Kv7.1 voltage-gated K+ (Kv) channels are present in the apical membranes of marginal cells of the stria vascularis of the inner ear, where they mediate K+ efflux into the scala media (cochlear duct) of the cochlea. As such, they are exposed to the K+-rich (∼150 mM of external K+ (K+e)) environment of the endolymph. Previous studies have shown that Kv7.1 currents are substantially suppressed by high K+e (independent of the effects of altering the electrochemical gradient). However, the molecular basis for this inhibition, which is believed to involve stabilization of an inactivated state, remains unclear. Using sequence alignment of S5-pore linkers of several Kv channels, we identified a key residue, E290, found in only a few Kv channels including Kv7.1. We used substituted cysteine accessibility methods and patch-clamp analysis to provide evidence that the ability of Kv7.1 to sense K+e depends on E290, and that the charge at this position is essential for Kv7.1’s K+e sensitivity. We propose that Kv7.1 may use this feedback mechanism to maintain the magnitude of the endocochlear potential, which boosts the driving force to generate the receptor potential of hair cells. The implications of our findings transcend the auditory system; mutations at this position also result in long QT syndrome in the heart.
Kv7.1 (KCNQ1 or KvLQT1) voltage-gated K+ (Kv) channel is encoded by the KCNQ1 gene. Mutations in the KCNQ1 gene have been shown to lead to long QT syndrome (LQTS), a hereditary condition associated with life-threatening cardiac arrhythmias (Wang et al., 1996; Splawski et al., 2000; Tester et al., 2005). Kv7.1 channels also play critical roles in the inner ear, where they mediate K+ flux across the apical membrane of the marginal cells (MCs) of the stria vascularis into the scala media of the cochlear duct (Wangemann, 2002). The flux of K+ across cells of the stria vascularis and the high input impedance of a 15-nm interstrial space establish the endocochlear potential, which enhances hair-cell generator potential by approximately twofold (Salt et al., 1987; Nin et al., 2008). Kv7.1 has been shown to be prominently expressed in MCs of the stria vascularis (Wangemann, 2002). Null or missense mutations of Kv7.1 result in hearing loss (Schmitt et al., 2000; Casimiro et al., 2004; Maljevic et al., 2008). In the cochlear duct, Kv7.1 is exposed to ∼150 mM of external K+ (K+e), imposing an unfavorable chemical gradient for the flux of K+ from MCs into the scala media (Salt et al., 1987; Nin et al., 2008).
Previous studies have shown that Kv7.1 currents were suppressed substantially by increased K+e (Larsen et al., 2011). A plausible mechanism is that K+e stabilizes an inactivated state of the channel; however, the molecular basis remains unknown. Here, we aimed to address the molecular basis of the regulation of Kv7.1 by K+e. We identified a negatively charged amino acid residue, glutamate at position 290 (E290), located in the S5-pore linker of human Kv7.1 (hKv7.1), as a potential regulatory site by K+e. Neutralization of the negatively charged amino acid by cysteine (C) substitution (E290C) significantly decreased the effects of K+e. We further demonstrated that modification of E290C by negatively charged MTS reagents restored the sensitivity of Kv7.1 to K+e, supporting the essential role of the negative charge at this position in sensing K+e. Our findings provide molecular insights into the modulation of Kv7.1 by K+e. Regulation of Kv7.1 by K+e may allow the precise control of the extent of K+ flux across the apical membrane of MCs to regulate the magnitude of the endocochlear potential.
MATERIALS AND METHODS
Isolation of mouse stria vascularis
The study was performed in accordance with the guidelines of the Institutional Animal Care and Use Committee of the University of Nevada, Reno. Postnatal day 12–14 C57BL/6J mice were used for the study. The animals were killed, and the temporal bones were removed and placed in saline solution containing (mM): 90 NaCl, 50 lactobionic acid, 5 KCl, 10 HEPES, 1.2 MgCl2, 1.13 Ca2+ lactobionate, and 5 d-glucose, pH 7.4 with NaOH. The stria vascularis was identified and carefully dissected using fine forceps. The stria vascularis was digested with 1 mg/ml protease type XXIV at 37°C for 10 min. The tissue was transferred to saline solution containing 1 mg/ml bovine serum albumin and 20 µg/ml DNase I for 3 min. The semi-intact epithelia preparation was then washed, placed on a slide, and anchored in the recording chamber, which was mounted on the stage of an inverted microscope (BX50W1; Olympus).
