The Journal of General Physiology, Vol 100, 573-591, Copyright © 1992 by The Rockefeller University Press

ARTICLES

Effect of ATP-sensitive K+ channel regulators on cystic fibrosis transmembrane conductance regulator chloride currents

DN Sheppard and MJ Welsh
Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.

The cystic fibrosis transmembrane conductance regulator (CFTR) is a Cl- channel that is regulated by cAMP-dependent phosphorylation and by intracellular ATP. Intracellular ATP also regulates a class of K+ channels that have a distinct pharmacology: they are inhibited by sulfonylureas and activated by a novel class of drugs called K+ channel openers. In search of modulators of CFTR Cl- channels, we examined the effect of sulfonylureas and K+ channel openers on CFTR Cl- currents in cells expressing recombinant CFTR. The sulfonylureas, tolbutamide and glibenclamide, inhibited whole-cell CFTR Cl- currents at half-maximal concentrations of approximately 150 and 20 microM, respectively. Inhibition by both agents showed little voltage dependence and developed slowly; > 90% inhibition occurred 3 min after adding 1 mM tolbutamide or 100 microM glibenclamide. The effect of tolbutamide was reversible, while that of glibenclamide was not. In contrast to their activating effect on K+ channels, the K+ channel openers, diazoxide, BRL 38227, and minoxidil sulfate inhibited CFTR Cl- currents. Half- maximal inhibition was observed at approximately 250 microM diazoxide, 50 microM BRL 38227, and 40 microM minoxidil sulfate. The rank order of potency for inhibition of CFTR Cl- currents was: glibenclamide < BRL 38227 approximately equal to minoxidil sulfate > tolbutamide > diazoxide. Site-directed mutations of CFTR in the first membrane- spanning domain and second nucleotide-binding domain did not affect glibenclamide inhibition of CFTR Cl- currents. However, when part of the R domain was deleted, glibenclamide inhibition showed significant voltage dependence. These agents, especially glibenclamide, which was the most potent, may be of value in identifying CFTR Cl- channels. They or related analogues might also prove to be of value in treating diseases such as diarrhea, which may involve increased activity of the CFTR Cl- channel.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				B. M. Rollins, M. Burn, R. D. Coakley, L. A. Chambers, A. J. Hirsh, M. T. Clunes, M. I. Lethem, S. H. Donaldson, and R. Tarran A2B Adenosine Receptors Regulate the Mucus Clearance Component of the Lung's Innate Defense System Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 190 - 197. [Abstract] [Full Text] [PDF]

				C. Chassin, M. W. Hornef, M. Bens, M. Lotz, J.-M. Goujon, S. Vimont, G. Arlet, A. Hertig, E. Rondeau, and A. Vandewalle Hormonal control of the renal immune response and antibacterial host defense by arginine vasopressin J. Exp. Med., November 26, 2007; 204(12): 2837 - 2852. [Abstract] [Full Text] [PDF]

				C. Routaboul, C. Norez, P. Melin, M.-C. Molina, B. Boucherle, F. Bossard, S. Noel, R. Robert, C. Gauthier, F. Becq, et al. Discovery of {alpha}-Aminoazaheterocycle-Methylglyoxal Adducts as a New Class of High-Affinity Inhibitors of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channels J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 1023 - 1035. [Abstract] [Full Text] [PDF]

				S. M. Sidani, P. Kirchhoff, T. Socrates, L. Stelter, E. Ferreira, C. Caputo, K. E. Roberts, R. L. Bell, M. E. Egan, and J. P. Geibel {Delta}F508 Mutation Results in Impaired Gastric Acid Secretion J. Biol. Chem., March 2, 2007; 282(9): 6068 - 6074. [Abstract] [Full Text] [PDF]

				S. J. Suh, Y.-F. Wang, A. Frelet, N. Leonhardt, M. Klein, C. Forestier, B. Mueller-Roeber, M. H. Cho, E. Martinoia, and J. I. Schroeder The ATP Binding Cassette Transporter AtMRP5 Modulates Anion and Calcium Channel Activities in Arabidopsis Guard Cells J. Biol. Chem., January 19, 2007; 282(3): 1916 - 1924. [Abstract] [Full Text] [PDF]

