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ARTICLE |
Correspondence to Jian Yang: jy160{at}columbia.edu
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1) subunit. Here we investigated the architecture of the inner pore of P/Q-type Ca2+ channels using the substituted-cysteine accessibility method. Many positions in the S6 segments of all four repeats of the 1 subunit (Cav2.1) were modified by internal methanethiosulfonate ethyltrimethylammonium (MTSET). However, the pattern of modification does not fit any known sequence alignment with K+ channels. In IIS6, five consecutive positions showed clear modification, suggesting a likely aqueous crevice and a loose packing between S6 and S5 segments, a notion further supported by the observation that some S5 positions were also accessible to internal MTSET. These results indicate that the inner pore of VGCCs is indeed formed by the S6 segments but is different from that of K+ channels. Interestingly some residues in IIIS6 and IVS6 whose mutations in L-type Ca2+ channels affect the binding of dihydropyridines and phenylalkylamines and are thought to face the pore appeared not to react with internal MTSET. Probing with qBBr, a rigid thiol-reactive agent with a dimension of 12 Å x 10 Å x 6 Å suggests that the inner pore can open to >10 Å. This work provides an impetus for future studies on ion permeation, gating, and drug binding of VGCCs.
Abbreviations used in this paper: BTZ, benzothiazepine; DHP, dihydropyridine; MTSET, methanethiosulfonate ethyltrimethylammonium; PAA, phenylalkylamine; SCAM, substituted-cysteine accessibility method; VGCC, voltage-gated Ca2+ channel.
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INTRODUCTION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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1, ß, 2
, and sometimes 
. The different types of Ca2+ currents are defined by different 1 subunits (Catterall, 2000
). As the primary subunit, 1 is equipped with most functions of the channel: it forms the ion conduction pore, incorporates the voltage sensor and gating apparatus, and carries many sites for regulation by second messengers, G proteins, protein kinases and phosphatases, agonists, antagonists, and toxins. The topology of various 1 subunits is the same, containing four homologous repeats, each consisting of six transmembrane segments (S1–S6) and a pore loop (P-loop) between S5 and S6 (Fig. 1 A). The ß and 2 subunits are auxiliary subunits and regulate channel assembly, trafficking, membrane targeting, activation, and inactivation properties (Catterall, 2000).
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; Tang et al., 1993; Yang et al., 1993; Ellinor et al., 1995). The inner pore of VGCCs is also likely to be important for Ca2+ permeation as well as for gating, as in K+ channels. It also harbors binding sites for organic molecules such as phenylalkylamines (PAAs), benzothiazepines (BTZs), and dihydropyridines (DHPs) (Peterson et al., 1996, 1997; Hockerman et al., 1995, 1997a,b), which are commonly used drugs for the treatment of cardiovascular diseases. In K+ channels, the inner pore is formed by the S6/M2 transmembrane segments from four symmetrically arranged subunits, as revealed by site-directed chemical modifications and x-ray crystallography (Liu et al., 1997; Doyle et al., 1998; Lu et al., 1999a; Jiang et al., 2002a,b, 2003a,b). By contrast, little is known about the architecture of the inner pore of VGCCs, which are thought to be evolved from K+ channels but their pore is formed by four asymmetric domains of the same (1) subunit. There is yet no systematic analysis of which transmembrane regions and amino acids form the inner pore of VGCCs.
In this study, we investigated the architecture of the inner pore of P/Q-type (Cav2.1) Ca2+ channels using the substituted-cysteine accessibility method (SCAM), which has been used in various types of channels to identify pore-lining residues, size the channel, examine the conformational changes in different functional states, and locate the activation gate (e.g., Akabas et al., 1992, 1994; Yang and Horn, 1995; Liu et al., 1997; Lu et al., 1999a,b; Liu and Siegelbaum, 2000; Wu et al., 2000; del Camino and Yellen, 2001; Flynn and Zagotta, 2001; Xiao et al., 2003; for review see Karlin and Akabas, 1998). We systematically mutated residues of all four S6 segments of the 1 subunit (Cav2.1) to cysteines, one at a time, and probed the mutant channel with a water-soluble sulfhydryl-specific reagent, which forms a covalent bond with the sulfhydryl group on the accessible cysteine. Our results indicate that the inner pore of VGCCs shares some properties with that of K+ channels but also possesses certain unique and surprising features. Thus, many positions in the S6 segments of all four repeats were found to be modified by internal methanethiosulfonate ethyltrimethylammonium (MTSET), indicating that the inner pore of VGCCs is indeed formed by S6. However, the pattern of modification does not fit any known sequence alignment with K+ channels and is consistent with an asymmetric architecture. In IIS6, five consecutive positions showed clear modification, suggesting a likely aqueous crevice between S6 and S5 segments, a notion further supported by the observation that some S5 positions were also accessible to internal MTSET. This loose packing of the S6 and S5 segments is different from the tight packing in K+ channels. Finally, some residues in IIIS6 and IVS6 whose mutations in L-type (Cav1.2) channels affect the binding of DHPs and/or PAAs and are thought to face the pore appeared not to react with internal MTSET.
