- Electrostatic tuning of CFTR’s open states
Gating of the CFTR channel is coupled to ATP hydrolysis such that two open states can be identified under certain conditions. Zhang and Hwang find that pore-lining mutations differentially affect the permeation properties of these open states and suggest that the internal vestibule expands upon ATP hydrolysis.
- Phasic and tonic response mechanisms in RGCs
Visual stimuli of different frequencies are encoded in the retina using transient and sustained responses. Zhao et al. describe the different strategies that are used by four types of retinal ganglion cells to shape photoresponse kinetics.
- Sodium channel block
A number of different drugs block sodium channels, but their mechanism of block is unclear. Tikhonov and Zhorov combine homology modeling with ligand docking and propose a pharmacophore for sodium channel blockers involving cationic and aromatic moieties.
- Substrate-induced trapping of a transporter
A conserved glutamic acid residue is thought to occupy three different conformations in the transport pathway of CLC H+/Cl− exchangers. Vien et al. provide functional evidence that the most central of these three positions is adopted by CLC-ec1 during transport and is stabilized by hydrogen bonds.