				R. A. Dombkowski, M. M. Doellman, S. K. Head, and K. R. Olson Hydrogen sulfide mediates hypoxia-induced relaxation of trout urinary bladder smooth muscle J. Exp. Biol., August 15, 2006; 209(16): 3234 - 3240. [Abstract] [Full Text] [PDF]

				R. Robert, C. Norez, and F. Becq Disruption of CFTR chloride channel alters mechanical properties and cAMP-dependent Cl- transport of mouse aortic smooth muscle cells J. Physiol., October 15, 2005; 568(2): 483 - 495. [Abstract] [Full Text] [PDF]

				M. Schuier, H. Sies, B. Illek, and H. Fischer Cocoa-Related Flavonoids Inhibit CFTR-Mediated Chloride Transport across T84 Human Colon Epithelia J. Nutr., October 1, 2005; 135(10): 2320 - 2325. [Abstract] [Full Text] [PDF]

				C. Deachapunya, S. Poonyachoti, W. Thongsaard, and N. Krishnamra Barakol Extracted from Cassia siamea Stimulates Chloride Secretion in Rat Colon J. Pharmacol. Exp. Ther., August 1, 2005; 314(2): 732 - 737. [Abstract] [Full Text] [PDF]

				G. Liang, A. H. Stephenson, A. J. Lonigro, and R. S. Sprague Erythrocytes of humans with cystic fibrosis fail to stimulate nitric oxide synthesis in isolated rabbit lungs Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1580 - H1585. [Abstract] [Full Text] [PDF]

				B. Chen, G. Nicol, and W. K. Cho Electrophysiological characterization of volume-activated chloride currents in mouse cholangiocyte cell line Am J Physiol Gastrointest Liver Physiol, December 1, 2004; 287(6): G1158 - G1167. [Abstract] [Full Text] [PDF]

				M. E. Krouse, J. F. Talbott, M. M. Lee, N. S. Joo, and J. J. Wine Acid and base secretion in the Calu-3 model of human serous cells Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1274 - L1283. [Abstract] [Full Text] [PDF]

				T. Irokawa, M. E. Krouse, N. S. Joo, J. V. Wu, and J. J. Wine A "virtual gland" method for quantifying epithelial fluid secretion Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L784 - L793. [Abstract] [Full Text] [PDF]

				G. G.-L. Yue, T. W.-N. Yip, Y. Huang, and W.-H. Ko Cellular Mechanism for Potentiation of Ca2+-mediated Cl- Secretion by the Flavonoid Baicalein in Intestinal Epithelia J. Biol. Chem., September 17, 2004; 279(38): 39310 - 39316. [Abstract] [Full Text] [PDF]

				H. Chen, L. L. Liu, L. L. Ye, C. McGuckin, S. Tamowski, P. Scowen, H. Tian, K. Murray, W. J. Hatton, and D. Duan Targeted Inactivation of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel Gene Prevents Ischemic Preconditioning in Isolated Mouse Heart Circulation, August 10, 2004; 110(6): 700 - 704. [Abstract] [Full Text] [PDF]

				T. J. F. Nieland, A. Chroni, M. L. Fitzgerald, Z. Maliga, V. I. Zannis, T. Kirchhausen, and M. Krieger Cross-inhibition of SR-BI- and ABCA1-mediated cholesterol transport by the small molecules BLT-4 and glyburide J. Lipid Res., July 1, 2004; 45(7): 1256 - 1265. [Abstract] [Full Text] [PDF]

				A. Currid, B. Ortega, and M. A. Valverde Chloride secretion in a morphologically differentiated human colonic cell line that expresses the epithelial Na+ channel J. Physiol., February 15, 2004; 555(1): 241 - 250. [Abstract] [Full Text] [PDF]

				M. Chen, Y. Dong, and J. M. Simard Functional Coupling between Sulfonylurea Receptor Type 1 and a Nonselective Cation Channel in Reactive Astrocytes from Adult Rat Brain J. Neurosci., September 17, 2003; 23(24): 8568 - 8577. [Abstract] [Full Text] [PDF]

				G. Laverty, C. McWilliams, A. Sheldon, and S. S. Arnason PTH stimulates a Cl--dependent and EIPA-sensitive current in chick proximal tubule cells in culture Am J Physiol Renal Physiol, May 1, 2003; 284(5): F987 - F995. [Abstract] [Full Text] [PDF]

