Channel domain | Transition | Forward rate | Reverse rate |

DI L689I | A↔I1 | 1.11E-04 | 4.45E-04 |

I1↔I2 | 3.97E-05 | 2.24E-05 | |

I2↔I3 | 3.33E-04 | 9.48E-01 | |

DII L689I | A↔I1 | 4.91E-05 | 3.56E-04 |

I1↔I2 | 4.79E-05 | 3.03E-05 | |

I2↔I3 | 6.19E-09 | 4.91E-02 | |

DIII L689I | A↔I1 | 2.18E-04 | 4.63E-04 |

I1↔I2 | 3.82E-05 | 2.38E-05 | |

I2↔I3 | 2.97E-04 | 3.55E-03 | |

DIV L689I | A↔I1 | 2.13E-04 | 3.99E-04 |

I1↔I2 | 1.83E-05 | 2.27E-05 | |

I2↔I3 | 4.88E-04 | 9.91E-01 |

The discrete-state Markov models used in Fig. 8 in our companion paper (Silva and Goldstein, 2013) are parameterized for the forward and reverse rate transitions between states A and I

_{1}, states I_{1}and I_{2}, and states I_{2}and I_{3}for the L689I mutation in each of the voltage-sensor domains.