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MATERIALS AND METHODS |
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2 (Mikami et al., 1989) cDNAs are from rabbit brain and skeletal muscle, respectively. ß2a cDNA (M80545) is from rat brain. Site-directed mutations were generated by either PCR mutagenesis or the oligo nucleotide insertion method and were confirmed by sequencing. Eight endogenous cysteines in the N terminus, intracellular loops, IVS6, and C terminus of Cav2.1 were mutated to alanines. The channel formed by this mutant subunit and the 2 and ß subunits is called "the control channel." All cysteine mutations were made on this "control" 1 subunit. All 1 constructs and 2 were cloned in an oocyte expression vector, pGEMHE, or one of its variants. The ß2a subunit was cloned in the Bluescript SK vector; it was chosen because it confers the slowest inactivation, which greatly facilitates our experiments. cRNA for all constructs were transcribed in vitro using the T7 polymerase.
Oocyte Expression
Ovarian lobes were obtained from adult Xenopus laevis under anesthesia. Stage V–VI oocytes were prepared by treatment with 2 mg/ml collagenase A (Roche) for 1.5–2.5 h under 200 rpm shaking in a solution containing 82.4 mM NaCl, 2.5 mM KCl, 1 mM MgCl2, and 5 mM HEPES (pH 7.6) and then rinsed twice (15 min each) with ND96 solution containing 96 mM NaCl, 2.5 mM KCl, 1 mM MgCl2, 5 mM HEPES, 1.8 mM CaCl2, 100 U/ml penicillin, and 100 µg/ml streptomycin (pH 7.6). The 1, 2, and ß2a cRNA were injected into defoliculated oocytes with a total concentration of 
0.5 µg/µl (ratio 1:2:ß2a = 5:5:4) and a volume of 50 nl per oocyte. Injected oocytes were maintained in ND96 solution in an 18°C incubator, and used for recordings 3–6 d after injection.
Electrophysiology
Whole-cell currents recorded with two-electrode voltage-clamp with the OC-725C oocyte clamp amplifier (Warner Instruments) were used mainly for checking the functional expression of mutant channels in oocytes. The recording electrodes were filled with 3 M KCl and had resistances of 0.5–1 M
. The bath solution contained 10 mM BaCl2, 5 mM KCl, 60 mM TEA-OH, 20 mM NaOH, and 5 mM HEPES (pH 7.4 with methanesulphonic acid).
All patch-clamp recordings were performed using the inside-out configuration. Oocyte was bathed in a bath solution containing 125 mM KCl, 4 mM NaCl, 10 mM HEPES, 10 mM EGTA (pH 7.3 with KOH). The control solution, which in addition contained 2 mM Mg-ATP and 3 µM sonicated PIP2, was prepared fresh daily. Recording glass pipettes pulled from pyrex glass tubes (Corning) were filled with a solution of 85 mM BaCl2, 10 mM HEPES (pH 7.3 with KOH), and had a resistance of 0.2–0.3 M. When monovalent current was recorded, the pipettes were filled with the bath solution. Control and test solutions (with the reagent dissolved in the control solution) were fed by gravity through separate tubes to a manifold attached to a single outlet tube and were switched on/off individually. After obtaining the inside-out patches, the recording pipette was inserted into the perfusion tube to achieve a rapid and complete exchange of solution.
