TABLE II

Composition of Extracellular Solutions in mM

nDVF0 CaNominal Ca10 mM Mg10 mM Sr10 mM BaZnNMG1 Ca1 Ca 1 Ba1 Ca 1 Mg
BAPTA02000000000
Divalent00n to 10 Ca010 Sr10 Ba1–10 Znn to 10 Ca1 Ca1 Ca 1 Ba1 Ca
MgCl2000100000001
NaCl1401401401401401401400140140140
NMG-Cl0000000120-140000
HEPES101010101010100101010
  • pH 7.2 (adjusted with NaOH or HCl); osmolarity, 300–310 mOsm (adjusted with sucrose), n = nominal.