Table 2. Comparison of model outcomes and quantitative summaries of observations
Couplon geometrySimulationsObservations
4 columns × 6 rows2 columns × 6 rowsSurface average, SEMT tubules
Interaction energiesLowHighLowHighLow, less affinityWild typeStac3−/−
Couplon occupancy1.621.811.661.841.411.32,
Couplon occupancy inner feet1.531.84n.a.n.a.n.a.1.71, 0.12n.a.n.a.n.a.
Couplon occupancy border feet1.661.76n.a.n.a.n.a.1.15, 0.08n.a.n.a.n.a.
Full occupancy/feet0.350.400.360.510.310.35, 0.020.300.310.12
Asymmetry2.182.902.212.791.802.45, 0.132.10n.a.n.a.

Couplon occupancy in simulations is the variable co (Eq. 8) averaged over 2 × 107 configurations. In observations it is calculated dividing the number of particles by the total number of tetramers in the couplon. The next two rows in the table limit the calculation to inner or border tetramers. Full occupancy/tetramer in simulations is the variable f(4); in observations, it is the ratio of number of complete tetrads over number of feet or tetramers contained within the couplon borders in the alignment grid. Asymmetry in simulations is the average over the same 2 × 107 configurations of the variable y (Eq. 10). In observations it is the difference between average occupancy of V and C feet (yellow and red squares in Fig. 4 B). Values for T tubules are calculated by our method (left column under WT) or taken from the study by Linsley et al. (2017) of WT (right column under WT) and Stac3−/− zebrafish muscles. n.a., not applicable.