				U. I. M. Wiehart, G. Klein, P. Steels, S. W. Nicolson, and E. Van Kerkhove K+ transport in Malpighian tubules of Tenebrio molitor L.: is a KATP channel involved? J. Exp. Biol., March 15, 2003; 206(6): 959 - 965. [Abstract] [Full Text] [PDF]

				J. Jiang, Y. Song, C. Bai, B. H. Koller, M. A. Matthay, and A. S. Verkman Pleural surface fluorescence measurement of Na+ and Cl- transport across the air space-capillary barrier J Appl Physiol, January 1, 2003; 94(1): 343 - 352. [Abstract] [Full Text] [PDF]

				W. K. Cho Characterization of regulatory volume decrease in freshly isolated mouse cholangiocytes Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1320 - G1327. [Abstract] [Full Text] [PDF]

				L. P. Sullivan, D. P. Wallace, T. Gover, P. A. Welling, T. Yamaguchi, R. Maser, J. W. Eppler, and J. J. Grantham Sulfonylurea-Sensitive K+ Transport is Involved in Cl- Secretion and Cyst Growth by Cultured ADPKD Cells J. Am. Soc. Nephrol., November 1, 2002; 13(11): 2619 - 2627. [Abstract] [Full Text] [PDF]

				Z. Zhou, S. Hu, and T.-C. Hwang Probing an Open CFTR Pore with Organic Anion Blockers J. Gen. Physiol., October 29, 2002; 120(5): 647 - 662. [Abstract] [Full Text] [PDF]

				D. Wendehenne, O. Lamotte, J.-M. Frachisse, H. Barbier-Brygoo, and A. Pugin Nitrate Efflux Is an Essential Component of the Cryptogein Signaling Pathway Leading to Defense Responses and Hypersensitive Cell Death in Tobacco PLANT CELL, August 1, 2002; 14(8): 1937 - 1951. [Abstract] [Full Text] [PDF]

				H. Kulaksiz, A. Schmid, M. Honscheid, A. Ramaswamy, and Y. Cetin Clara cell impact in air-side activation of CFTR in small pulmonary airways PNAS, May 14, 2002; 99(10): 6796 - 6801. [Abstract] [Full Text] [PDF]

				T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]

				H Ishiguro, S Naruse, M Kitagawa, T Mabuchi, T Kondo, T Hayakawa, R M Case, and M C Steward Chloride transport in microperfused interlobular ducts isolated from guinea-pig pancreas J. Physiol., February 15, 2002; 539(1): 175 - 189. [Abstract] [Full Text] [PDF]

				G. E. Knight, P. Bodin, W. C. De Groat, and G. Burnstock ATP is released from guinea pig ureter epithelium on distension Am J Physiol Renal Physiol, February 1, 2002; 282(2): F281 - F288. [Abstract] [Full Text] [PDF]

				M. Kulka, M. Gilchrist, M. Duszyk, and A. D. Befus Expression and functional characterization of CFTR in mast cells J. Leukoc. Biol., January 1, 2002; 71(1): 54 - 64. [Abstract] [Full Text] [PDF]

				D. G. Perregaux, P. McNiff, R. Laliberte, N. Hawryluk, H. Peurano, E. Stam, J. Eggler, R. Griffiths, M. A. Dombroski, and C. A. Gabel Identification and Characterization of a Novel Class of Interleukin-1 Post-Translational Processing Inhibitors J. Pharmacol. Exp. Ther., October 1, 2001; 299(1): 187 - 197. [Abstract] [Full Text] [PDF]

				A. Moran, J. Phillips, and C. Milla Insulin and Glucose Excursion Following Premeal Insulin Lispro or Repaglinide in Cystic Fibrosis-Related Diabetes Diabetes Care, October 1, 2001; 24(10): 1706 - 1710. [Abstract] [Full Text] [PDF]

				R. Toriano, A. Kierbel, M. A. Ramirez, G. Malnic, and M. Parisi Spontaneous water secretion in T84 cells: effects of STa enterotoxin, bumetanide, VIP, forskolin, and A-23187 Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G816 - G822. [Abstract] [Full Text] [PDF]

