Table 2.

Negative fixed charge density (molarity) of channels

Channel100 mM KCl250 mM KClMean
WT (PA63)70.700.850.78
(PA63E302S)70.600.830.72
(PA63E343S)70.680.800.74
(PA63D276S)70.700.740.72
(PA63D335S)70.580.770.68
(PA63E308S)70.550.720.64
(PA63D315S)70.500.630.57
(PA63F427A)70.380.480.43
  • Negative fixed charge density (molarity) of channels (calculated from Eq. 1) with [KCl]trans = 100 and 250 mM KCl. Each entry is the value of [N] that fits the data in Figs. 2 and 3.