in a new window]   FIGURE 3. The change in the cross-sectional area of an airway in response to acetylcholine (ACH). An airway was monitored with phase-contrast microscopy and recorded in time-lapse at 1 frame/s in response to 1 µM ACH and 85 U/ml ACH-Esterase. (top, 1–4) Four images of the airway at different times (indicated by the dotted lines) showing the change in lumen size over 15 min. (middle) The cross-sectional area of the lumen was calculated and plotted against time. The airway displayed a number of spontaneous contractions followed by a large contraction in response to the application of ACH (bar). 1 µM ACH induced an airway contraction of 50%. An initial steep phase of fast narrowing was followed by an asymptotic phase. The addition of 85 U/ml ACH-Esterase (bar) led to a short transient contraction followed by a relaxation of the airway back to baseline. (bottom) A line-scanning analysis of the airway responses induced by ACH and ACH-Esterase. Gray values of a single line across each image (black line in phase images 1–4) were sequentially aligned (vertically) to produce a 1-D temporal image of the activity. Trace is representative of eight experiments performed in eight different airways in eight different slices from two different mice. Bar: 50 µm.

View larger version (17K): [in this window] [in a new window]   FIGURE 4. The concentration-response curve of ACH-induced airway contraction. Airways in lung slices were exposed to ACH and the minimum cross-sectional area was determined. A decrease in cross-sectional area occurs in the range of 10–500 nM. Concentrations of 1 µM or higher did not further decrease the minimum cross-sectional area, resulting in a plateau of contraction equal to 80%. Each point represents five to eight experiments (mean ± SD) using a different airway in a different slice for each experiment. For each point, slices from at least two different mice were used.

The Ca²⁺ response to ACH consisted of an initial transient increase in [Ca²⁺]_i followed by Ca²⁺ oscillations (Figs. 5 and 6 A). By contrast, the [Ca²⁺]_i in ECs did not change in response to ACH. The addition of sHBSS alone had no effect. To evaluate the concentration dependence of the Ca²⁺ response, five SMCs in different slices were exposed to 10^-8, 10^-6, and 10^-3 M ACH; a washout and a 20-min recovery period was allowed between each ACH exposure. The increase in [Ca²⁺]_i during the initial Ca²⁺ transient (the percent difference between the F/F₀ measured immediately before and at maximum value of the Ca²⁺ transient) was concentration-dependent from 10 nM to 1 mM (8.9 ± 15.2% for 10^-8 M, 33 ± 31.8% for 10^-6 M, and 73.1 ± 19% for 10^-3 M; increase in percent of baseline, mean ± SD, P < 0.05, Fig. 6 B). The frequency of the Ca²⁺ oscillations (the average period of 10 sequential oscillations) at 10^-6 M (25.2 ± 16.2 min^-1, mean ± SD) was higher than at 10^-8 M (16.1 ± 17.5, P < 0.05) but did not differ between 10^-6 and 10^-3 M (28.4 ± 23.3; Fig. 6 C).

View larger version (61K): [in this window] [in a new window]   FIGURE 5. (A) A series of confocal pseudocolor images showing that epithelial cells (EC) separate the airway lumen (AL) from several adjacent smooth muscle cells (asterisks). In response to 1 µM ACH, the [Ca2+]i is increased in the SMCs but not in the epithelial cells. Each SMC responds with a different time course. Time after the addition of ACH is indicated under each panel. White lines indicate the estimated cell boundaries. (B) A line-scan analysis of the effect of ACH. Intensity values from a single row (black line in image "500 ms") for each image recorded during 25 s were aligned into a single image. The arrow indicates the addition of ACH. ACH-induced airway contraction occurred simultaneously with the [Ca2+]i increase.

View larger version (18K): [in this window] [in a new window]   FIGURE 6. (A) Ca2+ signaling induced by ACH in airway SMCs in lung slices. ROIs were defined in SMCs and Ca2+ changes in response to 1 µM ACH (bar) expressed as a fluorescence ratio. The Ca2+ response consisted of an initial Ca2+ transient, followed by Ca2+ oscillations. (B and C) Concentration dependence of the Ca2+ response to ACH. Five SMCs in airways of different slices were exposed to 10-8, 10-6, and 10-3 M ACH with a washout and a 20-min recovery period between each exposure (*, P < 0.05). (B) The magnitude of the Ca2+ increase of the initial Ca2+ transient (the percent difference between the F/F0 measured immediately before and at maximum value of the Ca2+ transient) was concentration-dependent. (C) The frequency of the oscillations (the average period of 10 sequential oscillations) at 10-6 M was higher than at 10-8 M, but did not differ between 10-6 and 10-3 M.