Patch-clamp recordings were performed with the Axopatch 200A amplifier (Axon Instruments). Macroscopic currents were evoked from a holding potential of –80 mV every 6 s by a 500-ms depolarization to a voltage that opens >70% of the channels (0 to 40 mV, depending on the channel type). To examine the voltage dependence of activation, currents were evoked from a holding potential of –80 mV every 3 s by 10-ms test potentials from –30 to +100 mV in 10-mV increments, followed by a 50-ms repolarization to –30 mV. Activation curves were constructed by plotting the tail currents at –30 mV against the test potentials. Reversal potential was interpolated from current–voltage curves. All currents were filtered at 2 kHz, digitized at 10 kHz. Data acquisition and analysis were performed using pClamp8 (Axon Instruments) on a PC through a Digidata 1200 interface. Experiments were performed at 21–23°C.
MTS reagents (Toronto Research Chemicals) and qBBr (Molecular Probes) were stored at –20°C and were dissolved in the control solution before each experiment, generally <5 min before application. 4 mM MTS reagents and qBBr were applied for 2–8 min, until modification reached a steady state or the speed of modification could not be distinguished from that of intrinsic current rundown. The effect of a reagent was calculated from the current amplitude obtained before application and after washout of the reagents. Data are represented as mean ± SD (number of observations). Significance was determined using the Mann-Whitney Test. No corrections were made for leakage current, which was negligible at –80 to 100 mV in macropatch recordings.
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RESULTS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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). With such a treatment, the remaining current obtained 10 min after patch excision increased from 34.1% (normalized by the current measured immediately after patch excision) in the bath solution to 85.6% (n = 6). This reduced rundown permitted us to carry out the cysteine modification experiments.
Construction of a Control Channel Nonreactive to Sulfhydryl-specific Modifying Reagents
Another prerequisite for using SCAM is the ability to clearly distinguish the response of the cysteine mutant channels from that of the wild-type channel. The Cav2.1 subunit used in our experiments contains 28 endogenous cysteines, out of which nine are in the extracellular and intracellular loops, respectively, nine in the transmembrane segments, and one in the P-loop. Despite the presence of nine extracellular cysteines, external MTS reagents did not produce significant irreversible current inhibition in the wild-type channel (unpublished data). Similar lack of effect by extracellular sulfhydryl modifiers was also observed in the cardiac L-type Ca2+ channel (Wu et al., 2000). On the other hand, the wild-type P/Q-type channel was irreversibly and completely inhibited by internal MTSEA or MTSET (Fig. 1, B and C), indicating that one or more endogenous cysteines were covalently modified. To eliminate the intrinsic reactivity with internal modifiers, we substituted alanine for eight endogenous cysteines in the NH2 terminus (C71), intracellular loops (C1246, C1378, and C1543), COOH terminus (C1877, C2088, and C2123), and IVS6 (C1807) in the 1 subunit (Fig. 1 A). The channel formed by this mutant 1 subunit with 2 and ß subunits was called "the control channel." It was functional and generated large macroscopic currents in oocytes, the amplitude of which was comparable to that produced by wild-type channels. The activation voltage of the control channel was shifted by –20 mV but the speed of inactivation was little affected (Table I). Importantly, the control channel did not show significant reactivity with internal MTSEA or MTSET (Fig. 1, D and E), and it was also nonreactive to external MTS reagents (not depicted). Furthermore, the activity of the control channel could still be stabilized by Mg-ATP (2 mM) + PIP2 (3 µM) in inside-out patches.
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1 subunit. We aligned the amino acid sequence of all four S6 segments based on a conserved asparagine (Fig. 2 A, top). The putative residue at the interface of the membrane and cytoplasm is defined as position 0. Residues in the membrane are numbered as 1, 2, 3, etc., from inside to outside, and residues beyond the membrane are numbered as –1, –2, –3, etc. To determine putative pore-lining residues, we replaced, one by one, a total of 87 consecutive residues in all four S6 segments with a cysteine, mainly from position 14 to position –3 in each repeat. All but five mutant 1 subunits formed functional channels in oocyte. The five mutants that failed to produce large enough Ba2+ currents are IS6-N8C, IIIS6-Y18C, IIIS6-V20C, IVS6-L7C and IVS6-N8C.
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-helical wheel (Fig. 5), most of the MTSET-reactive positions are located on one side of the S6 helices, supporting the interpretation that they face the lumen of the pore. These results indicate that the S6 segments form the Ca2+ channel inner pore. Notice, however, that the reactive side is broader than the nonreactive side and some reactive positions are located on the back side of the helical wheel. These results will be discussed later.
