- A role for TMEM16B in olfaction
TMEM16B is expressed in olfactory sensory neurons, but previous attempts to establish a physiological role in olfaction have been unsuccessful. Pietra et al. find that genetic ablation of TMEM16B results in defects in the olfactory behavior of mice and the cellular physiology of olfactory sensory neurons.
- EC coupling in single cardiac sarcomeres
Excitation–contraction coupling results in the shortening of many individual sarcomeres along the length of a muscle fiber. Tsukamoto and colleagues develop a technique to quantitatively analyze the dynamics of intracellular calcium transients and length changes at the single sarcomere level.
- pH-sensing sAC controls lysosome pH
Lysosomes are the main degradative compartment in cells and require an acidic luminal environment for correct function. Rahman et al. show that soluble adenylyl cyclase is required for localization of the V-ATPase proton pump to lysosomes and therefore lysosomal acidification and function.
- Purinergic signaling in spermatogonia
Male germ cell development takes place within the testes of mammals, but little is known about its regulation. Fleck et al. record from spermatogonia and Sertoli cells, both in vitro and in situ, and find evidence for P2X4- and P2X7-mediated ATP-gated currents as well as a Ca2+-activated BK conductance.
- Molecular determinants of Si transport
For the aquaporins, selectivity to water is ensured to a large extent by an arginine filter within the pore of the channel. Carpentier et al. find that selectivity to silicon is also controlled by this filter but with the involvement of additional residues that are within or close to the pore.