				M. Bens, J.-P. D. Van Huyen, F. Cluzeaud, J. Teulon, and A. Vandewalle CFTR disruption impairs cAMP-dependent Cl- secretion in primary cultures of mouse cortical collecting ducts Am J Physiol Renal Physiol, September 1, 2001; 281(3): F434 - F442. [Abstract] [Full Text] [PDF]

				D. P. Wallace, L. A. Rome, L. P. Sullivan, and J. J. Grantham cAMP-dependent fluid secretion in rat inner medullary collecting ducts Am J Physiol Renal Physiol, June 1, 2001; 280(6): F1019 - F1029. [Abstract] [Full Text] [PDF]

				Z. Zhou, S. Hu, and T.-C. Hwang Voltage-dependent flickery block of an open cystic fibrosis transmembrane conductance regulator (CFTR) channel pore J. Physiol., April 15, 2001; 532(2): 435 - 448. [Abstract] [Full Text] [PDF]

				K. Kirk Membrane Transport in the Malaria-Infected Erythrocyte Physiol Rev, April 1, 2001; 81(2): 495 - 537. [Abstract] [Full Text] [PDF]

				J.-P. Duong Van Huyen, M. Bens, J. Teulon, and A. Vandewalle Vasopressin-stimulated chloride transport in transimmortalized mouse cell lines derived from the distal convoluted tubule and cortical and inner medullary collecting ducts Nephrol. Dial. Transplant., February 1, 2001; 16(2): 238 - 245. [Abstract] [Full Text] [PDF]

				B. Illek, M. E. Lizarzaburu, V. Lee, M. H. Nantz, M. J. Kurth, and H. Fischer Structural determinants for activation and block of CFTR-mediated chloride currents by apigenin Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1838 - C1846. [Abstract] [Full Text] [PDF]

				E. K. O'Donnell, R. L. Sedlacek, A. K. Singh, and B. D. Schultz Inhibition of enterotoxin-induced porcine colonic secretion by diarylsulfonylureas in vitro Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G1104 - G1112. [Abstract] [Full Text] [PDF]

				A Boom, P E Golstein, M Frerotte, J V. Sande, and R Beauwens Inhibition of basolateral cAMP permeability in the toad urinary bladder J. Physiol., October 1, 2000; 528(1): 189 - 198. [Abstract] [Full Text] [PDF]

				M. Morishita, Y. Iwasaki, E. Yamamori, A. Nomura, N. Mutsuga, M. Yoshida, M. Asai, Y. Oiso, and H. Saito Antidiabetic Sulfonylurea Enhances Secretagogue-Induced Adrenocorticotropin Secretion and Proopiomelanocortin Gene Expression in Vitro Endocrinology, September 1, 2000; 141(9): 3313 - 3318. [Abstract] [Full Text] [PDF]

				C. A. Goddard, M. J. Evans, and W. H. Colledge Genistein activates CFTR-mediated Cl- secretion in the murine trachea and colon Am J Physiol Cell Physiol, August 1, 2000; 279(2): C383 - C392. [Abstract] [Full Text] [PDF]

				K. HANAOKA and W. B. GUGGINO cAMP Regulates Cell Proliferation and Cyst Formation in Autosomal Polycystic Kidney Disease Cells J. Am. Soc. Nephrol., July 1, 2000; 11(7): 1179 - 1187. [Abstract] [Full Text]

				W.-K. Chan, X. Yao, W.-H. Ko, and Y. Huang Nitric oxide mediated endothelium-dependent relaxation induced by glibenclamide in rat isolated aorta Cardiovasc Res, April 1, 2000; 46(1): 180 - 187. [Abstract] [Full Text] [PDF]

				A. Hazama, H.-T. Fan, I. Abdullaev, E. Maeno, S. Tanaka, Y. Ando-Akatsuka, and Y. Okada Swelling-activated, cystic fibrosis transmembrane conductance regulator-augmented ATP release and Cl- conductances in murine C127 cells J. Physiol., February 15, 2000; 523(1): 1 - 11. [Abstract] [Full Text] [PDF]

				A. K. Singh, D. C. Devor, A. C. Gerlach, M. Gondor, J. M. Pilewski, and R. J. Bridges Stimulation of Cl- Secretion by Chlorzoxazone J. Pharmacol. Exp. Ther., February 1, 2000; 292(2): 778 - 787. [Abstract] [Full Text]

