Couplon geometry | Simulations | Observations | |||||||
---|---|---|---|---|---|---|---|---|---|

4 columns × 6 rows | 2 columns × 6 rows | Surface average, SEM | T tubules | ||||||

Interaction energies | Low | High | Low | High | Low, less affinity | Wild type | Stac3−/− | ||

Couplon occupancy | 1.62 | 1.81 | 1.66 | 1.84 | 1.41 | 1.32, 0.06 | 1.24 | 1.70 | 1.26 |

Couplon occupancy inner feet | 1.53 | 1.84 | n.a. | n.a. | n.a. | 1.71, 0.12 | n.a. | n.a. | n.a. |

Couplon occupancy border feet | 1.66 | 1.76 | n.a. | n.a. | n.a. | 1.15, 0.08 | n.a. | n.a. | n.a. |

Full occupancy/feet | 0.35 | 0.40 | 0.36 | 0.51 | 0.31 | 0.35, 0.02 | 0.30 | 0.31 | 0.12 |

Asymmetry | 2.18 | 2.90 | 2.21 | 2.79 | 1.80 | 2.45, 0.13 | 2.10 | n.a. | n.a. |

Couplon occupancy in simulations is the variable *co* (Eq. 8) averaged over 2 × 10^{7} configurations. In observations it is calculated dividing the number of particles by the total number of tetramers in the couplon. The next two rows in the table limit the calculation to inner or border tetramers. Full occupancy/tetramer in simulations is the variable *f*(4); in observations, it is the ratio of number of complete tetrads over number of feet or tetramers contained within the couplon borders in the alignment grid. Asymmetry in simulations is the average over the same 2 × 10^{7} configurations of the variable *y* (Eq. 10). In observations it is the difference between average occupancy of V and C feet (yellow and red squares in Fig. 4 B). Values for T tubules are calculated by our method (left column under WT) or taken from the study by Linsley et al. (2017) of WT (right column under WT) and *Stac3*−/− zebrafish muscles. n.a., not applicable.