Generation of mutant forms of hKv7.1 channels
WT hKv7.1 clone (GenBank accession no. AF000571) (Sharma et al., 2004) was subcloned into a pIRES2-EGFP plasmid vector (Takara Bio Inc.). Single-point amino acid substitution at the E290 position into K (lysine), A (alanine), C (cysteine), and D (aspartic acid), and single-point amino acid substitution at E284, D286, and E295 positions into C were generated using a QuickChange II mutagenesis kit (Agilent Technologies) and verified by automated sequencing.
Cell culture and transfection of hKv7.1
Chinese hamster ovary (CHO) cell line was used to express the hKv7.1 WT and mutant (MT) channels. CHO cells were maintained in F-12 media with 10% FBS and 1× antibiotic–antimycotic mixture (Invitrogen) at 37°C with 5% CO2. CHO cells were plated onto 12-mm coverslips in F-12 plus 10% FBS without antibiotics and cultured 12–24 h before transfection. Cells were transfected with the hKv7.1-WT or hKv7.1-MT plasmids (200 ng/well) using Lipofectamine 2000 (Invitrogen). For cotransfection of Kv7.1 or E290C with KCNE1, we used a 2:1 quantitative ratio to control the stoichiometry of the assembled channels, as reported recently (Plant et al., 2014). However, the transfection with a 2:1 ratio of DNA of KCNQ1/KCNE1 does not necessarily mean that there will be a 2:1 ratio of KCNQ/KCNE1 subunits in the functional channel.
Whole-cell current recordings in MCs and CHO cells were performed using an Axopatch 200B amplifier, Digidata 1332 digitizer, and pClamp 8 software (Molecular Devices). All the recordings were performed at room temperature. The series resistance was compensated by ∼90% during the recordings. A stock solution of linopirdine, chromanol 293B, and niflumic acid was made in 0.3 M DMSO and stored at −80°C. Current traces were generated using a family of voltage steps from −90 to 60 mV (10-mV increments) for 1.5 or 2.5 s, followed by a step pulse to either −30 or −80 mV from a holding potential of −70 mV. The reversal potential of the K+ current was determined in each experiment using an instantaneous tail current protocol elicited from a holding potential of −70 mV by applying a prepulse of 40 mV for 1.5 s before stepping to a family of test potentials from −110 to −10 mV (10-mV increments) and analyzing the resulting deactivation currents.
For MC recordings, patch electrodes contained (mM): 140 K+-gluconate, 5 KCl, 0.1 CaCl2, 10 HEPES, 5 EGTA, and 5 K2ATP, pH 7.2 with KOH. The external bath solution was constantly perfused (2–3 ml/min) and contained (mM): 90 NaCl, 50 lactobionic acid, 0–150 KCl, 1.13 Ca2+ lactobionate, 1.2 MgCl2, 0.1 niflumic acid, 10 HEPES, and 10 d-glucose, pH 7.4 with NaOH. In some experiments, the K+e ranged from 0 (no K+ added in external solution) to 150 mM. To vary K+ concentrations, Na+ was exchanged 1:1 for K+.
Recordings from CHO cells were performed using patch pipettes with solution consisting of (mM): 145 KCl, 0.1 CaCl2, 2 MgCl2, 1 EGTA, 5 K2ATP, 0.5 GTP, and 10 HEPES, pH 7.4 with KOH. The external bath solution was constantly perfused (2–3 ml/min) and contained (mM): 145 NaCl, 1–50 KCl, 1.8 CaCl2, 0.5 MgCl2, 5 d-glucose, and 10 HEPES, pH 7.4 with NaOH. To vary K+ concentrations, Na+ was exchanged 1:1 for K+.
The stock solution of MTS reagents (Toronto Research Chemicals) was prepared in distilled water (0.3 M) and stored at −80°C. Aliquots of the stock solution were kept at ∼1–2°C on ice, and the working solution containing 1 mM of the MTS reagents was reconstituted immediately before use. The reconstituted solution was discarded if not used within 5 min. Additionally, the thawed stock solution was discarded at the end of the day. DTT stock solution (0.5 M, stored at −20°C) was thawed and diluted to 5 mM in bath solution immediately before use.