				A. S. Lader, Y. Wang, G. R. Jackson Jr., S. C. Borkan, and H. F. Cantiello cAMP-activated anion conductance is associated with expression of CFTR in neonatal mouse cardiac myocytes Am J Physiol Cell Physiol, February 1, 2000; 278(2): C436 - C450. [Abstract] [Full Text] [PDF]

				N. McCarty Permeation through the CFTR chloride channel J. Exp. Biol., January 7, 2000; 203(13): 1947 - 1962. [Abstract] [PDF]

				J. H. Widdicombe Yet Another Role for the Cystic Fibrosis Transmembrane Conductance Regulator Am. J. Respir. Cell Mol. Biol., January 1, 2000; 22(1): 11 - 14. [Full Text]

				J. R. Hume, D. Duan, M. L. Collier, J. Yamazaki, and B. Horowitz Anion Transport in Heart Physiol Rev, January 1, 2000; 80(1): 31 - 81. [Abstract] [Full Text] [PDF]

				S. W. Schneider, M. E. Egan, B. P. Jena, W. B. Guggino, H. Oberleithner, and J. P. Geibel Continuous detection of extracellular ATP on living cells by using atomic force microscopy PNAS, October 12, 1999; 96(21): 12180 - 12185. [Abstract] [Full Text] [PDF]

				S. T. Ballard, L. Trout, Z. Bebok, E. J. Sorscher, and A. Crews CFTR involvement in chloride, bicarbonate, and liquid secretion by airway submucosal glands Am J Physiol Lung Cell Mol Physiol, October 1, 1999; 277(4): L694 - L699. [Abstract] [Full Text] [PDF]

				F. Becq, Y. Mettey, M. A. Gray, L. J. V. Galietta, R. L. Dormer, M. Merten, T. Metaye, V. Chappe, C. Marvingt-Mounir, O. Zegarra-Moran, et al. Development of Substituted Benzo[c]quinolizinium Compounds as Novel Activators of the Cystic Fibrosis Chloride Channel J. Biol. Chem., September 24, 1999; 274(39): 27415 - 27425. [Abstract] [Full Text] [PDF]

				N. Leonhardt, A. Vavasseur, and C. Forestier ATP Binding Cassette Modulators Control Abscisic Acid–Regulated Slow Anion Channels in Guard Cells PLANT CELL, June 1, 1999; 11(6): 1141 - 1152. [Abstract] [Full Text]

				W. ZHANG, I. MANNAN, S. SCHULZ, S. J. PARKINSON, A. E. ALEKSEEV, L. A. GOMEZ, A. TERZIC, and S. A. WALDMAN Interruption of transmembrane signaling as a novel antisecretory strategy to treat enterotoxigenic diarrhea FASEB J, May 1, 1999; 13(8): 913 - 922. [Abstract] [Full Text]

				S. Sorota Insights into the structure, distribution and function of the cardiac chloride channels Cardiovasc Res, May 1, 1999; 42(2): 361 - 376. [Abstract] [Full Text] [PDF]

				D. N. SHEPPARD and M. J. WELSH Structure and Function of the CFTR Chloride Channel Physiol Rev, January 1, 1999; 79(1): 23 - 45. [Abstract] [Full Text] [PDF]

				D. C. DAWSON, S. S. SMITH, and M. K. MANSOURA CFTR: Mechanism of Anion Conduction Physiol Rev, January 1, 1999; 79(1): 47 - 75. [Abstract] [Full Text] [PDF]

				B. D. SCHULTZ, A. K. SINGH, D. C. DEVOR, and R. J. BRIDGES Pharmacology of CFTR Chloride Channel Activity Physiol Rev, January 1, 1999; 79(1): 109 - 144. [Abstract] [Full Text] [PDF]

				E. M. SCHWIEBERT, D. J. BENOS, M. E. EGAN, M. J. STUTTS, and W. B. GUGGINO CFTR Is a Conductance Regulator as well as a Chloride Channel Physiol Rev, January 1, 1999; 79(1): 145 - 166. [Abstract] [Full Text] [PDF]

