TABLE IV

Effect of PMA and Loss of Diacylglycerol Kinase Activity on egl-8(n488)- or egl-8(RNAi)-induced pBoc Arrhythmia

ExperimentpBoc coefficient of variance (%)
egl-8(n488) exposed to 0.1 μM PMAa 48 ± 8 (6)
egl-8(RNAi) 39 ± 5 (6)
dgk-1(sy428) 12 ± 2 (5)
egl-8(RNAi);dgk-1(sy428) 57 ± 7 (5)
dgk-2(gk124) 7 ± 2 (6)
egl-8(RNAi);dgk-2(gk124) 30 ± 4 (6)
dgk-3(gk110)14 ± 4b (6)
egl-8(RNAi);dgk-3(gk110)29 ± 7 (6)
  • a The frequency of pBoc in egl-8(n488) worms exposed to PMA was severely reduced. pBoc rhythm was therefore quantified over a fixed 20-min observation period. In the six worms examined, two to nine pBocs were detected. For all other worms, pBoc rhythm was quantified over 10 cycles. Values are means ± SEM (n = number of worms).

  • b P < 0.05 compared with N2/glo-1 control animals.