View larger version (28K): [in this window] [in a new window]   FIGURE 7. (A) Ca2+ oscillations as intracellular Ca2+ waves. Using confocal microscopy, ACH-induced Ca2+ oscillations were recorded at 60 frames/s. Two ROIs in the same SMC (21.6 µm apart) were analyzed, and ACH-induced Ca2+ oscillations displayed simultaneously. The Ca2+ oscillations of the two ROIs were phase-shifted, indicating a Ca2+ wave propagating through the cell. The temporal difference between the ROIs was 0.49 ± 0.11 s, giving a propagating velocity of the Ca2+ wave of 45.7 ± 11.1 µm/s for this cell (n = 5 consecutive oscillations, mean ± SD). Three similar experiments in three different SMCs in three different slices revealed an average propagating velocity of 47.6 ± 9.2 µm/s. (B) Ca2+ oscillations in ROIs in two adjacent cells were recorded and displayed simultaneously. The oscillations were asynchronous, indicating independent Ca2+ responses to ACH.

View larger version (22K): [in this window] [in a new window]   FIGURE 8. Mechanisms of ACH-induced Ca2+ oscillations. Traces are representative of four to five experiments performed in different airways of different slices obtained from at least two different mice. (A) In Ca2+-free solution containing 5 mM EGTA, ACH-induced Ca2+ oscillations were initiated, but ceased after 1–1.5 min. (B) Incubation with 10 µM thapsigargin for 30 min abolished the Ca2+ response to ACH. (C) After a 45-min incubation with 10 µM xestospongin, no Ca2+ response to ACH could be detected. (D) Incubation with 200 µM ryanodine for 45 min also prevented the ACH-induced Ca2+ response.

View larger version (25K): [in this window] [in a new window]   FIGURE 9. Correlation of ACH-induced Ca2+ signaling and airway contraction. ROIs were defined in single SMCs, and the Ca2+ changes in response to 10 µM ACH were expressed as a fluorescence ratio (top trace). The change in the part of the cross-sectional area of the airway that was visible in the confocal images was measured and displayed simultaneously as the delta area (bottom trace). The initial Ca2+ transient correlates with the airway contraction. The occurrence of the Ca2+ oscillations correlates with the maintenance of the reduced airway lumen. Representative traces of five experiments of five different airways in five different slices obtained from two different mice are shown.

View larger version (30K): [in this window] [in a new window]   FIGURE 10. Ca2+ oscillations maintain airway contraction. ROIs were defined in single SMCs and the Ca2+ changes in response to ACH expressed as fluorescence ratio (top). The change in the part of the cross-sectional area of the airway that was visible in the confocal images was measured and displayed simultaneously as the delta area (bottom). Traces are representative of four to five experiments performed in different airways of different slices obtained from at least two different mice. (A) Buffering intracellular Ca2+ by incubation with 1 mM BAPTA-AM for 1 h prevented both, the Ca2+ signaling and the airway contraction in response to 10 µM ACH (bar). (B) During ongoing ACH-induced Ca2+ oscillations, the addition of 85 U/ml ACH-Esterase (bar) abolished the Ca2+ oscillations without substantially changing baseline [Ca2+]i and relaxed the airway. (C) In a Ca2+-free solution containing 5 mM EGTA, the addition of 10 µM ACH (bar) induced Ca2+ oscillations and airway contraction. However, after 1–1.5 min, the Ca2+ oscillations ceased and the airway relaxed. (D) 1.7 mM halothane abolished ongoing Ca2+ oscillations and simultaneously relaxed the airway.