Curve fits and data analyses were performed using Origin software (MicroCal, LLC). We used the Goldman–Hodgkin–Katz (GHK) equation to calculate the expected current magnitude, given an internal K+ concentration of 145 mM and varying K+e as outlined (Larsen et al., 2011). The ratio of the currents resulting from the two different K+e can be estimated by the GHK equation as follows:(1)where I1 and I2 represent the K+ currents generated in two different configurations of K+ concentrations (C1, C2) across the cell membrane, KiC1/KoC1 and KiC2/KoC2; Ki and Ko represent K+ concentrations inside and outside, respectively; V is the membrane potential; F is Faraday’s number; R is the gas constant; and T is the absolute temperature.
The steady-state current of WT hKv7.1 at 40 mV in the presence of certain concentrations of K+e was normalized to that of 1 or 4 mM K+e to demonstrate the relationship between the relative magnitude of the hKv7.1 current and K+e concentration ([K+]e). The normalized current, which represents the unblocked fraction (FUB), was fitted to a Langmüir function as follows:(2)where K1/2 is the [K+]e that effectively blocks 50% of the recorded current.
The steady-state activation curves were fitted with a one-state Boltzmann function. Where appropriate, we presented data in the form of mean ± SD. Significant differences between groups were tested using paired/unpaired Student’s t test.
Inhibition of Kv7.1 currents by K+e
As demonstrated previously in expressed hKv7.1 in Xenopus laevis oocytes (Larsen et al., 2011), the application of different concentrations of K+e to hKv7.1 channels expressed in CHO cells yielded K+ current whose magnitude was highly sensitive to K+e in a manner that could not be reconciled with the GHK prediction (Fig. 1, A–C). The blue line in Fig. 1 B represents the fit of hKv7.1 current by a Langmüir function. K1/2 was calculated to be 41 ± 7 mM (n = 7).
To further assess the extent of the apparent K+e-mediated inactivation, we measured the ratio of the amplitude of the steady state to the peak whole-cell K+ current as illustrated in Fig. 1 D. There was an enhanced inactivation by the increased K+e.
Identification of a potential amino acid residue in Kv7.1 for sensing the K+e
A variety of hKv channels have been reported to respond disparately toward elevated K+e. For example, besides the expected current magnitude reduction as predicted by the GHK equation, hKv1.5 and hKv4.3 are insensitive to increasing K+e (Larsen et al., 2011). In contrast, Kv11.1 activity is exquisitely sensitive to changes in K+e (Sanguinetti et al., 1995). The extracellular loops of Kv7.1 consist of several negatively charged amino acid residues. We focused on subtle changes in the sequence of the S5-pore linker among members of the Kv channels. Amino acid sequence alignment of S5-pore linkers of Kv1.5, Kv4.3, and Kv7.1 revealed three negatively charged amino acids at equivalent positions at 284, 286, and 295. Moreover, Kv7.1 is endowed with an additional negatively charged amino acid at position 290 (E290), making the residue unique (Fig. 2 A, middle). The extracellular location of E290 shown in Fig. 2 A (left and right) is based on a structural model of the transmembrane domain of Kv7.1 and the open-state crystal structure of Kv1.2 (Smith et al., 2007).
The negative charge at 290 or equivalent position is only found in Kv7.1, its related member Kv7.3, as well as in Kv10.1 and Kv10.2 (EAG channels) among all the Kv channels. It is not found in Kv1.5 and Kv4.3, which are insensitive to increasing K+e. Therefore, we hypothesize that E290 may serve as a sensor for K+e. Indeed, a point mutation, E290K, in hKv7.1 has been shown to be linked to LQTS in patients (Tester et al., 2005), although information as to whether these patients had hearing loss is unavailable.
To address the molecular basis of K+e-mediated current reduction, we generated the previously reported LQTS mutant (E290K) as well as a point mutation of E290 to a neutral amino acid alanine (E290A). CHO cells transfected with green fluorescence protein only were used as controls (Fig. 2 B). No measurable outward currents were detected in CHO cells transfected with either E290K or E290A (Fig. 2, D–F) compared with WT channel (Fig. 2 C). Our data supported E290K as the pathogenic disease-causing variant in LQTS patients.
We have also used the instantaneous tail current protocol to further assess the reversal potential of WT Kv7.1 current. As shown in Fig. 2 G using 4 mM K+e, the current reversed at −84 mV, close to the calculated K+ equilibrium potential (EK) of −91 mV.
The effect of K+e on E290C current approximated the values predicted by the GHK equation
Mutating E290 to cysteine (C) in hKv7.1 (E290C) gave rise to a functional channel, albeit with a significantly reduced current magnitude compared with the WT current (Fig. 3 A). The steady-state half-activation voltage (V1/2) of the E290C current was shifted by ∼7 mV to the right compared with that of the WT channel (Fig. 3 B). Increased K+e decreased the E290C current (Fig. 3 C, left); however, the ratio of the steady state to the peak currents (Fig. 3 C, right) was significantly increased compared with that of the WT channels, showing a reduced apparent inactivation in the E290C current. Moreover, the effect of K+e on E290C current approximated the values predicted by the GHK equation (Fig. 3 D). The data from the WT channel were replotted in Fig. 3 D for comparison.
The essential role of the negative charge at the 290 position of Kv7.1 in sensing K+e revealed by MTS modifications
We further hypothesize that restoration of the negative charge at E290C may increase the current amplitude and K+e sensitivity. Therefore, we modified the E290C mutant channel using the negatively charged MTS compound, 2-sulfonatoethyl MTS (MTSES). Modification of E290C by MTSES increased the magnitude of the current, as shown in Fig. 4 (A and C). In contrast, the positively charged MTS reagent 2-aminoethyl MTS (MTSEA) had the opposite effects on the current magnitude (Fig. 5, A and C). To confirm the role of the negative charge at position 290, we used another negatively charged 5-sulfonatopentyl MTS (MTSPeS) and positively charged 2-(trimethylammonium) ethyl MTS (MTSET) to modify E290C and observed similar patterns for the current alteration (Fig. 4, B and D, for MTSPeS and Fig. 5, B and D, for MTSET, respectively). None of these MTS reagents had any appreciable effects on the WT channels (Figs. 4 and 5).
We reasoned that if indeed the negatively charged residue at position 290 is responsible for K+e-mediated current reduction with increasing K+e concentrations, modification of E290C by negatively charged MTSES may restore the K+e sensitivity. Fig. 6 A shows the E290C current before (left) and after (middle) MTSES modifications. The right panel further shows the K+e effects (50 mM) on the MTSES-modified E290C. The sensitivity of the MTSES-modified E290C to K+e approximated that of the WT channel, as shown in Fig. 6 B. These findings suggested that the negative charge at the 290 position is essential for channel function and for the sensing of K+e.
Effects of K+e on E284C, D286C, and E295C Kv7.1 mutant channels were similar to the WT channels
To further test the unique role of the negative charge at the 290 position (E290), we constructed three additional point mutations by neutralizing the negative charges of E284, D286, and E295 located in the S5-pore linker of Kv7.1 to cysteine (C). As stated early, these three negatively charged amino acids are not unique to Kv7.1 and are also present in the S5-pore linkers of Kv1.5 and Kv4.3. The effects of K+e on E284C, D286C, and E295C are shown in Fig. 7. As demonstrated in the relationships between the relative current magnitude and the K+e, the experimental data of K+e on the three mutant channels were nearly superimposable on that of the WT channels. They were all at odds with predicted values by the GHK equation (Fig. 7 B).
Modifications of E284C, D286C, and E295C mutant channels by MTS reagents did not alter the current profile
Similar experiments as performed for the WT channel and E290C in Figs. 4 and 5 were performed to test the effects of MTSES and MTSEA modification on E284C, D286C, and E295C mutant channels (Fig. 8). The current density–voltage relationships of the three mutants before and after MTSES or MTSEA are shown in Fig. 8 (C and D, respectively). The outward current density was not significantly altered by MTSES and MTSEA modification for all three mutants.
Effects of K+e on K+ current generated by Kv7.1 and KCNE1
The endogenous Kv7.1 channels in MCs may be assembled by Kv7.1 and KCNE1 (minK) subunits. To further test the mechanism of K+e effects on the assembled Kv7.1 channels, we coexpressed WT hKv7.1/KCNE1 and E290C/KCNE1 in CHO cells to examine the mutational and MTSES modification effects on the channel function, as shown in Fig. 9. Fig. 9 A shows the WT hKv7.1/KCNE1 currents before and after MTSES modification. The bottom panel shows the time course of the current resulting from the MTSES modification followed by the application of DTT at 40 mV. The change in current amplitude of E290C/KCNE1 by MTSES modification is shown in Fig. 9 B. The increase in current amplitude from MTSES modification was reversed by DTT, as shown in the bottom panel. The ratios of the current before and after MTSES modification are shown in Fig. 9 C. We observed a significant increase in current magnitude of the E290C/KCNE1 channel after MTSES modification, similar to the E290C channel expressed alone, as shown in Fig. 4 (A and C). The sensitivity of the channels to K+e was further tested for the WT hKv7.1/KCNE1 and MTSES-modified E290C/KCNE1 channels, as shown in Fig. 9 (D and E). Higher [K+]e results in the reduction of current for the WT hKv7.1/KCNE1 channel reminiscent of the WT hKv7.1 channel. Moreover, MTSES modification of E290C/KCNE1 channel restores the K+e sensitivity comparable to that of WT Kv7.1/KCNE1, as shown in Fig. 9 F.
K+e-induced reduction of the currents in the MCs of the inner ear
Kv7.1 channels have been identified at the apical membrane of MCs and are presumed to be the main channel responsible for the efflux of K+ from MCs into the scala media in the inner ear (Wangemann, 2002; Nin et al., 2008). Indeed, null or missense mutations in Kv7.1 result in profound hearing loss as well as LQTS, as seen in Jervell and Lange-Nielsen syndrome (Jervell and Lange-Nielsen, 1957; Barhanin et al., 1996; Zheng et al., 1999; Johnson et al., 2000; Casimiro et al., 2004).
To this end, we directly tested the effects of K+e on the whole-cell K+ currents recorded from MCs in an intact strip of stria vascularis, as shown in Fig. 10 (A–C). The recorded current using 5.4 mM K+e was found to be sensitive to linopirdine (Fig. 10 E), a known blocker of Kv7 channels (Leão et al., 2009; Anderson et al., 2013). The half-blocking concentration is 4.25 ± 0.06 µM (n = 5). To validate that the major portion of the current was mediated by Kv7.1, we tested the sensitivity of the current to a specific Kv7.1 blocker, chromanol 293B (Fig. 10 F), a known blocker of the Kv7.1 channel (Robbins, 2001; Lerche et al., 2007; Anderson et al., 2013). There was a dose-dependent inhibition of the current by chromanol 293B (Fig. 10 G). Collectively, the data suggest that the majority of the whole-cell K+ currents in MCs are mediated by Kv7.1. More importantly, the Kv7.1-mediated currents from MCs were inhibited by the increased K+e, as shown in Fig. 10 (A–C). Using the GHK equation, we calculated the current magnitude at 40 mV and compared it with the experimental data (Fig. 10 D). The currents from the GHK prediction were at odds with the experimental data, in line with the hetero-expressed hKv7.1 channel shown in Fig. 1 B.
The endolymph of the inner ear is unique compared with other extracellular fluid in its high K+ concentration. This paper provides a molecular account of the handling of K+e by Kv7.1. In the cochlear duct where Kv7.1 channels in MCs are exposed to the endolymph, which consists of ∼150 mM K+, it would be expected that the sensitivity of the channel to K+e would be extremely important in K+ regulation. High K+e-induced current reduction in Kv7.1 has been reported (Larsen et al., 2011), suggesting that the K+e stabilizes an inactivated state in Kv7.1 channels. However, the molecular basis remains unknown.
Functional roles of E290 in the S5-pore linker of Kv7.1 in K+e inhibition of the channel
Previous reports have identified the turret region of the outer mouth of the S5-pore linker as an important regulator of inactivation of the Shaker K+ channel (Schönherr and Heinemann, 1996). Compared with the EAG-related family of K+ channels, e.g., Kv11.1, there are significant differences in the S5-pore linker in that the Kv11.1 has a much longer linker between the outer helix of the pore domain and the pore helix, consisting of ∼40 residues, as opposed to 12–15 residues in other members of the Kv channels (Liu et al., 2002; Torres et al., 2003). We focused on subtle changes in the sequence of the S5-pore linker among members of the Kv channels. We identified a key residue, E290, which is located at the extracellular S5-pore linker of Kv7.1, to be essential for sensing K+e. However, we cannot rule out the possibility that other residues may also be important in sensing K+e.
The outcomes of MTS modifications of E290C provide additional evidence to implicate the negative charge at position 290 as the key regulator in K+e-mediated current reduction. It is possible that the binding and neutralization of the negatively charged residue E290 by K+e produce a conformational change that leads to a slower transition rate from the inactivated state of the channel, as suggested by a previous study demonstrating that K+e stabilizes an inactivated state in Kv7.1 channels (Larsen et al., 2011). Our cysteine-scanning mutagenesis study supports the notion that E290 is accessible from the extracellular side of the channels, thus allowing binding to the K+ ions. Moreover, neutralization of the negative charge by cysteine substitution results in a decrease of the outward current, consistent with increased propensity of the channels in the inactivated state. An alternative possibility for the current reduction by high K+e is the direct binding of K+ ions to the negatively charged residue, leading to the block of the channels. This second possibility remains unlikely based on the results of the previous study (Larsen et al., 2011).
