TABLE III

Affinity Constants for A713X Homomeric Channels

Ki' @ −60 mVKi' (0 mV)δΔG°
μMμMkJ/mol
Amiloride
    A713wt0.76 ± 0.16 (5)2.28 ± 0.600.47 ± 0.05−31.9
    A713G1.89 ± 0.10 (6)a6.50 ± 0.560.54 ± 0.04−29.3
    A713C0.51 ± 0.01 (6)1.86 ± 0.090.55 ± 0.01−32.4
    A713T2.35 ± 0.39 (7)a8.47 ± 0.960.53 ± 0.05−28.6
    A713D2.10 ± 0.15 (8)a6.24 ± 0.360.46 ± 0.01−29.4
    A713V0.95 ± 0.17 (6)2.96 ± 0.410.52 ± 0.01−31.2
    A713C+MTSES0.59 ± 0.07 (6)1.51 ± 0.220.36 ± 0.03−32.9
Benzamil
    A713wt0.15 ± 0.01 (8)0.28 ± 0.030.26 ± 0.06−37.0
    A713T0.83 ± 0.14 (5)a2.62 ± 0.030.56 ± 0.03b−31.5
Benzamidine
    A713wt108 ± 3 (4)576 ± 710.73 ± 0.01−18.3
    A713T196 ± 40 (7)497 ± 870.40 ± 0.05b−18.7
  • Affinity constants, Ki', given as mean ± SEM (n). One-way ANOVA for Ki(−60 mV) (excluding A713C channels treated by MTSES): amiloride, F(5,32) = 12.73, P < 1e-06; benzamil, F(1,11) = 39.03, P < 0.0001; benzamidine, F(1,9) = 3.30, P = 0.103. One way ANOVA for δ: amiloride, F(5,32) = 1.78, P = 0.15; benzamil, F(1,11) = 15.04, P < 0.005; benzamidine, F(1,7) = 19.18, P < 0.005.

  • a P < 0.01; pairwise Dunnet's test with respect to wild type in each dataset.

  • b P < 0.005; pairwise Dunnet's test with respect to wild type in each dataset.