	DISCUSSION

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To address some of these difficulties, we have refined the use of lung slices. We found that SMCs within the slice maintained the ability to perform multiple contractions for at least 5 d. These slices required minimal maintenance and appeared healthy without any special culturing conditions. Healthy lung slices also were indicated by the presence of active cilia on the airway epithelial cells. The morphology of the slice appeared, as discernible with the light microscope, unaltered with the airway epithelial cells remaining cuboidal and the SMCs remaining elongated. Obviously, some connective tissue has been disrupted at the surface of the slices, but for cells in the middle of a slice, which are accessible with confocal microscopy, their environment approaches that of in situ. Thus, slices would appear ideal for the investigation of interactions, not only between SMCs, but also between SMCs and epithelial cells as well as the activity of individual SMCs and the response of the associated airway.

Despite these advantages, the lung slice is not without some difficulties. For example, lung slices do not adhere to the substrate and are easily displaced by experimental protocols. We have found that a piece nylon mesh can be used to hold slices in position. Lung slices also are not easily loaded with indicator dyes. This may be because the surface area of the cells in a slice that is accessible to the dye is significantly reduced by surrounding cells and connective tissue, but an increase in dye concentration and incubation time and the addition of an organic anion transporter blocker appeared to compensate for a low dye-loading rate. The process of obtaining a slice may be considered traumatic, but the process of cell isolation involves an enzymatic modification of the cell surface, a loss of cell contacts, and a lack of the connective tissue forces that mediate relaxation.

We used a higher concentration of agarose (2%) to stiffen the compliant lung tissue as compared with Dandurand et al. (1993) and Martin et al. (1996), 1% and 0.75%, respectively. The stiffer lung and the use of a precision tissue slicer or vibratome, in place of manual or hand cutting, enabled us to obtain thin slices that allow the study of single cells within the slice with confocal microscopy.

ACH-induced Airway Contraction
Many studies on airway contraction have been performed using methacholine (MCH), a chemical analogue of ACH acting via muscarinic receptors that may vary in subtype selectivity depending on species and tissue (Tulic et al., 1999). The choice to use MCH may have been based on the slower metabolism of MCH and the widespread use of MCH in clinical lung function tests. However, we choose to use ACH because ACH is readily metabolized by esterases for reversibility of experiments, contracts airway smooth muscle cells through activation of muscarinic receptors (Hall, 2000), narrows airways in lung parenchymal strips (Salerno et al., 1996) and induces Ca²⁺ oscillations in isolated tracheal SMCs (Liu and Farley, 1996; Sims et al., 1996; Prakash et al., 1997, 2000; Sieck et al., 1997).

A concentration dependence of ACH-induced airway contraction was observed in the range of 10–500 nM. At concentrations >500 nM, ACH induced maximal airway contraction, which was, on average, 80% of the initial area. However, these results contrast to MCH-induced narrowing in thicker lung slices where increasing concentrations of MCH led to a reduction in airway lumen to <20% and complete airway closure in up to one third of the airways (Dandurand et al., 1993; Martin et al., 1996; Minshall et al., 1997). One explanation for this difference could be our use of a higher concentration of agarose to fill the lungs as compared with the previous studies because of our effort to cut thinner slices for the use with confocal microscopy. This could have increased the stiffness of the surrounding agarose-filled tissue. However, Dandurand et al. (1993) reported that 16% of the airways closed in rat lung slices when using 2% agarose. The slices used in the present study were thinner and likely to have fewer SMCs per airway. Consequently, the number of SMCs may have been insufficient to generate the force required to narrow or close airways to the same extent.

ACH-induced Ca²⁺ Signaling
The Ca²⁺ response of airway SMCs to ACH showed a biphasic pattern consisting of an initial transient followed by Ca²⁺ oscillations. ACH-induced Ca²⁺ oscillations also have been reported to occur in isolated tracheal SMCs (Liu and Farley, 1996; Sims et al., 1996; Prakash et al., 1997, 2000; Sieck et al., 1997). The oscillation frequencies in this study (20–30 per minute) were comparable to those found for isolated tracheal SMCs (Pabelick et al., 2001b) although Sieck et al. (1997) reported slower frequencies for bronchial SMCs. We also found, like Prakash et al. (2000), a concentration dependence of the frequency of the oscillations. This concentration dependence matched that of airway contraction, and this will be addressed later.

