TABLE I

Compositions of Bath Solutions

Ca2+ solutions
NMDG-ClMethanesulfonateCaCl2EDTAEGTAHEDTANTA
mMmMmMmMmMmMmM
Near-zero Ca2+13510
20 nM Ca2+128.53.2410
40 nM Ca2+125.24.910
100 nM Ca2+127.63.710
300 nM Ca2+122.26.410
1 μM Ca2+132.41.310
10 μM Ca2+128.83.110
100 μM Ca2+131.41.825
Low Cl20111.41.825
  • All solutions contained 20 mM HEPES, and pH was adjusted to 7.2 with NMDG. HEDTA, N-hydroxyethylethylenediaminetriacetic acid; NTA, nitrilotriacetic acid.