TABLE III.

Endogenous channel RIV fingerprint is evident in SLC26A9 + ΔF508CFTR coexpressing HEK 293 cells

ConstructTime
60 s240 s420 s
GFP(± fsk, n.s.)a1.27 ± 0.091.28 ± 0.091.29 ± 0.11
wtCFTR (no fsk)1.27 ± 0.151.36 ± 0.11ntb
wtCFTR(3 min fsk at 240 s)1.30 ± 0.050.98 ± 0.01c0.95 ± 0.01c
SLC26A9 + ΔF508CFTR(3 min fsk at 240 s)1.20 ± 0.051.20 ± 0.071.27 ± 0.08
  • a GFP-alone constructs did not demonstrate significant differences in RIV in the presence or absence of forskolin stimulation, and the data are pooled.

  • b nt, not tested.

  • c P < 0.01 compared to same time point in other constructs.