				M. M. Reddy and P. M. Quinton Bumetanide blocks CFTR GCl in the native sweat duct Am J Physiol Cell Physiol, January 1, 1999; 276(1): C231 - C237. [Abstract] [Full Text] [PDF]

				Y. Liu, S. Oiki, T. Tsumura, T. Shimizu, and Y. Okada Glibenclamide blocks volume-sensitive Cl- channels by dual mechanisms Am J Physiol Cell Physiol, August 1, 1998; 275(2): C343 - C351. [Abstract] [Full Text] [PDF]

				A. Ruknudin, D. H. Schulze, S. K. Sullivan, W. J. Lederer, and P. A. Welling Novel Subunit Composition of a Renal Epithelial KATP Channel J. Biol. Chem., June 5, 1998; 273(23): 14165 - 14171. [Abstract] [Full Text] [PDF]

				J. L. Bankers-Fulbright, G. M. Kephart, D. A. Loegering, A. L. Bradford, S. Okada, H. Kita, and G. J. Gleich Sulfonylureas Inhibit Cytokine-Induced Eosinophil Survival and Activation J. Immunol., June 1, 1998; 160(11): 5546 - 5553. [Abstract] [Full Text] [PDF]

				P. Linsdell and J. W. Hanrahan Adenosine Triphosphate-dependent Asymmetry of Anion Permeation in the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel J. Gen. Physiol., April 1, 1998; 111(4): 601 - 614. [Abstract] [Full Text] [PDF]

				A. Ishida-Takahashi, H. Otani, C. Takahashi, T. Washizuka, K. Tsuji, M. Noda, M. Horie, and S. Sasayama Cystic fibrosis transmembrane conductance regulator mediates sulphonylurea block of the inwardly rectifying K+ channel Kir6.1 J. Physiol., April 1, 1998; 508(1): 23 - 30. [Abstract] [Full Text] [PDF]

				K. B. Walsh and C. Wang Arylaminobenzoate Block of the Cardiac Cyclic AMP-Dependent Chloride Current Mol. Pharmacol., March 1, 1998; 53(3): 539 - 546. [Abstract] [Full Text]

				R. G. Morris, A. Tousson, D. J. Benos, and J. A. Schafer Microtubule disruption inhibits AVT-stimulated Cl- secretion but not Na+ reabsorption in A6 cells Am J Physiol Renal Physiol, February 1, 1998; 274(2): F300 - F314. [Abstract] [Full Text] [PDF]

				D. C. Devor and R. A. Frizzell Modulation of K+ channels by arachidonic acid in T84 cells. I. Inhibition of the Ca2+-dependent K+ channel Am J Physiol Cell Physiol, January 1, 1998; 274(1): C138 - C148. [Abstract] [Full Text] [PDF]

				N. Leonhardt, E. Marin, A. Vavasseur, and C. Forestier Evidence for the existence of a sulfonylurea-receptor-like protein in plants: Modulation of stomatal movements and guard cell potassium channels by sulfonylureas and potassium channel openers PNAS, December 9, 1997; 94(25): 14156 - 14161. [Abstract] [Full Text] [PDF]

				B. D. Schultz, A. Takahashi, C. Liu, R. A. Frizzell, and M. Howard FLAG epitope positioned in an external loop preserves normal biophysical properties of CFTR Am J Physiol Cell Physiol, December 1, 1997; 273(6): C2080 - C2089. [Abstract] [Full Text] [PDF]

				D. C. Dawson and S. S. Smith Commentary Cystic Fibrosis Transmembrane Conductance Regulator: Permeant Ions Find the Pore J. Gen. Physiol., October 1, 1997; 110(4): 337 - 339. [Full Text] [PDF]

				J. Yamazaki and J. R. Hume Inhibitory Effects of Glibenclamide on Cystic Fibrosis Transmembrane Regulator, Swelling-Activated, and Ca2+-Activated Cl- Channels in Mammalian Cardiac Myocytes Circ. Res., July 19, 1997; 81(1): 101 - 109. [Abstract] [Full Text]

				E. A. Pasyk and J. K. Foskett Cystic Fibrosis Transmembrane Conductance Regulator-associated ATP and Adenosine 3'-Phosphate 5'-Phosphosulfate Channels in Endoplasmic Reticulum and Plasma Membranes J. Biol. Chem., March 21, 1997; 272(12): 7746 - 7751. [Abstract] [Full Text] [PDF]