The Ca²⁺ oscillations were found to spread through the SMCs as intracellular Ca²⁺ waves. The propagation velocity (48 µm/s) of the Ca²⁺ waves was higher than reported by Prakash et al. (2000), who found a velocity of 25 µm/s in isolated porcine tracheal SMCs. Differences in species, airway generation, and/or the different culture form may be responsible. A comparison of the Ca²⁺ oscillations in adjacent SMCs indicated that the Ca²⁺ oscillations occurred asynchronously. If SMCs in slices communicated (e.g., via gap junctions), this did not lead to a synchronization of the Ca²⁺ oscillations. However, all the cells may have been stimulated simultaneously with extracellular ACH, and communication between cells would not be required to initiate a response in each cell. Future experiments with stimulation of single cells will be required to explore the role of cell–cell communication.

Although ACH induced Ca²⁺ oscillations in the absence of extracellular Ca²⁺, these oscillations subsequently ceased. This indicates that an influx of external Ca²⁺ is not directly required for ACH-induced Ca²⁺ signaling, but may be necessary to refill internal Ca²⁺ stores and compensate for Ca²⁺ losses across the cell membrane. Emptying of the internal Ca²⁺ stores using thapsigargin prevented the ACH-induced Ca²⁺ response, confirming the essential role of internal Ca²⁺ release. The abolishment of the Ca²⁺ signaling with the IP₃R antagonist xestospongin indicates that, in murine bronchial SMCs, Ca²⁺ release from the SR occurs in part via the IP₃R. Because the role of Ca²⁺ release via the RYR in agonist-induced Ca²⁺ signaling varies among SMCs of different origins (Savineau and Marthan, 2000), we tested the role of the RYR in murine bronchial SMCs using ryanodine. The ACH-induced Ca²⁺ response was blocked by ryanodine, a result indicating that in these cells Ca²⁺ release also occurs via the RYR. Taken together, we suggest, for the mechanism of ACH-induced Ca²⁺ signaling in murine bronchial SMCs, an initial small IP₃-induced Ca²⁺ release from the SR that is amplified by CICR via RYR with external Ca²⁺ influx occurring to compensate for Ca²⁺ losses to the extracellular space.

Correlation between Ca²⁺ Signaling in SMCs and Airway Contraction
Ca²⁺ contraction coupling in SMCs involves the Ca²⁺–calmodulin complex that activates the myosin-light-chain kinase, which in turn phosphorylates the myosin-light-chain leading to crossbridge cycling and cell contraction. However, Ca²⁺-independent pathways for agonist-induced contraction exist and these may involve diacylglycerol, CPI-17, or Rho-kinases to inhibit myosin-light-chain phosphatase, and thereby increase myosin-light-chain phosphorylation (for reviews see Somlyo and Somlyo, 2000; Pfitzer, 2001). Because we were able to inhibit the ACH-induced airway contraction with intracellular BAPTA, the ACH-induced Ca²⁺ signaling appears to directly mediate contraction in murine bronchial SMCs.

The purpose of the present study was to correlate the Ca²⁺ signaling that occurs in SMCs with airway contraction. Previously, Shieh et al. (1991) observed Ca²⁺ signaling in isolated SMCs and found that an initial Ca²⁺ transient was followed by a lower plateau of [Ca²⁺]_i at a steady state, and suggested that the Ca²⁺ transient establishes tension and that the [Ca²⁺]_i steady state maintains tension. Subsequently, Prakash et al. (2000) found that the steady state of [Ca²⁺]_i, in fact, consists of Ca²⁺ oscillations, and this led them to suggest that Ca²⁺ oscillations may provide a mechanism to regulate or integrate increases in global [Ca²⁺]_i. However, evidence for different contractile states at different frequencies of Ca²⁺ oscillations was not available. In our study, the ACH-induced initial Ca²⁺ transient in the SMCs occurred simultaneously with ACH-induced airway contraction, and this was followed by Ca²⁺ oscillations. It is important to note that these Ca²⁺ oscillations persisted during the maintained airway contraction.

To stop ongoing ACH-induced Ca²⁺ oscillations, we used ACH-esterase to degrade ACH, we inhibited the refilling of the Ca²⁺ stores using Ca²⁺-free solution, or we added halothane to the bath. Pabelick et al. (2001a) could show that halothane reduces SR Ca²⁺ content by increased "leak" through IP₃- and RYR channels. In our study, halothane abolished ACH-induced Ca²⁺ oscillations after a short Ca²⁺ transient. However, in all cases, airway relaxation could be observed upon cessation of the Ca²⁺ oscillations without a substantial change in baseline [Ca²⁺]_i, suggesting an essential role of Ca²⁺ oscillations in the maintenance of airway contraction.