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To determine whether the functional effect of MTSET on the S6 mutants is due to a change of gating, we examined the voltage dependence of activation before and after MTSET application for four mutant channels, one in each repeat (IS6-L2C, IIS6-N-1C, IIIS6-A4C, and IVS6-V5C). The activation curves of these channels were not significantly altered after modification, as exemplified in Fig. 6. Thus, covalent modification by MTSET most likely inhibits these mutant channels by blocking the ion conduction pathway rather than by changing channel gating. However, MTSET may inhibit currents by different mechanisms at different positions, either by blocking ion conduction or changing gating, or both.
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In IIS5 and IVS5 segments, the first position, which presumably lies at the membrane/cytoplasm interface, was reactive to internal MTSET. More important, position 6 in IIIS5 and positions 3 and 8 in IVS5, which are located deep in the membrane, also showed apparent modification (Fig. 7). The current inhibition caused by MTSET was small, but nevertheless, it was significant. Since MTSET is permanently charged and does not partition into the membrane, modification of these S5 positions suggests that either some portions of S5 contribute to form the inner pore or there is an aqueous crevice between S5 and S6. The latter seems more likely since in K+ channels the S5 segments are clearly behind the pore. If this is indeed the case, the current inhibition caused by MTSET modification is then an allosteric effect rather than a direct block of the pore.
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DISCUSSION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Pore-lining vs. Nonpore-lining Residues
A key assumption in using SCAM to identify pore-lining residues is that the cysteine mutation by itself does not alter the side-chain orientation of the substituted residue and the overall structure of the channel. This assumption is difficult to verify directly, but the fact that the functional properties of many mutant channels were not significantly altered by the cysteine mutation (Table II, Table III, Table IV) suggests that it holds true for those channels. However, many other mutant channels (such as L354C, N707C, I712C, N1513C, and I1514C, to give a few examples) did show significant changes in their activation and/or inactivation properties (Tables I–IV). Some of the changes were probably caused by the highly variable contamination from the Cl– current mediated by the endogenous Ca2+-activated Cl– channels, which can also be activated to a lesser degree by Ba2+, the charge carrier used in our experiments. Other changes could be genuine since the S6 segments are intimately involved in both activation (Xie et al., 2005) and inactivation gating in VGCCs (for review see Hering et al., 2000; Stotz and Zamponi, 2001). For these mutant channels, we can only hope that the aforementioned assumption also holds true. This hope, however, is not a completely blind one since in K+ channels, where many S6/M2 cysteine mutations also greatly altered various channel properties, the putative pore-lining residues identified by SCAM match nicely those revealed directly by x-ray crystallography (Liu et al., 1997; Doyle et al., 1998; Lu et al., 1999a; Kuo et al., 2003), suggesting that the assumption does hold in K+ channels.
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). Although there is a theoretical possibility that modification of a pore-lining residue may be functionally silent, our observation that most of the MTSET-reactive positions form a broad and continuous surface (Fig. 5) makes this possibility seem unlikely.
In this study, accessibility was determined by the change in current amplitude after exposure to MTSET. There are caveats to this approach, particularly for those mutant channels that showed only partial current inhibition (Fig. 3). The partial inhibition itself is not surprising, since only a single cysteine in one of the four pore-lining segments was modified. In K+ channels, modification of a single cysteine in one of the four subunits also produces only 10–25% inhibition (Lu et al., 1999a; Loussouarn et al., 2001). The partial inhibition we observed in this study could not be attributed to channel rundown since we could clearly and easily separate the two processes (e.g., Fig. 4 B). But could it be a nonspecific effect, at least for those mutant channels where an extended period of time (up to 4 min) of MTSET exposure was required to achieve steady-state inhibition? We cannot exclude this possibility, but two reasons argue against it. First, in each S6 segment, most of the reactive positions form a continuous face, bordered by positions 4 and 13 (Fig. 5), rather than dot randomly, as would be expected if modification occurred at random as a result of spontaneous dynamic conformational changes of the channel protein. Second, in our previous work on Kir2.1 channels, we also observed several positions that were modified partially and slowly (Lu et al., 1999a). Those positions correspond to pore-lining positions in the KcsA and MthK crystal structure (Doyle et al., 1998; Jiang et al., 2002b).
