July 2014 | Volume 144, No. 1
- A nonequilibrium binary elements-based kinetic model for benzodiazepine regulation of GABAA receptors
A nonequilibrium kinetic model that explicitly treats the energetics of interactions between structural domains is used to describe positive modulation of the GABAA receptor by benzodiazepines.
- Determinants of ligand selectivity in a cyclic nucleotide–regulated potassium channel
Preference for cGMP binding to a cyclic nucleotide–binding domain can achieved by compensating for ligand dehydration or through retention of solvation waters in the bound state.
- Conductance and block of hair-cell mechanotransducer channels in transmembrane channel–like protein mutants
Proteins other than TMC1 and TMC2 must contribute to the pore of the mechanotransducer channel of cochlear hair cells; an external vestibule subject to disruption in Tmc mutants may influence the channel’s properties.
- Concerted loop motion triggers induced fit of FepA to ferric enterobactin
The loops of the bacterial outer membrane iron transporter FepA move at different rates to adsorb and grasp the substrate ferric enterobactin before transporting it into the periplasm.
Methods and Approaches
- Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin
A P2X4 receptor labeled with the pH-sensitive GFP superecliptic pHluorin represents a useful probe to investigate P2X4 receptor distribution, trafficking, and up-regulation.
- Calmodulation meta-analysis: Predicting calmodulin binding via canonical motif clustering
Computational analysis of target sequences from calmodulin–peptide structures indicates that calmodulin often binds to sequences with multiple overlapping canonical calmodulin-binding motifs.