The concentration response curves for ACH-induced Ca²⁺ oscillation frequency and airway contraction were very similar and these data may indicate a close relationship between the extent of airway contraction and the frequency of the Ca²⁺ oscillations. Similarly, in other systems, increases in the frequency of Ca²⁺ oscillations were induced by increasing concentrations of agonists and this led to the idea that frequency-encoding rather than the average [Ca²⁺]_i regulates cellular activities (for reviews see Berridge et al., 2000; Bootman et al., 2001). The hypothesis for the implementation of frequency-modulated control is that the process in question has different activation and inactivation kinetics. For example, the Ca²⁺ oscillation may serve to quickly activate the phosphorylation of the myosin-light-chain via intermediaries while inactivation via dephosphorylation may take longer. As a result, intermittent Ca²⁺ pulses could maintain a prolonged response. In conclusion, we propose that Ca²⁺ oscillations are essential for maintaining the contractile state of the airway SMCs and, thereby, the airway contraction. Further, we hypothesize that this maintenance may be frequency-modulated.

	FOOTNOTES

 
^* Abbreviations used in this paper: ACH, acetylcholine; [Ca²⁺]_i, intracellular Ca²⁺ concentration; DMEM, Dulbecco's modified Eagle's medium; EC, epithelial cell; IP₃, inositol 1,4,5 trisphosphate; IP₃R, IP₃ receptor; MCH, methacholine; ROI, region of interest; sHBSS, supplemented Hanks' balanced salt solution; SMC, smooth muscle cell.

	ACKNOWLEDGMENTS

 
This work was supported by the National Institutes of Health grant HL49288 (to M.J. Sanderson).

Submitted: 16 November 2001
Revised: 9 January 2002
Accepted: 11 January 2002

	REFERENCES

Top ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES

 

Berridge, M.J., P. Lipp, and M.D. Bootman. 2000. The versatility and universality of calcium signalling. Nat. Rev. Mol. Cell Biol. 1:11–21.

Bootman, M.D., T.J. Collins, C.M. Peppiatt, L.S. Prothero, L. MacKenzie, P. De Smet, M. Travers, S.C. Tovey, J.T. Seo, M.J. Berridge, et al. 2001. Calcium signalling: an overview. Semin. Cell Dev. Biol. 12:3–10.

Dandurand, R.J., C.G. Wang, N.C. Phillips, and D.H. Eidelman. 1993. Responsiveness of individual airways to methacholine in adult rat lung explants. J. Appl. Physiol. 75:364–372.

Dandurand, R.J., C.G. Wang, S. Laberge, J.G. Martin, and D.H. Eidelman. 1994. In vitro allergic bronchoconstriction in the brown Norway rat. Am. J. Respir. Crit. Care Med. 149:1499–1505.

Di Virgilio, F., T.H. Steinberg, and S.C. Silverstein. 1990. Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers. Cell Calcium. 11:57–62.

Duguet, A., K. Biyah, E. Minshall, R. Gomes, C.G. Wang, M. Taoudi-Benchekroun, J.H. Bates, and D.H. Eidelman. 2000. Bronchial responsiveness among inbred mouse strains. Role of airway smooth-muscle shortening velocity. Am. J. Respir. Crit. Care Med. 161:839–848.

Hall, I.P. 2000. Second messengers, ion channels and pharmacology of airway smooth muscle. Eur. Respir. J. 15:1120–1127.

Hall, I.P., and M. Kotlikoff. 1995. Use of cultured airway myocytes for study of airway smooth muscle. Am. J. Physiol. 268:L1–L11.

Held, H.D., C. Martin, and S. Uhlig. 1999. Characterization of airway and vascular responses in murine lungs. Br. J. Pharmacol. 126:1191–1199.

Kurosawa, H., C.G. Wang, R.J. Dandurand, M. King, and D.H. Eidelman. 1995. Mucociliary function in the mouse measured in explanted lung tissue. J. Appl. Physiol. 79:41–46.

Liu, X., and J.M. Farley. 1996. Acetylcholine-induced chloride current oscillations in swine tracheal smooth muscle cells. J. Pharmacol. Exp. Ther. 276:178–186.

