The freezing point depression of freshly excised frozen tissues, pulverized in a hydraulic press or in a mortar, is greater than that of plasma. Even at 0°C. the freezing point depression of such homogenates increases significantly with time. Dilution data indicate that such freezing point data are valid. The presence of intact cells has been shown in smears of tissues pulverized in a mortar, but not in smears of those crushed in a hydraulic press. The osmolarity of various diluent solutions affects the calculated osmotic activity of tissue homogenates presumably because of delayed diffusion between the diluent and cell fluid. With a hypertonic NaCl diluent, spuriously low values of tissue osmotic activity are found from calculations assuming instantaneous mixing between homogenates and diluents. The limitations of data from cryoscopic experiments and from tissue-swelling experiments are discussed in relation to the basic question of whether or not cell fluid is isotonic to extracellular fluid.
Article|
November 20 1956
THE FREEZING POINT DEPRESSION OF MAMMALIAN TISSUES IN RELATION TO THE QUESTION OF OSMOTIC ACTIVITY OF CELL FLUID
William A. Brodsky,
William A. Brodsky
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
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Johannes W. Appelboom,
Johannes W. Appelboom
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
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Warren H. Dennis,
Warren H. Dennis
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
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Warren S. Rehm,
Warren S. Rehm
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
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John F. Miley,
John F. Miley
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
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Israel Diamond
Israel Diamond
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
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William A. Brodsky
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
Johannes W. Appelboom
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
Warren H. Dennis
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
Warren S. Rehm
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
John F. Miley
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
Israel Diamond
From The Departments of Pediatrics and Physiology, University of Louisville School of Medicine, Department of Chemistry Graduate School, University of Louisville, and the Department of Pathology, Children's Hospital, Louisville
Received:
March 16 1956
Online ISSN: 1540-7748
Print ISSN: 0022-1295
Copyright, 1956, by The Rockefeller Institute for Medical Research
1956
J Gen Physiol (1956) 40 (2): 183–199.
Article history
Received:
March 16 1956
Citation
William A. Brodsky, Johannes W. Appelboom, Warren H. Dennis, Warren S. Rehm, John F. Miley, Israel Diamond; THE FREEZING POINT DEPRESSION OF MAMMALIAN TISSUES IN RELATION TO THE QUESTION OF OSMOTIC ACTIVITY OF CELL FLUID . J Gen Physiol 20 November 1956; 40 (2): 183–199. doi: https://doi.org/10.1085/jgp.40.2.183
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