Table 1. Numbers and densities of channels in native inside-out patches from ramp traces recorded from PE (PA14) and EC (Frag1) giant spheroplasts
Cell typeNo. of MscS per patchNo. of MscL per patchMidpoint ratio pMscS/pMscLMscL midpointPatch radiusPatch areaMscS/µm2MscL/µm2n
mmHgµmµm2
Frag162 ± 6 (1 nS)47 ± 3 (3 nS)0.58 ± 0.02156 ± 41.3 ± 0.111 ± 16 ± 14.4 ± 0.312
PA1413 ± 4 (0.97 nS)78 ± 10 (2.1 nS)0.54 ± 0.02162 ± 131.4 ± 0.213 ± 31.2 ± 0.47 ± 18

A ±15% uncertainty in the absolute values of midpoint tensions for EC and PA MscL may result in an ∼30% error in patch area and a similar error in channel density estimation.