Ca2+ Channel Inner Pore Is Asymmetric
The inner pore of K+ channels is formed by the S6/M2 segments from four identical subunits. The inner pore of VGCCs, on the other hand, is formed by the S6 segments from four homologous but nonidentical repeats. Thus, there is an intrinsic amino acid sequence asymmetry to the Ca2+ pore. This asymmetry is also demonstrated by the differential functional impact of MTSET modification. For instance, position 10 was modified in both IIS6 and IVS6, but the resulting current inhibition was dramatically different (85.8 ± 2.6% vs. 31.4 ± 8.8%); furthermore, the same position was not modified in IS6 and IIIS6 (Fig. 3). Another example is that positions 1 and 2 were modified in every repeat; however, the current inhibition was 100% and >70% in IS6, IIS6, and IVS6, but only 52.6 ± 4.1% and 35.1 ± 10.8% in IIIS6 (Fig. 3). These results lend support to the notion that the four S6 segments of VGCCs are functionally nonequivalent, as has been demonstrated by their differential role in the binding of DHPs, PAAs, and BTZs (see below for more discussion). They also suggest that the vertical position and/or orientation of the four S6 segments may be different.
S6 and S5 Transmembrane Segments May Be Loosely Packed
Based on various amino acid sequence alignments, several homology models of the inner pore of VGCCs have been proposed using the crystal structure of KcsA K+ channel as a template (Huber et al., 2000; Zhorov et al., 2001; Lipkind and Fozzard, 2003). The modification pattern of the S6 segments of VGCCs obtained in this study does not fit any of the current homology models and is strikingly different from that of K+ channels (Liu et al., 1997; Lu et al., 1999a). One major difference is that many more positions above the putative membrane/cytoplasm interface are reactive to MTSET in Ca2+ channels than in K+ channels (Fig. 5). Another major difference is that in IIS6, IIIS6, and IVS6 of Ca2+ channels, one or two positions on the opposite or back side of the accessible surface, which most likely forms the lining of the inner pore, are also modified by internal MTSET. This observation suggests that either these S6 segments are not a continuous -helix, which seems unlikely based on secondary structure predictions and the structure of the K+ channel S6/M2 segments, or the pore-lining S6 and surrounding S5 segments are loosely packed, creating an aqueous crevice in between. Consistent with the latter hypothesis, several positions in the S5 segments, some of which are buried deep in the membrane, were found to react with internal MTSET (Fig. 7). The current inhibition caused by modification of these positions is most likely an allosteric effect instead of a direct pore block. It is possible that some other S5 positions were also modified, but their modification was functionally mute.
This type of loose packing between the S5 and S6 transmembrane segments is not present in K+ channels. Our previous study on Kir2.1 channels found that none of the M1 positions were modified by either MTSET or MTSEA, even after long exposure to high concentrations of the modifying reagents (Lu et al., 1999a). More important, the crystal structures of KcsA, MthK, and KvAP all show that M1/S5 is buried in membrane lipids and is positioned immediately behind the pore-forming M2/S6 (Doyle et al., 1998; Jiang et al., 2002a, 2003a). There is clearly no aqueous crevice between M1/S5 and M2/S6 that could accommodate a molecule as big as MTSET.
Ca2+ Channel Inner Pore Is Wide
Our results show that many S6 mutant channels were inhibited by MTSET by varying degrees (Fig. 3). It is possible that for channels that are inhibited partially, the side chain of the modified positions points tangentially to the pore, whereas for those that are inhibited by a relatively large degree (>80%), their side chain points to the pore more or less perpendicularly. Interestingly, several mutant channels are almost completely inhibited, despite the fact that each mutant channel contains only a single modifiable cysteine in a particular repeat. This is strikingly different from what has been observed in K+ channels, where modification of cysteines on all four subunits is required to produce complete inhibition (Lu et al., 1999a; Loussouarn et al., 2001).
To explain this discrepancy between Ca2+ and K+ channels, we first need to understand how MTSET modification impedes ion conduction in Ca2+ channels. Is it by electrostatic repulsion or physical obstruction, or both? To address this question, we examined the effect of MTSEA on IIIS6-V9C, which was almost completely inhibited by MTSET. MTSEA also carries a single positive charge but has a smaller head group (3.6 Å in diameter) than MTSET does. However, it inhibited the current by a similar degree as MTSET did (87.3% and 93.0%, respectively; MTSEA inhibited the control channel by 16.8%) and the modification was faster (unpublished data). This result suggests that electrostatic repulsion between the head group of a modifying reagent and the permeant ions plays a predominant role in blocking the current flow. The much stronger inhibition by MTSET in Ca2+ channels is because the charge carrier is a divalent cation (Ba2+), which naturally leads to stronger electrostatic repulsion. Another piece of evidence supporting this interpretation is the weaker effect of MTSET modification on monovalent currents in Ca2+ channels. To record a monovalent current, we used the same solution on both sides of the membrane patch, with K+ as the charge carrier. The K+ current of IIS6-A4C was inhibited by 86.3% (n = 6), whereas the Ba2+ current was inhibited by 99.2% (n = 4) (Fig. 9). The residual K+ current (14%) was not mediated by endogenous K+ channels, since it was substantially larger than the endogenous K+ currents recorded from oocytes lacking exogenously expressed Ca2+ channels (Fig. 9, compare D and F).
