TABLE III

Characteristics of pBoc and Intestinal Ca2+ Oscillations in rde-1(ne219) and rde-1(ne219);kbEx200 Worms

ExperimentBrood sizepBoc periodpBoc CVCa2+ oscillation periodCa2+ oscillation CV
no. eggs/4 ds%s%
rde-1(ne219);143 ± 16 (5)48 ± 2 (6)8 ± 1 (6)NDND
rde-1(ne219);plc-3(RNAi)114 ± 22 (5)55 ± 2 (6)6 ± 1 (6)NDND
rde-1(ne219);kbEx200134 ± 8 (5)47 ± 3 (6)7 ± 1 (6)66 ± 13 (4)9 ± 2 (4)
rde-1(ne219);kbEx200; plc-3(RNAi)122 ± 16 (5)75 ± 3a (10)27 ± 3b (10)52 ± 21 (7)29 ± 3b (7)
rde-1(ne219);kbEx200; egl-8(RNAi)ND50 ± 9 (6)32 ± 2b (6)71 ± 12 (4)33 ± 2b (4)
rde-1(ne219);kbEx200; itr-1(RNAi)NDccNDND
  • a P < 0.01 compared with rde-1(ne219);kbEx200 control worms.

  • b P < 0.001 compared with rde-1(ne219);kbEx200 control worms, unfed rde-1(ne219) worms, or rde-1(ne219) worms fed plc-3 dsRNA.

  • c pBoc was analyzed in six rde-1(ne219);kbEx200; itr-1(RNAi) worms. No pBocs were detected in four of the worms during a 20-min observation period. The remaining two worms had relatively normal pBoc cycles. This variability may reflect the presence of mosaicism (e.g., Yochem and Herman, 2003) and/or an incompletely penetrant RNAi effect.

  • Values are means ± SEM (n = number of worms or intestines). Fluorescence images were acquired at 5-s intervals.