Martin, C., S. Uhlig, and V. Ullrich. 1996. Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices. Eur. Respir. J. 9:2479–2487.

Martin, C., S. Uhlig, and V. Ullrich. 2001. Cytokine-induced bronchoconstriction in precision-cut lung slices is dependent upon cyclooxygenase-2 and thromboxane receptor activation. Am. J. Respir. Cell Mol. Biol. 24:139–145.

Martin, C., V. Ullrich, and S. Uhlig. 2000a. Effects of the thromboxane receptor agonist U46619 and endothelin-1 on large and small airways. Eur. Respir. J. 16:316–323.

Martin, J.G., A. Duguet, and D.H. Eidelman. 2000b. The contribution of airway smooth muscle to airway narrowing and airway hyperresponsiveness in disease. Eur. Respir. J. 16:349–354.

Minshall, E., C.G. Wang, R. Dandurand, and D. Eidelman. 1997. Heterogeneity of responsiveness of individual airways in cultured lung explants. Can. J. Physiol. Pharmacol. 75:911–916.

Pabelick, C.M., Y.S. Prakash, M.S. Kannan, D.O. Warner, and G.C. Sieck. 2001a. Effects of halothane on sarcoplasmic reticulum calcium release channels in porcine airway smooth muscle cells. Anesthesiology. 95:207–215.

Pabelick, C.M., G.C. Sieck, and Y.S. Prakash. 2001b. Invited review: significance of spatial and temporal heterogeneity of calcium transients in smooth muscle. J. Appl. Physiol. 91:488–496.

Pfitzer, G. 2001. Invited review: regulation of myosin phosphorylation in smooth muscle. J. Appl. Physiol. 91:497–503.

Placke, M.E., and G.L. Fisher. 1987. Adult peripheral lung organ culture: a model for respiratory tract toxicology. Toxicol. Appl. Pharmacol. 90:284–298.

Prakash, Y.S., M.S. Kannan, and G.C. Sieck. 1997. Regulation of intracellular calcium oscillations in porcine tracheal smooth muscle cells. Am. J. Physiol. 272:C966–C975.

Prakash, Y.S., C.M. Pabelick, M.S. Kannan, and G.C. Sieck. 2000. Spatial and temporal aspects of ACh-induced [Ca²⁺]i oscillations in porcine tracheal smooth muscle. Cell Calcium. 27:153–162.

Salerno, F.G., H. Kurosawa, D.H. Eidelman, and M.S. Ludwig. 1996. Characterization of the anatomical structures involved in the contractile response of the rat lung periphery. Br. J. Pharmacol. 118:734–740.

Sanderson, M.J., and I. Parker. 2002. Video-rate confocal microscopy. Methods in Enzymology. G. Marriott and I. Parker, editors. Academic Press, New York. In press.

Savineau, J.P., and R. Marthan. 2000. Cytosolic calcium oscillations in smooth muscle cells. News Physiol. Sci. 15:50–55.

Shieh, C.C., M.F. Petrini, T.M. Dwyer, and J.M. Farley. 1991. Concentration-dependence of acetylcholine-induced changes in calcium and tension in swine trachealis. J. Pharmacol. Exp. Ther. 256:141–148.

Sieck, G.C., M.S. Kannan, and Y.S. Prakash. 1997. Heterogeneity in dynamic regulation of intracellular calcium in airway smooth muscle cells. Can. J. Physiol. Pharmacol. 75:878–888.

Sims, S.M., Y. Jiao, and Z.G. Zheng. 1996. Intracellular calcium stores in isolated tracheal smooth muscle cells. Am. J. Physiol. 271:L300–L309.

Somlyo, A.P., and A.V. Somlyo. 2000. Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J Physiol. 522:177–185.

Tulic, M.K., J.L. Wale, F. Petak, and P.D. Sly. 1999. Muscarinic blockade of methacholine induced airway and parenchymal lung responses in anaesthetised rats. Thorax. 54:531–537.

Widdop, S., K. Daykin, and I.P. Hall. 1993. Expression of muscarinic M2 receptors in cultured human airway smooth muscle cells. Am. J. Respir. Cell Mol. Biol. 9:541–546.

Wohlsen, A., S. Uhlig, and C. Martin. 2001. Immediate allergic response in small airways. Am. J. Respir. Crit. Care Med. 163:1462–1469.

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