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12 Å (Lu et al., 1999a; Jiang et al., 2002b). Previous work on VGCCs suggests that they may also have a large inner pore. For example, tetramethylammonium, which has a diameter of 6 Å, can pass through the channel (McCleskey and Almers, 1985). Large organic molecules such as PAAs, BTZs, and DHPs are thought to bind within or close to the ion conduction pathway (Hockerman et al., 1997b). Our finding that qBBr can modify residues deep in the inner pore (Fig. 8) suggests that the Ca2+ channel inner pore can open to >10 Å.
Implications for the Binding of DHPs and PAAs
Our results have important implications for understanding the mechanism of the binding and actions of DHPs, PAAs, and BTZs, drugs that are widely used for the treatment of cardiovascular diseases. DHPs act on L-type Ca2+ channel as either agonists favoring the open state or antagonists favoring the inactivated state. Peptide segments contributing to form the DHP-binding site have been localized to IIIS6 and IVS6 of the 1 subunit by photoaffinity labeling and antibody mapping (Nakayama et al., 1991; Striessnig et al., 1991; Kalasz et al., 1993). Site-directed mutagenesis studies have identified single amino acid residues in IIIS6 and IVS6 whose mutations affected DHP binding (Peterson et al., 1996, 1997). These residues are believed to form a high-affinity DHP-binding site. If we align the amino acid sequence of P/Q-type channels with that of L-type channels (Fig. 10), 5 out of the 10 homologous DHP-binding residues were not modified by MTSET (IIIS6: F11C, F14C, and F17C; IVS6: F16C and I17C). Only three of them showed modification (IIIS6-V9C; IVS6: L9C and M10C). The other two cysteine mutant channels (IIIS6-Y18C and IVS6-N8C) did not produce enough Ba2+ current. DHPs are lipophilic compounds that reach their receptor site through the membrane and they are thought to be allosteric modulators (Peterson et al., 1996, 1997; Hockerman et al., 1997b). Our finding that many of the putative DHP-binding residues point away from the pore is in agreement with the idea that DHPs affect channel activity allosterically rather than by blocking the pore directly.
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, 1997a,b), some of which are also involved in DHP binding. It has been hypothesized that PAAs and DHPs bind to different faces of IIIS6 and IVS6 and in some cases to opposite sides of the same amino acid residues (Hockerman et al., 1997b). While DHPs affect channel activity allosterically, PAAs block the pore directly (Hockerman et al., 1995, 1997a,b). However, among the seven analogous putative PAA-binding residues in P/Q-type channels, only three (IIIS6-V5C; IVS6: M10C and S13C) were modified by MTSET (Fig. 10). Except for IIIS6-Y18C that did not produce enough Ba2+ current, the other three residues were not modified and are therefore unlikely to line the pore. These results suggest that some of the residues whose mutations affect PAA block may not participate directly in PAA binding or part of PAA may interact with hydrophobic residues pointing away from the pore.
Several models have been proposed for the binding of DHPs and PAAs to Ca2+ channels (Huber et al., 2000; Zhorov et al., 2001; Lipkind and Fozzard, 2003). They are all based on the KcsA structure and therefore need further improvement. Our results provide data for constructing a more realistic model. In particular, the proposed loose packing between the S5 and S6 transmembrane segments in VGCCs should be a key consideration in future modeling. This loose packing may confer certain flexibility to the S5 and S6 segments that would permit the creation of a binding pocket suitable for bulky molecules such as DHPs, PAAs, and BTZs.
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ACKNOWLEDGMENTS |
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2 cDNA. This work was supported by National Institutes of Health grant NS45383.
Lawrence G. Palmer served as editor.
Submitted: 28 March 2005
Accepted: 2 August 2005
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REFERENCES |
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