				A. B. Vaandrager, B. C. Tilly, A. Smolenski, S. Schneider-Rasp, A. G.M. Bot, M. Edixhoven, B. J. Scholte, T. Jarchau, U. Walter, S. M. Lohmann, et al. cGMP Stimulation of Cystic Fibrosis Transmembrane Conductance Regulator Cl- Channels Co-expressed with cGMP-dependent Protein Kinase Type II but Not Type Ibeta J. Biol. Chem., February 14, 1997; 272(7): 4195 - 4200. [Abstract] [Full Text] [PDF]

				F. Becq, Y. Hamon, A. Bajetto, M. Gola, B. Verrier, and G. Chimini ABC1, an ATP Binding Cassette Transporter Required for Phagocytosis of Apoptotic Cells, Generates a Regulated Anion Flux after Expression in Xenopus laevis Oocytes J. Biol. Chem., January 31, 1997; 272(5): 2695 - 2699. [Abstract] [Full Text] [PDF]

				S. J. Parkinson, A. E. Alekseev, L. A. Gomez, F. Wagner, A. Terzic, and S. A. Waldman Interruption of Escherichia coli Heat-stable Enterotoxin-induced Guanylyl Cyclase Signaling and Associated Chloride Current in Human Intestinal Cells by 2-Chloroadenosine J. Biol. Chem., January 10, 1997; 272(2): 754 - 758. [Abstract] [Full Text] [PDF]

				A. F. James, T. Tominaga, Y. Okada, and M. Tominaga Distribution of cAMP-Activated Chloride Current and CFTR mRNA in the Guinea Pig Heart Circ. Res., August 1, 1996; 79(2): 201 - 207. [Abstract] [Full Text]

				M. Tominaga, T. Tominaga, A. Miwa, and Y. Okada Volume-sensitive Chloride Channel Activity Does Not Depend on Endogenous P-glycoprotein J. Biol. Chem., November 17, 1995; 270(46): 27887 - 27893. [Abstract] [Full Text] [PDF]

				Q al-Awqati Regulation of ion channels by ABC transporters that secrete ATP Science, August 11, 1995; 269(5225): 805 - 806. [PDF]

				M. Tominaga, M. Horie, S. Sasayama, and Y. Okada Glibenclamide, an ATP-Sensitive K+ Channel Blocker, Inhibits Cardiac cAMP-Activated Cl- Conductance Circ. Res., August 1, 1995; 77(2): 417 - 423. [Abstract] [Full Text]

				S. E. Gabriel, M. Makhlina, E. Martsen, E. J. Thomas, M. I. Lethem, and R. C. Boucher Permeabilization via the P2X7 Purinoreceptor Reveals the Presence of a Ca2+-activated Cl- Conductance in the Apical Membrane of Murine Tracheal Epithelial Cells J. Biol. Chem., November 3, 2000; 275(45): 35028 - 35033. [Abstract] [Full Text] [PDF]

				N. Wang, D. L. Silver, C. Thiele, and A. R. Tall ATP-binding Cassette Transporter A1 (ABCA1) Functions as a Cholesterol Efflux Regulatory Protein J. Biol. Chem., June 22, 2001; 276(26): 23742 - 23747. [Abstract] [Full Text] [PDF]

				W. H. Ko, V. W. Y. Law, W. C. Y. Yip, G. G. L. Yue, C. W. Lau, Z. Y. Chen, and Y. Huang Stimulation of chloride secretion by baicalein in isolated rat distal colon Am J Physiol Gastrointest Liver Physiol, March 1, 2002; 282(3): G508 - G518. [Abstract] [Full Text] [PDF]

				A. Lazrak, U. Thome, C. Myles, J. Ware, L. Chen, C. J. Venglarik, and S. Matalon Alveolar Epithelial Ion and Fluid Transport: cAMP regulation of Cl- and HCO3- secretion across rat fetal distal lung epithelial cells Am J Physiol Lung Cell Mol Physiol, April 1, 2002; 282(4): L650 - L658. [Abstract] [Full Text] [PDF]

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents