January 2016 | Volume 147, No. 1
- Gating the glutamate gate of CLC-2 chloride channel by pore occupancy
Intracellular permeant anions, and not extracellular protons, are the predominant driver of fast gating in the hyperpolarization-activated CLC-2 chloride channel.
- R4496C RyR2 mutation impairs atrial and ventricular contractility
A ryanodine receptor 2 mutation associated with catecholaminergic polymorphic ventricular tachycardia renders cardiomyocytes incapable of mediating a positive inotropic response.
- Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function
¡Vive la différence! In cardiac contraction, the reduction in sarcomere length—rather than length itself—determines contractile force.
Methods and Approaches
- bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles
KvAP conjugated to beads via a C-terminal His-tag seeds formation of a supported bilayer with unidirectional channel orientation for functional studies.