Our findings are in keeping with previous reports, but they are in contrast with reports showing that coexpression of Kv7.1 and KCNE1 in Xenopus oocytes suffices to eliminate K+e-mediated reduction of the IKs (Larsen et al., 2011). One likely possibility for the disparate findings may result from the use of different expression systems. Xenopus oocytes have endogenous KCNE1 and overexpression of the β relative to the α subunit can yield markedly different biophysical outcomes (Seebohm et al., 2001). In our study, the molar ratio for Kv7.1/KCNE1 was calculated based on the recently reported stoichiometry of 2:1 (Plant et al., 2014), reducing potential artifacts emanating from heterologous expression systems.
The role of Kv7.1 channels in the generation of the endocochlear potential
Assuming the resting membrane potential (Vrest) of hair cells of approximately −60 mV, the direct-current potential of ∼140 mV ensues from the endocochlear potential (∼80 mV), which generates a standing current, forming the basis for the mechanoelectrical “battery theory” of cochlear transduction (von Békésy, 1951; Salt et al., 1987; Nin et al., 2008). In contrast, in most cells, the external potential (ground) is 0 mV. Thus, nonselective cationic channels in cells rely on their Vrest as the electromotive force for ion influx. Previous studies have shown that the endocochlear potential is generated by the high K+ throughput across the apical membrane of intermediate cells into an ∼15-nm high impedance space called interstrial space (Salt et al., 1987; Nin et al., 2008). Moreover, the persistent K+ flux across the MCs is not necessarily the generator of the endocochlear potential. The continuous flow of K+ in the cochlear duct is essential for normal cochlear function because it prevents K+ accumulation in the perilymph, curtailing sustained hair cell and neuronal membrane depolarization (Spicer and Schulte, 1998).
Possible functional roles of K+e inhibition of Kv7.1 in MCs of the stria vascularis
Kv7.1 and KCNE1 have been localized in cells of the stria vascularis, and the current mediated by Kv7 channels has been implicated in K+ secretion into the endolymph (Sakagami et al., 1991; Marcus and Shen, 1994; Wangemann et al., 1996; Neyroud et al., 1997; Nicolas et al., 2001). A requirement for Kv7.1 current is further supported by the deafness and vestibular defects found in Kcnq1−/− and Kcne1−/− mice (Vetter et al., 1996; Zheng et al., 1999; Johnson et al., 2000; Casimiro et al., 2004). If Kv7.1 indeed is expressed along the apical membrane of MCs as proposed (Wangemann, 2002), the properties of the channel described in this paper would be expected to have marked physiological ramifications. In prehearing, cochlear duct Kv7.1 in MCs is likely to operate at optimum conditions (Rybak et al., 1992; McGuirt et al., 1995). As endolymph K+ concentration increases during inner ear development, it is predicted that a sizable number of Kv7.1 channels would be confined in the inactivated state, preventing excessive K+ accumulation in the endolymph. As in many biological systems, this negative feedback scenario would be an exquisite strategy to control the flow of K+ and maintain the endocochlear potential of the cochlear duct.
We thank members of our laboratory for comments on the manuscript.
This work was supported by grants from the National Institutes of Health (NIH; DC007592 and DC010386 to E.N. Yamoah), the NIH/National Heart, Lung, and Blood Institute (R01 HL085727 and R01 HL085844 to N. Chiamvimonvat), the VA Merit Review Grant (I01 BX000576 to N. Chiamvimonvat), and the American Heart Association Western States Affiliate Beginning Grant-in-Aid (14BGIA18870087 to X.-D. Zhang).
The authors declare no competing financial interests.
Angus C. Nairn served as editor.
- Abbreviations used in this paper:
- Chinese hamster ovary
- human voltage-gated K+
- external K+
- voltage-gated K+
- long QT syndrome
- marginal cell
- 2-aminoethyl MTS
- 2-sulfonatoethyl MTS
- 2-(trimethylammonium) ethyl MTS
- 5-sulfonatopentyl MTS
- Submitted: 26 August 2014
- Accepted: 27 January 2015
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